Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101482
Name   oriT_B7S79|unnamed7 in_silico
Organism   Escherichia coli strain B7S79
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_SJSV01000024 (8158..8244 [-], 87 nt)
oriT length   87 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 87 nt

>oriT_B7S79|unnamed7
GGGGTGTCGGGGCGAAGCCCTGACCAGGAGGCAATTGTATAATCGCGCGTGCGCGGTTATACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1325 GenBank   WP_001354015
Name   nikB_E0E06_RS18765_B7S79|unnamed7 insolico UniProt ID   E8XLT1
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104018.47 Da        Isoelectric Point: 7.3526

>WP_001354015.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCLAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPENRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB E8XLT1


Auxiliary protein

ID   464 GenBank   WP_001281051
Name   WP_001281051_B7S79|unnamed7 insolico UniProt ID   A0A3U8I0C6
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12646.44 Da        Isoelectric Point: 10.4030

>WP_001281051.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSDKRQRSGSERRQKTILRAVRFSPDEDETICKNAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A3U8I0C6


T4CP

ID   943 GenBank   WP_001289276
Name   trbC_E0E06_RS18770_B7S79|unnamed7 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 13623..51922

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E0E06_RS18770 (E0E06_18775) 11339..13630 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
E0E06_RS18775 (E0E06_18780) 13623..14693 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
E0E06_RS18780 (E0E06_18785) 14712..15920 - 1209 WP_000121274 IncI1-type conjugal transfer protein TrbA trbA
E0E06_RS18785 (E0E06_18790) 16212..16364 + 153 WP_001331364 Hok/Gef family protein -
E0E06_RS27040 16436..16627 - 192 WP_174715448 hypothetical protein -
E0E06_RS28015 17188..17283 + 96 WP_000609148 DinQ-like type I toxin DqlB -
E0E06_RS27045 17348..17524 - 177 WP_001054904 hypothetical protein -
E0E06_RS18795 (E0E06_18800) 17916..18125 + 210 WP_000062603 HEAT repeat domain-containing protein -
E0E06_RS18800 (E0E06_18805) 18197..18859 - 663 WP_000653334 plasmid IncI1-type surface exclusion protein ExcA -
E0E06_RS18805 (E0E06_18810) 18924..21086 - 2163 WP_000698351 DotA/TraY family protein traY
E0E06_RS18810 (E0E06_18815) 21183..21767 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
E0E06_RS18815 (E0E06_18820) 21796..22998 - 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
E0E06_RS18820 (E0E06_18825) 22965..23579 - 615 WP_000337398 IncI1-type conjugal transfer protein TraV traV
E0E06_RS18825 (E0E06_18830) 23579..26623 - 3045 WP_001024780 IncI1-type conjugal transfer protein TraU traU
E0E06_RS18830 (E0E06_18835) 26713..27513 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
E0E06_RS18835 (E0E06_18840) 27497..27685 - 189 WP_001277255 putative conjugal transfer protein TraS -
E0E06_RS18840 (E0E06_18845) 27749..28153 - 405 WP_000086957 IncI1-type conjugal transfer protein TraR traR
E0E06_RS18845 (E0E06_18850) 28204..28731 - 528 WP_001055569 conjugal transfer protein TraQ traQ
E0E06_RS18850 (E0E06_18855) 28731..29435 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
E0E06_RS18855 (E0E06_18860) 29435..30724 - 1290 WP_001272005 conjugal transfer protein TraO traO
E0E06_RS18860 (E0E06_18865) 30727..31710 - 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
E0E06_RS18865 (E0E06_18870) 31721..32413 - 693 WP_000138548 DotI/IcmL family type IV secretion protein traM
E0E06_RS18870 (E0E06_18875) 32410..32757 - 348 WP_001055900 conjugal transfer protein traL
E0E06_RS18875 (E0E06_18880) 32775..36542 - 3768 WP_001141529 LPD7 domain-containing protein -
E0E06_RS18880 (E0E06_18885) 36632..37183 - 552 WP_000014583 phospholipase D family protein -
E0E06_RS18885 (E0E06_18890) 37198..37488 - 291 WP_001299214 hypothetical protein traK
E0E06_RS18890 (E0E06_18895) 37485..38633 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
E0E06_RS18895 (E0E06_18900) 38630..39448 - 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
E0E06_RS18900 (E0E06_18905) 39445..39903 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
E0E06_RS18905 (E0E06_18910) 40298..40882 - 585 WP_000977522 histidine phosphatase family protein -
E0E06_RS18910 (E0E06_18915) 40942..42144 - 1203 WP_000976351 conjugal transfer protein TraF -
E0E06_RS18915 (E0E06_18920) 42229..43053 - 825 WP_001238939 conjugal transfer protein TraE traE
E0E06_RS18920 (E0E06_18925) 43204..44358 - 1155 WP_001139957 site-specific integrase -
E0E06_RS27670 44357..44710 + 354 WP_001393368 hypothetical protein -
E0E06_RS18955 (E0E06_18960) 45879..46127 + 249 WP_001349157 hypothetical protein -
E0E06_RS18960 (E0E06_18965) 46124..47416 - 1293 WP_001417545 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
E0E06_RS18965 (E0E06_18970) 47416..48072 - 657 WP_001193553 prepilin peptidase -
E0E06_RS18970 (E0E06_18975) 48057..48617 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
E0E06_RS18975 (E0E06_18980) 48627..49241 - 615 WP_000959785 type 4 pilus major pilin -
E0E06_RS18980 (E0E06_18985) 49259..50356 - 1098 WP_001208805 type II secretion system F family protein -
E0E06_RS18985 (E0E06_18990) 50369..51922 - 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
E0E06_RS18990 (E0E06_18995) 51933..52385 - 453 WP_001247336 type IV pilus biogenesis protein PilP -
E0E06_RS18995 (E0E06_19000) 52372..53667 - 1296 WP_000752774 type 4b pilus protein PilO2 -
E0E06_RS19000 (E0E06_19005) 53660..55342 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
E0E06_RS19005 (E0E06_19010) 55356..55793 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
E0E06_RS19010 (E0E06_19015) 55793..56860 - 1068 WP_000742600 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   1926 GenBank   NZ_SJSV01000024
Plasmid name   B7S79|unnamed7 Incompatibility group   IncI1
Plasmid size   66695 bp Coordinate of oriT [Strand]   8158..8244 [-]
Host baterium   Escherichia coli strain B7S79

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -