Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101499 |
| Name | oriT_p2165-4  |
| Organism | Escherichia coli strain GN02165 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JALJQH010000002 (3766..4187 [-], 422 nt) |
| oriT length | 422 nt |
| IRs (inverted repeats) | 125..131, 143..149 (AAATATT..AATATTT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 422 nt
>oriT_p2165-4
ACTGCGATGCAGGATAGGGCAAACGCCGCAAAATGCTAGCATTTCGCTTGTTTGCCCGGTGACCTACAGTCGGTTCTTGTTGGAGTGATTTATGAAATATCAGGTTAAAGAGTTTATTAACGATAAATATTCTAAAGCTGTTAATATTTTAAAGGATAACCTTAAAGAACACTACCATGTTTTTATGGTTTGAGATTGAGTGAGATTCTTTTCCCTGCCAGTAAATACGGCTCTGATGCTTTCTTTAATGAGTTCGAAGCAATAAACAGCATTACATTGCCATTAATTATTTTTGAAATGAATGAACGTAAGCCTGTAGCTATCATTGGCTTTGAACAAATTGCAGGTGCTGTTTTTGTTGAACAATTAGACATTAAGGTTTTGGTTATTGATGACTTGTCTGAATTGCTGACCAATGAAAC
ACTGCGATGCAGGATAGGGCAAACGCCGCAAAATGCTAGCATTTCGCTTGTTTGCCCGGTGACCTACAGTCGGTTCTTGTTGGAGTGATTTATGAAATATCAGGTTAAAGAGTTTATTAACGATAAATATTCTAAAGCTGTTAATATTTTAAAGGATAACCTTAAAGAACACTACCATGTTTTTATGGTTTGAGATTGAGTGAGATTCTTTTCCCTGCCAGTAAATACGGCTCTGATGCTTTCTTTAATGAGTTCGAAGCAATAAACAGCATTACATTGCCATTAATTATTTTTGAAATGAATGAACGTAAGCCTGTAGCTATCATTGGCTTTGAACAAATTGCAGGTGCTGTTTTTGTTGAACAATTAGACATTAAGGTTTTGGTTATTGATGACTTGTCTGAATTGCTGACCAATGAAAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1338 | GenBank | WP_032180641 |
| Name | mobP1_MWG53_RS00245_p2165-4 |
UniProt ID | _ |
| Length | 388 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 388 a.a. Molecular weight: 44429.70 Da Isoelectric Point: 10.4862
>WP_032180641.1 MULTISPECIES: MobP1 family relaxase [Enterobacteriaceae]
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAITRQGVRNSI
DYMSRESELPVMSESGQVWTGDEIQEAKEHMIDRANDPQHVINDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLRGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDVGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGIDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFLGL
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAITRQGVRNSI
DYMSRESELPVMSESGQVWTGDEIQEAKEHMIDRANDPQHVINDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLRGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDVGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGIDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFLGL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 962 | GenBank | WP_033550413 |
| Name | t4cp2_MWG53_RS00305_p2165-4 |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 69262.14 Da Isoelectric Point: 6.9121
>WP_033550413.1 type IV secretory system conjugative DNA transfer family protein [Escherichia coli]
MSLKLPDKGQWAFIGLVMCLVTYYAGSVAVYFLNGKTPLYIWKNFDSMLLWKIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFVIWQLNKMDVALYGDAKFASDNDLKKSKLLKWETENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSASQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
MSLKLPDKGQWAFIGLVMCLVTYYAGSVAVYFLNGKTPLYIWKNFDSMLLWKIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFVIWQLNKMDVALYGDAKFASDNDLKKSKLLKWETENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSASQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 14740..24756
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MWG53_RS00215 (MWG53_00215) | 10111..10410 | + | 300 | WP_000650311 | hypothetical protein | - |
| MWG53_RS00220 (MWG53_00220) | 10420..10836 | + | 417 | WP_033550408 | tail-associated lysozyme | - |
| MWG53_RS00225 (MWG53_00225) | 10851..11144 | + | 294 | WP_032180650 | hypothetical protein | - |
| MWG53_RS00230 (MWG53_00230) | 11213..11362 | + | 150 | WP_000003876 | hypothetical protein | - |
| MWG53_RS00235 (MWG53_00235) | 11375..11647 | + | 273 | WP_001363158 | hypothetical protein | - |
| MWG53_RS00240 (MWG53_00240) | 11963..12508 | + | 546 | WP_000815825 | DNA distortion polypeptide 1 | - |
| MWG53_RS00245 (MWG53_00245) | 12511..13677 | + | 1167 | WP_032180641 | MobP1 family relaxase | - |
| MWG53_RS00250 (MWG53_00250) | 14010..14516 | + | 507 | WP_000872474 | transcription termination/antitermination NusG family protein | - |
| MWG53_RS00255 (MWG53_00255) | 14740..15387 | + | 648 | WP_032180638 | lytic transglycosylase domain-containing protein | virB1 |
| MWG53_RS00260 (MWG53_00260) | 15365..15661 | + | 297 | WP_032180636 | TrbC/VirB2 family protein | virB2 |
| MWG53_RS00265 (MWG53_00265) | 15680..18433 | + | 2754 | WP_032180634 | VirB3 family type IV secretion system protein | virb4 |
| MWG53_RS00270 (MWG53_00270) | 18444..19202 | + | 759 | WP_033550410 | type IV secretion system protein | - |
| MWG53_RS00275 (MWG53_00275) | 19203..19490 | + | 288 | WP_000835347 | EexN family lipoprotein | - |
| MWG53_RS00280 (MWG53_00280) | 19499..20629 | + | 1131 | WP_032180631 | type IV secretion system protein | virB6 |
| MWG53_RS00285 (MWG53_00285) | 20874..21587 | + | 714 | WP_059337614 | type IV secretion system protein | virB8 |
| MWG53_RS00290 (MWG53_00290) | 21592..22521 | + | 930 | WP_032180630 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| MWG53_RS00295 (MWG53_00295) | 22518..23723 | + | 1206 | WP_025693452 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| MWG53_RS00300 (MWG53_00300) | 23725..24756 | + | 1032 | WP_033550412 | P-type DNA transfer ATPase VirB11 | virB11 |
| MWG53_RS00305 (MWG53_00305) | 24759..26594 | + | 1836 | WP_033550413 | type IV secretory system conjugative DNA transfer family protein | - |
| MWG53_RS00310 (MWG53_00310) | 26591..27004 | + | 414 | WP_032180624 | cag pathogenicity island Cag12 family protein | - |
| MWG53_RS00315 (MWG53_00315) | 27001..27303 | + | 303 | WP_032180621 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| MWG53_RS00320 (MWG53_00320) | 27358..27606 | + | 249 | WP_032180619 | hypothetical protein | - |
| MWG53_RS00325 (MWG53_00325) | 27710..28027 | + | 318 | WP_033550414 | hypothetical protein | - |
| MWG53_RS00330 (MWG53_00330) | 28024..28515 | + | 492 | WP_033550415 | thermonuclease family protein | - |
Host bacterium
| ID | 1943 | GenBank | NZ_JALJQH010000002 |
| Plasmid name | p2165-4 | Incompatibility group | - |
| Plasmid size | 35387 bp | Coordinate of oriT [Strand] | 3766..4187 [-] |
| Host baterium | Escherichia coli strain GN02165 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |