Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101496
Name   oriT_p5243-2 in_silico
Organism   Escherichia coli strain GN05243
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JALJQN010000004 (57856..57928 [+], 73 nt)
oriT length   73 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 73 nt

>oriT_p5243-2
GTCGGGGCAAAGCCCTGACCAGGTGGGGAATGTCTGAGTGCGCGTGCGCGGTCCGACATTCCCACATCCTGTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1335 GenBank   WP_000991387
Name   Relaxase_MWG52_RS26900_p5243-2 insolico UniProt ID   A0A7I0L2J7
Length   903 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 903 a.a.        Molecular weight: 104567.95 Da        Isoelectric Point: 7.5259

>WP_000991387.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDVPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCNSLETAAEEMQYKADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRYFAERLPEMARNGELESCLDVHRTLAKAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGRNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
TTVQCESLIEDMIAGGNFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEL
QAGPFEIAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNADRCVILCEPGGTPLYEDTGVLEARLQKDGS
VRFRDRRNGELVCVDYGDRVVFYHHQDRNELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAW
HNAAGITGNGHFVISRPDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-315)


  Protein structure


Source ID Structure
AlphaFold DB A0A7I0L2J7


Auxiliary protein

ID   472 GenBank   WP_001291058
Name   WP_001291058_p5243-2 insolico UniProt ID   A0A7I0L241
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12730.52 Da        Isoelectric Point: 9.9656

>WP_001291058.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVSTFIRSSSLDKVVNCRTDDRMIDEIMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITEYVNALRRDLMGR

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A7I0L241


T4CP

ID   958 GenBank   WP_000077664
Name   trbC_MWG52_RS26885_p5243-2 insolico UniProt ID   _
Length   767 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 767 a.a.        Molecular weight: 87040.19 Da        Isoelectric Point: 7.4857

>WP_000077664.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIWPLALPVCLLFTLVTSVIFMLQRWRCP
LRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQYEADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVM
FFATTGGGKTETTFAWLLNPLCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEI
IQSGEKSRPQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSE
TFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFVTQQSMLLARDLGYRLE
GTDAQTLEVKKYKGSFPYICILDEVGAYYTDRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLM
QNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLN
AGEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPEHVSSII
GVLTAKPSRKRRKNRTEPYRIIDTFQRRLATAQTSLDLLPQYDTDIESRANELWKKAVHTINNTTREERR
VCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPDLTASPPHKKNVAQTPSGGKQESRKKRF

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 5847..51202

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWG52_RS26625 (MWG52_26635) 1891..2328 + 438 WP_000539952 type IV pilus biogenesis protein PilM -
MWG52_RS26630 (MWG52_26640) 2360..3979 + 1620 WP_001035562 PilN family type IVB pilus formation outer membrane protein -
MWG52_RS26635 (MWG52_26645) 4000..5295 + 1296 WP_000129883 type 4b pilus protein PilO2 -
MWG52_RS26640 (MWG52_26650) 5285..5743 + 459 WP_000095886 type IV pilus biogenesis protein PilP -
MWG52_RS26645 (MWG52_26655) 5847..7355 + 1509 WP_000880806 ATPase, T2SS/T4P/T4SS family virB11
MWG52_RS26650 (MWG52_26660) 7357..8451 + 1095 WP_032253914 type II secretion system F family protein -
MWG52_RS26655 (MWG52_26665) 8513..9049 + 537 WP_000111521 type 4 pilus major pilin -
MWG52_RS26660 (MWG52_26670) 9094..9579 + 486 WP_001080767 lytic transglycosylase domain-containing protein virB1
MWG52_RS26665 (MWG52_26675) 9595..10221 + 627 WP_013307883 prepilin peptidase -
MWG52_RS26670 (MWG52_26680) 10239..11561 + 1323 WP_052993642 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MWG52_RS26675 (MWG52_26685) 11566..11955 - 390 Protein_13 phage tail protein -
MWG52_RS26680 (MWG52_26690) 12041..12196 + 156 WP_001358489 hypothetical protein -
MWG52_RS26685 (MWG52_26695) 12739..13047 - 309 WP_102166912 pilus assembly protein -
MWG52_RS26690 (MWG52_26700) 13097..14221 + 1125 WP_013307880 site-specific integrase -
MWG52_RS26695 (MWG52_26705) 14434..15255 + 822 WP_001681675 hypothetical protein traE
MWG52_RS26700 (MWG52_26710) 15357..16559 + 1203 WP_013307879 conjugal transfer protein TraF -
MWG52_RS26705 (MWG52_26715) 16663..17121 + 459 WP_025750398 DotD/TraH family lipoprotein -
MWG52_RS26710 (MWG52_26720) 17118..17954 + 837 WP_000745049 type IV secretory system conjugative DNA transfer family protein traI
MWG52_RS26715 (MWG52_26725) 17938..19086 + 1149 WP_013307876 plasmid transfer ATPase TraJ virB11
MWG52_RS26720 (MWG52_26730) 19083..19373 + 291 WP_000817801 IcmT/TraK family protein traK
MWG52_RS26725 (MWG52_26735) 19785..21047 + 1263 WP_000608644 IS1380-like element ISEcp1 family transposase -
MWG52_RS26730 (MWG52_26740) 21297..22172 + 876 WP_001617865 extended-spectrum class A beta-lactamase CTX-M-14 -
MWG52_RS26735 (MWG52_26745) 22207..22662 + 456 Protein_25 IS5 family transposase -
MWG52_RS26740 (MWG52_26750) 22670..23251 + 582 WP_025750394 hypothetical protein -
MWG52_RS26745 (MWG52_26755) 23605..27666 + 4062 WP_013307874 LPD7 domain-containing protein -
MWG52_RS26750 (MWG52_26760) 27683..28033 + 351 WP_001056367 hypothetical protein traL
MWG52_RS26755 (MWG52_26765) 28045..28740 + 696 WP_001681668 DotI/IcmL family type IV secretion protein traM
MWG52_RS26760 (MWG52_26770) 28751..29725 + 975 WP_000547383 DotH/IcmK family type IV secretion protein traN
MWG52_RS26765 (MWG52_26775) 29729..31066 + 1338 WP_013307873 conjugal transfer protein TraO traO
MWG52_RS26770 (MWG52_26780) 31063..31776 + 714 WP_000787003 conjugal transfer protein TraP traP
MWG52_RS26775 (MWG52_26785) 31773..32303 + 531 WP_000987023 conjugal transfer protein TraQ traQ
MWG52_RS26780 (MWG52_26790) 32350..32748 + 399 WP_000088813 DUF6750 family protein traR
MWG52_RS28085 32805..33056 + 252 WP_001277573 hypothetical protein -
MWG52_RS26790 (MWG52_26800) 32971..33789 + 819 WP_000753550 hypothetical protein traT
MWG52_RS26795 (MWG52_26805) 34084..37128 + 3045 WP_001024766 conjugal transfer protein traU
MWG52_RS26800 (MWG52_26810) 37128..37748 + 621 WP_000286854 hypothetical protein traV
MWG52_RS26805 (MWG52_26815) 37706..38911 + 1206 WP_001189548 conjugal transfer protein TraW traW
MWG52_RS26810 (MWG52_26820) 38908..39477 + 570 WP_000650752 conjugal transfer protein TraX -
MWG52_RS26815 (MWG52_26825) 39552..41711 + 2160 WP_000691811 DotA/TraY family protein traY
MWG52_RS26820 (MWG52_26830) 41799..42446 + 648 WP_015056434 plasmid IncI1-type surface exclusion protein ExcA -
MWG52_RS26825 (MWG52_26835) 42720..44306 + 1587 WP_000004564 TnsA endonuclease N-terminal domain-containing protein -
MWG52_RS26830 (MWG52_26840) 44444..44596 - 153 WP_001335079 Hok/Gef family protein -
MWG52_RS26835 (MWG52_26845) 44926..45168 + 243 WP_000650923 hypothetical protein -
MWG52_RS26840 (MWG52_26850) 45256..45447 + 192 WP_000751583 hypothetical protein -
MWG52_RS26845 (MWG52_26855) 45459..45827 + 369 WP_001345852 hypothetical protein -
MWG52_RS26850 (MWG52_26860) 45831..46046 + 216 WP_000266543 hypothetical protein -
MWG52_RS26855 (MWG52_26865) 46244..46867 - 624 WP_001155247 helix-turn-helix domain-containing protein -
MWG52_RS26860 (MWG52_26870) 47026..47493 + 468 WP_000598962 thermonuclease family protein -
MWG52_RS26865 (MWG52_26875) 47643..47819 + 177 WP_001054907 hypothetical protein -
MWG52_RS28090 47882..47977 - 96 WP_001363141 DinQ-like type I toxin DqlB -
MWG52_RS26875 (MWG52_26885) 48774..50081 + 1308 WP_021577249 IncI1-type conjugal transfer protein TrbA trbA
MWG52_RS26880 (MWG52_26890) 50078..51202 + 1125 WP_000152387 DsbC family protein trbB
MWG52_RS26885 (MWG52_26895) 51183..53486 + 2304 WP_000077664 F-type conjugative transfer protein TrbC -
MWG52_RS26890 (MWG52_26900) 53953..54183 + 231 WP_071591569 hypothetical protein -
MWG52_RS26895 (MWG52_26905) 54281..54712 - 432 WP_001335086 cell envelope integrity TolA C-terminal domain-containing protein -

Host bacterium

ID   1940 GenBank   NZ_JALJQN010000004
Plasmid name   p5243-2 Incompatibility group   IncB/O/K/Z
Plasmid size   93942 bp Coordinate of oriT [Strand]   57856..57928 [+]
Host baterium   Escherichia coli strain GN05243

Cargo genes

Drug resistance gene   blaCTX-M-14
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -