Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101419
Name   oriT_pB-9428-5 in_silico
Organism   Escherichia coli strain SCPM-O-B-9428
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAJIZO010000012 (522..696 [-], 175 nt)
oriT length   175 nt
IRs (inverted repeats)      101..106, 120..125  (ACAATC..GATTGT)
Location of nic site      65..66
Conserved sequence flanking the
  nic site    		  ACTGGCATAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 175 nt

>oriT_pB-9428-5
GTGACTCCGTGCGCGGTGAAAAATCGCATTTTAGCGCGTCACTGGTAGTTGCAAAACTGAACTGGCATAATGCACGGCACATCACGAAGTGCGCACTTATACAATCTCTACTCCGTTTCGATTGTGTGCGGTCTGCGACGCTAAAAGAAAACGGCAAAAAGGTATTACGGCAGAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1291 GenBank   WP_052979306
Name   mobQ_LMH83_RS26900_pB-9428-5 insolico UniProt ID   A0A0G3EZY5
Length   739 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 739 a.a.        Molecular weight: 84368.62 Da        Isoelectric Point: 8.2796

>WP_052979306.1 MULTISPECIES: MobQ family relaxase [Enterobacteriaceae]
MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRKRGVDDAVILTPANAPSWCSDRSVLW
NAVEKAEQRRNSQLAREIELAIPREISREAAREAVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTT
RAVGETGFAGKVRDWNERALAETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAM
EKRGMETERGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLR
EFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASLLSEIPLMTWA
EPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIAPGMAELKEKLSAREAERRREE
AEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAH
YPQSVHDPVNVYLSPGGAVMVSDIREGMPESHETMARPERMRMYSGATVRHVLEQMRQGWPSYGFPALPH
HWPDNFYFSDDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREG
GFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAAVHKPEE
MPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(18-213)


  Protein structure


Source ID Structure
AlphaFold DB A0A0G3EZY5


Host bacterium

ID   1863 GenBank   NZ_JAJIZO010000012
Plasmid name   pB-9428-5 Incompatibility group   Col156
Plasmid size   6728 bp Coordinate of oriT [Strand]   522..696 [-]
Host baterium   Escherichia coli strain SCPM-O-B-9428

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -