Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101533 |
| Name | oriT1_pAB228  |
| Organism | Escherichia coli strain DDWI-01 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JAZGLZ010000037 (3435..3857 [+], 423 nt) |
| oriT length | 423 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 423 nt
>oriT1_pAB228
ACTGCGATGCAGGATAGGGCAAACGCCGCAAAATGCTAGCATTTCGCTCGTTTGCCCGATGACCTACAGTCGGTTCTTGTTGGAGAATTTTATGAAATATCAGGTGAAAGAGTTTGTTAATGAAAAATATTCTAAGGCTATTAACATTTTAAAGGATAACCTTAAAGAAAACTATCATATTTTTTATGGAGTAAGATTAAGTGAGATTCTTTTTCCTGCCAGTGAGTATGGCACTGATGCATTCTTTAAAGAGTTTGAATCAATAAATAGTATTACTTTACCATTAATTATTTTTGAAATGAATGAACGTAAGCCTGTAGCTATCATTGGCTTTGAACAAATTGCAGGTGCTGTTTTTGTTGAGCAATTAGACATTAAGGTTTTGGTTATTGACGACCTGTCTGAATTGCTGACTAATGAAAC
ACTGCGATGCAGGATAGGGCAAACGCCGCAAAATGCTAGCATTTCGCTCGTTTGCCCGATGACCTACAGTCGGTTCTTGTTGGAGAATTTTATGAAATATCAGGTGAAAGAGTTTGTTAATGAAAAATATTCTAAGGCTATTAACATTTTAAAGGATAACCTTAAAGAAAACTATCATATTTTTTATGGAGTAAGATTAAGTGAGATTCTTTTTCCTGCCAGTGAGTATGGCACTGATGCATTCTTTAAAGAGTTTGAATCAATAAATAGTATTACTTTACCATTAATTATTTTTGAAATGAATGAACGTAAGCCTGTAGCTATCATTGGCTTTGAACAAATTGCAGGTGCTGTTTTTGTTGAGCAATTAGACATTAAGGTTTTGGTTATTGACGACCTGTCTGAATTGCTGACTAATGAAAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1361 | GenBank | WP_000539535 |
| Name | mobP1_V2H89_RS20530_pAB228 |
UniProt ID | _ |
| Length | 388 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 388 a.a. Molecular weight: 44455.74 Da Isoelectric Point: 10.4644
>WP_000539535.1 MULTISPECIES: MobP1 family relaxase [Enterobacterales]
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAITRQGVRNSI
DYMSRESELPVMSESGQVWTGDEIQEAKEHMIDRANDPQHVINDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLRGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDVGYDHYQNDKTKSKQYFIKLKTLNKGVEKTYWGADF
GDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGIDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFLGL
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAITRQGVRNSI
DYMSRESELPVMSESGQVWTGDEIQEAKEHMIDRANDPQHVINDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLRGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDVGYDHYQNDKTKSKQYFIKLKTLNKGVEKTYWGADF
GDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGIDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFLGL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 989 | GenBank | WP_000053829 |
| Name | t4cp2_V2H89_RS20470_pAB228 |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 69290.20 Da Isoelectric Point: 6.9121
>WP_000053829.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacterales]
MSLKLPDKGQWVFIGLVMCLVTYYAGSVAVYFLNGKTPLYIWKNFDSMLLWKIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFVIWQLNKMDVALYGDAKFASDNDLKKSKLLKWETENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSASQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
MSLKLPDKGQWVFIGLVMCLVTYYAGSVAVYFLNGKTPLYIWKNFDSMLLWKIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFVIWQLNKMDVALYGDAKFASDNDLKKSKLLKWETENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSASQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 17123..27083
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| V2H89_RS20440 (V2H89_20440) | 12915..13256 | - | 342 | WP_001547188 | hypothetical protein | - |
| V2H89_RS20445 (V2H89_20445) | 13267..13800 | - | 534 | WP_330990726 | thermonuclease family protein | - |
| V2H89_RS20450 (V2H89_20450) | 13797..14231 | - | 435 | WP_000981209 | membrane protein | - |
| V2H89_RS20455 (V2H89_20455) | 14313..14570 | - | 258 | WP_000726017 | hypothetical protein | - |
| V2H89_RS20460 (V2H89_20460) | 14576..14878 | - | 303 | WP_000820911 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| V2H89_RS20465 (V2H89_20465) | 14875..15288 | - | 414 | WP_236918563 | cag pathogenicity island Cag12 family protein | - |
| V2H89_RS20470 (V2H89_20470) | 15285..17120 | - | 1836 | WP_000053829 | type IV secretory system conjugative DNA transfer family protein | - |
| V2H89_RS20475 (V2H89_20475) | 17123..18154 | - | 1032 | WP_001058476 | P-type DNA transfer ATPase VirB11 | virB11 |
| V2H89_RS20480 (V2H89_20480) | 18156..19361 | - | 1206 | WP_089602783 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| V2H89_RS20485 (V2H89_20485) | 19358..20287 | - | 930 | WP_000783381 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| V2H89_RS20490 (V2H89_20490) | 20292..21005 | - | 714 | WP_000394613 | type IV secretion system protein | virB8 |
| V2H89_RS20495 (V2H89_20495) | 21250..22383 | - | 1134 | WP_001547182 | type IV secretion system protein | virB6 |
| V2H89_RS20500 (V2H89_20500) | 22393..22620 | - | 228 | WP_000835348 | EexN family lipoprotein | - |
| V2H89_RS20505 (V2H89_20505) | 22621..23379 | - | 759 | WP_000744201 | type IV secretion system protein | - |
| V2H89_RS20510 (V2H89_20510) | 23390..26143 | - | 2754 | WP_001363160 | VirB3 family type IV secretion system protein | virb4 |
| V2H89_RS20515 (V2H89_20515) | 26162..26458 | - | 297 | WP_000040716 | TrbC/VirB2 family protein | virB2 |
| V2H89_RS20520 (V2H89_20520) | 26436..27083 | - | 648 | WP_000953535 | lytic transglycosylase domain-containing protein | virB1 |
| V2H89_RS20525 (V2H89_20525) | 27307..27813 | - | 507 | WP_000872474 | transcription termination/antitermination NusG family protein | - |
| V2H89_RS20530 (V2H89_20530) | 28146..29312 | - | 1167 | WP_000539535 | MobP1 family relaxase | - |
| V2H89_RS20535 (V2H89_20535) | 29315..29860 | - | 546 | WP_000815825 | DNA distortion polypeptide 1 | - |
| V2H89_RS20540 (V2H89_20540) | 30176..30448 | - | 273 | WP_001547192 | hypothetical protein | - |
| V2H89_RS20545 (V2H89_20545) | 30461..30610 | - | 150 | WP_000003876 | hypothetical protein | - |
| V2H89_RS20550 (V2H89_20550) | 30679..30972 | - | 294 | WP_021568250 | hypothetical protein | - |
| V2H89_RS20555 (V2H89_20555) | 30987..31403 | - | 417 | WP_012421208 | tail-associated lysozyme | - |
Host bacterium
| ID | 1977 | GenBank | NZ_JAZGLZ010000037 |
| Plasmid name | pAB228 | Incompatibility group | IncX1 |
| Plasmid size | 31716 bp | Coordinate of oriT [Strand] | 3435..3857 [+]; 29640..30236 [+] |
| Host baterium | Escherichia coli strain DDWI-01 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |