Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 101437
• Name: oriT_FWSEC0021|unnamed3
• Organism: Escherichia coli strain FWSEC0021
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_RRCV01000192 (4474..4565 [-], 92 nt)
• oriT length: 92 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 92 nt

>oriT_FWSEC0021|unnamed3
CGGGGTGTCGGGGCTAAGCCCTGACCAGGTGGTAATCGTATAGCCGTGCGTGCGCGGTTATACGATTACACATCCTGTCCCGTTTTTCAGGC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_C9Y74_RS26060_FWSEC0021|unnamed3

• ID: 1302
• GenBank: WP_000991399
• Name: Relaxase_C9Y74_RS26060_FWSEC0021|unnamed3
• UniProt ID: A0AA35F5B1
• Length: 906 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 906 a.a.
• Molecular weight: 105033.78 Da
• Isoelectric Point: 8.0416

>WP_000991399.1 relaxase/mobilization nuclease domain-containing protein [Escherichia coli]
MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEIPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCLSLESAAEEMQKAADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELEIKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIVQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGVALSSFGPLWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRHFAERLPEMARNGELESCLDVHRTLATAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGRNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
GLIQCRSLIEEMIADGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQLMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRTTNVDRCVVLCEPGGTPLFNNVAKLEARLQK
NGSVHFRDTRTGKNVCTDYGDRVVFYHHTDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM
VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSVSHDDNYTLRQEKTWEPPSPGM

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (62-315)

Protein structure

No available structure.

Auxiliary protein

WP_001291056_FWSEC0021|unnamed3

• ID: 462
• GenBank: WP_001291056
• Name: WP_001291056_FWSEC0021|unnamed3
• UniProt ID: A0A4P8CE48
• Length: 110 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 110 a.a.
• Molecular weight: 12597.68 Da
• Isoelectric Point: 10.6096

>WP_001291056.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVSTLIRKTVLGKQINARIDEDFLKELMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITELANTLRRDLMGR

Protein domains

Predicted by InterproScan.

• Family of unknown function (DUF6290) (DUF6290) (19-66)

Protein structure

• AlphaFold DB: A0A4P8CE48

Host bacterium

• ID: 1881
• GenBank: NZ_RRCV01000192
• Plasmid name: FWSEC0021|unnamed3
• Incompatibility group: -
• Plasmid size: 8237 bp
• Coordinate of oriT [Strand]: 4474..4565 [-]
• Host baterium: Escherichia coli strain FWSEC0021

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -