Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101489 |
| Name | oriT_MRSN 12115|unnamed  |
| Organism | Citrobacter freundii strain MRSN 12115 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JYFZ02000018 (353..410 [-], 58 nt) |
| oriT length | 58 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 58 nt
>oriT_MRSN 12115|unnamed
GGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCT
GGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1330 | GenBank | WP_046810950 |
| Name | Relaxase_TO64_RS28670_MRSN 12115|unnamed |
UniProt ID | _ |
| Length | 211 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 211 a.a. Molecular weight: 23336.44 Da Isoelectric Point: 8.3767
>WP_046810950.1 relaxase/mobilization nuclease domain-containing protein, partial [Citrobacter freundii]
MIVKFHPRGRGGGGGPVDYLLGKDRQRDGASVLQGKPDEVRELIDASPYAKKYTSGVLSFAEQDLPPGQR
EKLMASFERVLMPGLDKDQYSVLWVEHRDKGRLELNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTIVN
GRLGLHDPNAPENRRVLVSPSALPEAKQEAAQAITSGLLALASSGELKTRQDVTEALESAGFEVVRTTKS
S
MIVKFHPRGRGGGGGPVDYLLGKDRQRDGASVLQGKPDEVRELIDASPYAKKYTSGVLSFAEQDLPPGQR
EKLMASFERVLMPGLDKDQYSVLWVEHRDKGRLELNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTIVN
GRLGLHDPNAPENRRVLVSPSALPEAKQEAAQAITSGLLALASSGELKTRQDVTEALESAGFEVVRTTKS
S
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 465 | GenBank | WP_004193914 |
| Name | WP_004193914_MRSN 12115|unnamed |
UniProt ID | A0A8T3UXZ2 |
| Length | 107 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 107 a.a. Molecular weight: 11783.55 Da Isoelectric Point: 7.8963
>WP_004193914.1 MULTISPECIES: MobC family plasmid mobilization relaxosome protein [Bacteria]
MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSISPALLRQLAGMGNNLNQIARQVNAG
GGSGHDRVQIVAALMAIDAGLERLRHAVLEKGADDDR
MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSISPALLRQLAGMGNNLNQIARQVNAG
GGSGHDRVQIVAALMAIDAGLERLRHAVLEKGADDDR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 466 | GenBank | WP_046810949 |
| Name | WP_046810949_MRSN 12115|unnamed |
UniProt ID | _ |
| Length | 148 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 148 a.a. Molecular weight: 16544.88 Da Isoelectric Point: 10.1030
>WP_046810949.1 MbeB family mobilization protein, partial [Citrobacter freundii]
KSKAQQQTTGEMLKAAFSEHEKSVRAELSESEKRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVL
VSALLIASSAGILWWQGQQILENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDS
SWMILAGK
KSKAQQQTTGEMLKAAFSEHEKSVRAELSESEKRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVL
VSALLIASSAGILWWQGQQILENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDS
SWMILAGK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 1933 | GenBank | NZ_JYFZ02000018 |
| Plasmid name | MRSN 12115|unnamed | Incompatibility group | Col440II |
| Plasmid size | 5018 bp | Coordinate of oriT [Strand] | 353..410 [-] |
| Host baterium | Citrobacter freundii strain MRSN 12115 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |