Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 101420
• Name: oriT_pAsa4
• Organism: Aeromonas salmonicida subsp. salmonicida strain RS534
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_JYFF01000051 (16803..16932 [+], 130 nt)
• oriT length: 130 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 130 nt

>oriT_pAsa4
GTTAACCCGTTTGATCGCGTGATGAATGCGCTAAAAAGTCGAGGCCGTAAGAACGCCCACATTCTGAGCATCTTGCAGCTGGATTGGCCTGCGTCTGAATCGATCATCCATCGCCTGAGCGACTACATCA

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobH_UC37_RS20775_pAsa4

• ID: 1292
• GenBank: WP_236614860
• Name: mobH_UC37_RS20775_pAsa4
• UniProt ID: _
• Length: 984 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 984 a.a.
• Molecular weight: 107305.59 Da
• Isoelectric Point: 4.7871

>WP_236614860.1 MobH family relaxase [Aeromonas salmonicida]
MDEEDIPRYPPFAKGLPVAPIDKILATQAELIEKVRNSLGFTIEDFNRLVLPVIHRYAAFVHLLPASEAH
HHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPKQRRDNEPRWRLASCFSGLLHDVGKPLADVSITDKN
GVVTWNPYFESLHDWAGRNHVDRYFIRWREKRHKRHEQFSLLAVDRIIPVATREYLSESGPLIIEAMLEA
ISGTSVNQPVTKLMLRADQESVSRDLKQNRLNVDEFSYGVPVERYVFDAIRRLVKTGKWKVNEPGAKVWH
LQQGVFIAWRNLGDLYDLISHDKIPGIPRDPDTLADILIERGFAVPNTVQEKGDRAYYRYWEVLPEMIQE
AAGSIKILMLRLESNELVFTTEPPAAVAGEVVGDVADAVIELIDPDEASDADDESASGMSESLLSAEQEA
MNALAGLGFGDAMEILGGTASDDNATQPAPVVDTTPPAKPAKGAGGRGGKNQAKKTAPAAPEQQQPEPSP
QEIAKSTPPLAVENPLQAIMDVGGGLGGLDFPFDAFVAGTSMSDEPSQALAPEPKQDVANPDNPDTPNLG
LMNQVFPNFMNDESTGDVGGMDLPPAWAIEEIPSFHAYEGEVGQPGNQFVSEELAGADAPDAKGELLTLM
ATFGEAGPILERAILPVLEGKATLGEVLCLMKGQAVILYPEGARALGAPSEILTALFHAQAIVPDPVMSG
RKVQDFNGVKAIVLTEQLSSAFIAAIKQAESLLGGYQDAFELVASPEVKKTQSKKNRGQRKTSRSESSGE
DSHAASPERAQVEEKRVIADAQLIDVSVEPPSPPVDTTPPPDKVVPRKESRGKEKVDKPIQPAPTTKPTE
GTQNLARLPPREQPKPESQQKQARVIDVGNIALPGPDLPEELSPRSIVKEETVDEALYLPPKMTPEKAIQ
MLKEMIKNRSGRWLVSAVLEEEGFLTTSDKAFEVIAGEYTGISKHVLGGTLRRAQRRPLLKQRQGKLYLE
VDKS

Protein domains

Predicted by InterproScan.

• Putative helicase (TraI_2) (22-328)

Protein structure

No available structure.

T4CP

traD_UC37_RS20780_pAsa4

• ID: 905
• GenBank: WP_011899407
• Name: traD_UC37_RS20780_pAsa4
• UniProt ID: _
• Length: 621 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 621 a.a.
• Molecular weight: 68931.34 Da
• Isoelectric Point: 6.8339

>WP_011899407.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTYDPLAYEMPWRPNYEMSAVFGWVAASVGALAVQQITDMPPEPFYWMTGICGVMTMARLPKAIKLHLLQ
KHLKGRDLEFISIKELQKKVQASPEDMWLGYGFAWENRHAQRVFEILKRDWTSIVGTESTTSKIVRTIRG
KKKKEVPIGQPWIHGVEPKEEHLMQPLKHTEGHSLIVGTTGSGKTRMFDTLISQAILRGEAVIIIDPKGD
KEMRDNARRACEAMGQAERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEGGGDPFKSFGWQA
LNNIAQGLILAYDRPNLIKLRRFLEGGADGLVIKAVQAYAERVMPDWEAAAAPYLEKAANGSRSKLAVAH
MAFYNNVIQPEHPSSELEGLLNMFKHDATHFSKMVSNLLPIMNMLTSGELGPLLSPDATDLSDERQITDS
AKIINNAQVAYLGLDSLTDNMVGSALGSIFLSDLTAVAGDRYNYGVNNLPVNIFVDEAAEVINDPFIQLL
NKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNVFALRIVDAETQEYIAENLPKTRLKYVMRTQ
GQNTDGSEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGKIVKGRLPILTEK

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (470-597)

• AAA-like domain (AAA_10) (168-257)

Protein structure

No available structure.

Host bacterium

• ID: 1864
• GenBank: NZ_JYFF01000051
• Plasmid name: pAsa4
• Incompatibility group: -
• Plasmid size: 18888 bp
• Coordinate of oriT [Strand]: 16803..16932 [+]
• Host baterium: Aeromonas salmonicida subsp. salmonicida strain RS534

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -