Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101456
Name   oriT_pCRE17_5 in_silico
Organism   Klebsiella michiganensis strain S17_CRE17
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAHBNO010000007 (20378..22081 [-], 1704 nt)
oriT length   1704 nt
IRs (inverted repeats)      655..664, 672..681  (TTTTTTAAAA..TTTTAAAAAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 1704 nt

>oriT_pCRE17_5
GCCGTATGAATGACATTTATTTCCGTCCAATCCATCGCAATCAATATTTTGAGGGAATGAAAGCATGATAAAGAAACTTAAAGTAATCATGTTGGCGGCAATAGTTACCGGATGTTCATCGCCACCGCCTCCCGTTCCTGTAGAGTGGGATAAAGCCGCAGAGCCATTAAATACCAGTTTACCTCAATGGAGTGACAATAACGTTATAGTCCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAGTTTCTACGATACAATATGGACTCCAGCAGTATTTTACGCAGTCGCTCATTCAACACGGATTGTCGTCACTGCACAATCCGGTACTGATTTCTTTAATGCCAAAAGCTGGCTTCGCCAGAATGGCGCAAAAGGAGTTATTGAATACCAGCCTGTCTTTAATTGTCTGACGTGCAGAGAAACCACCATTTATTTCTCTCGCCAATCTATTTAAGTAATACAATCTAATAAGGATTTATCATGAAAAAAACCGTTATTACGGCGGCTGTATTATGCCTGACCGCAGGAACGCCGATTTATTCATTTGCCGCTGACCCATGCGAAGTTGTTCTTTGCATGTACGGAAAAGCAACGGGTAATAGTGGTGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATCAACGCTTTTAAAAAACACCATCGTTTTAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGCGAATGTAAAGCCGCAGACCCTGAATCAATAAGCAAAATCCTTTCAAAATTTGGCAGAGTGAAAGGATAACTGACATATCCATTTTGACATGAGAGGTTCGAAATGATAAACGAAAATTTATTCATTAAGAATATACACTCAAAAAATCAGGACAGAATATCTGTAGCCCTGGTATATGACACGTTATCAAAAGAAGCCCACAGAGGTTGTGGGCTGTATTACGAAATATACGAAAGCTGTTTTATCGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAAGCTGACGCAAATAAATTAAGACGCTATGCCGAAAGCAAAGGTACGAAAATTGATGATGCGTCCTACAGTGAAGCGCTGGAAGCTGAAAGGGAGTGCAGGTCTGAAATATACCGCGAGCAGATGTAATCACACAAAACAAAAGTGTATTGTACAATACACACTAACAGGCTGCGGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTTCGAAGAAATTGTTAAGACGAAAAATAATGAAGTGGTACGCGGAAAGAAAATTCAATTTCGAAGAAATTGCTAATTTTCAGGAACCGCGCCCACCTGCGAACATTCGCCCACAGCGAATTGAGCAGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGTTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGTAATATAACCCGCCCCAGCGGGGGATATTACCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCGATTTTTTCGAGCTTGCGAGGAAAAATAGGCTAAACGCAGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCAAGACGGTGTGCCGCCTTTGACCTTGGGGGTTTTGGCTCGGCGTCCAGCCGGGACATTCCCCCCATGTGTCATGCAGTGAGGGCGTCCAGCCCGAACGTAATGGCACACGCCGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP

ID   928 GenBank   WP_032722798
Name   t4cp2_KIN65_RS31650_pCRE17_5 insolico UniProt ID   _
Length   578 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 578 a.a.        Molecular weight: 65666.94 Da        Isoelectric Point: 7.4566

>WP_032722798.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLLAGLTLPFIFHLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKSLDNNILFRVPLWIMTIIFTAAGLIFHIAARRLLAGEIDNLKHRMIKKSKLERNT
RTDVRKVKELLPDPIEYNPLDYIDLKKGVFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGITATVLL
YQAILLGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLRDLFNTDFVQSLREDVPAFCGELEEIALVNSINATKGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDSVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKVDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTSTELPRATKMFQVQVKKENIELLSFEYK
QATPEKNESQEHKPSINL

  Protein domains


Predicted by InterproScan.

Helicase HerA, central domain (DUF87)

(174-280)

TraM recognition site of TraD and TraG (TraG-D_C)

(399-490)

  Protein structure



No available structure.



ID   929 GenBank   WP_012457110
Name   t4cp2_KIN65_RS31715_pCRE17_5 insolico UniProt ID   _
Length   912 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 912 a.a.        Molecular weight: 103056.99 Da        Isoelectric Point: 7.4625

>WP_012457110.1 MULTISPECIES: VirB3 family type IV secretion system protein [Enterobacterales]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

  Protein domains


Predicted by InterproScan.

Type IV secretory pathway, VirB3-like protein (VirB3)

(3-79)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(269-470)

TraG P-loop domain (P-loop_TraG)

(535-813)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 18425..31590

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIN65_RS31610 (KIN65_31470) 13866..14360 - 495 WP_227502068 DUF4326 domain-containing protein -
KIN65_RS31615 (KIN65_31475) 14429..14881 - 453 WP_032722770 DUF488 domain-containing protein -
KIN65_RS31620 (KIN65_31480) 14882..15529 - 648 WP_045286760 hypothetical protein -
KIN65_RS31625 (KIN65_31485) 15790..16437 + 648 WP_004178130 ParA family protein -
KIN65_RS31630 (KIN65_31490) 16478..16711 + 234 WP_004178129 hypothetical protein -
KIN65_RS31635 (KIN65_31495) 16749..17090 - 342 WP_032722766 hypothetical protein -
KIN65_RS31640 (KIN65_31500) 17151..17624 - 474 WP_032722765 thermonuclease family protein -
KIN65_RS31645 (KIN65_31505) 17661..18416 - 756 WP_023316386 MobC family replication-relaxation protein -
KIN65_RS31650 (KIN65_31510) 18425..20161 - 1737 WP_032722798 type IV secretion system DNA-binding domain-containing protein virb4
KIN65_RS31655 (KIN65_31515) 20939..21244 - 306 WP_045286759 hypothetical protein -
KIN65_RS31660 (KIN65_31520) 21278..21583 - 306 WP_032722794 conjugal transfer protein -
KIN65_RS31665 (KIN65_31525) 21610..22017 - 408 WP_071993161 cag pathogenicity island Cag12 family protein -
KIN65_RS31670 (KIN65_31530) 22014..23039 - 1026 WP_268014069 P-type DNA transfer ATPase VirB11 virB11
KIN65_RS31675 (KIN65_31535) 23029..23349 - 321 WP_032722553 hypothetical protein -
KIN65_RS31680 (KIN65_31540) 23361..24623 - 1263 WP_032722790 VirB10/TraB/TrbI family type IV secretion system protein virB10
KIN65_RS31685 (KIN65_31545) 24670..25578 - 909 WP_087749168 P-type conjugative transfer protein VirB9 virB9
KIN65_RS31690 (KIN65_31550) 25575..26258 - 684 WP_004178111 type IV secretion system protein virB8
KIN65_RS31695 (KIN65_31555) 26251..26388 - 138 WP_039109361 hypothetical protein -
KIN65_RS31700 (KIN65_31560) 26480..27556 - 1077 WP_004178110 type IV secretion system protein virB6
KIN65_RS31705 (KIN65_31565) 27568..27795 - 228 WP_032722788 EexN family lipoprotein -
KIN65_RS31710 (KIN65_31570) 27805..28515 - 711 WP_012457111 type IV secretion system protein -
KIN65_RS31715 (KIN65_31575) 28534..31272 - 2739 WP_012457110 VirB3 family type IV secretion system protein virb4
KIN65_RS31720 (KIN65_31580) 31285..31590 - 306 WP_012457109 TrbC/VirB2 family protein virB2
KIN65_RS31725 (KIN65_31585) 31587..31808 - 222 Protein_40 type VI secretion protein -
KIN65_RS31730 (KIN65_31590) 31861..33576 - 1716 WP_004152391 Tn3-like element Tn4401 family resolvase TnpR -

Host bacterium

ID   1900 GenBank   NZ_JAHBNO010000007
Plasmid name   pCRE17_5 Incompatibility group   IncFII
Plasmid size   37843 bp Coordinate of oriT [Strand]   20378..22081 [-]
Host baterium   Klebsiella michiganensis strain S17_CRE17

Cargo genes

Drug resistance gene   blaKPC-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -