Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101412
Name   oriT_pP16C in_silico
Organism   Klebsiella pneumoniae strain P16
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JABENA010000005 (29997..30099 [+], 103 nt)
oriT length   103 nt
IRs (inverted repeats)      30..35, 38..43  (GCCGGC..GCCGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 103 nt

>oriT_pP16C
TACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1288 GenBank   WP_141416535
Name   traI_HKJ74_RS26445_pP16C insolico UniProt ID   _
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75204.95 Da        Isoelectric Point: 9.9948

>WP_141416535.1 TraI/MobA(P) family conjugative relaxase [Klebsiella pneumoniae]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLIRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQNTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMDERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(87-334)


  Protein structure



No available structure.




T4CP

ID   901 GenBank   WP_004187443
Name   t4cp2_HKJ74_RS26230_pP16C insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79519.87 Da        Isoelectric Point: 4.8309

>WP_004187443.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 32897..52007

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKJ74_RS26425 (HKJ74_26955) 27982..28293 + 312 WP_141416534 hypothetical protein -
HKJ74_RS26430 (HKJ74_26960) 28426..28962 + 537 WP_015062822 hypothetical protein -
HKJ74_RS26435 (HKJ74_26965) 29463..29858 - 396 WP_019725163 hypothetical protein -
HKJ74_RS26440 (HKJ74_26970) 30103..30420 + 318 WP_004206923 plasmid mobilization protein MobA -
HKJ74_RS26445 (HKJ74_26975) 30407..32386 + 1980 WP_141416535 TraI/MobA(P) family conjugative relaxase -
HKJ74_RS26450 (HKJ74_26980) 32400..32900 + 501 WP_004187320 DotD/TraH family lipoprotein -
HKJ74_RS26455 (HKJ74_26985) 32897..33676 + 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
HKJ74_RS26460 (HKJ74_26990) 33687..34850 + 1164 WP_015062827 plasmid transfer ATPase TraJ virB11
HKJ74_RS26465 (HKJ74_26995) 34840..35100 + 261 WP_004187310 IcmT/TraK family protein traK
HKJ74_RS26680 35125..38382 + 3258 WP_223135410 LPD7 domain-containing protein -
HKJ74_RS26475 (HKJ74_27005) 38348..38860 + 513 WP_011091071 hypothetical protein traL
HKJ74_RS26480 (HKJ74_27010) 38861..39073 - 213 WP_305953721 Hha/YmoA family nucleoid-associated regulatory protein -
HKJ74_RS26485 (HKJ74_27015) 39045..39455 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
HKJ74_RS26490 (HKJ74_27020) 39523..40236 + 714 WP_228298261 DotI/IcmL family type IV secretion protein traM
HKJ74_RS26495 (HKJ74_27025) 40245..41396 + 1152 WP_011091074 DotH/IcmK family type IV secretion protein traN
HKJ74_RS26500 (HKJ74_27030) 41408..42757 + 1350 WP_015062830 conjugal transfer protein TraO traO
HKJ74_RS26505 (HKJ74_27035) 42769..43329 + 561 WP_228727566 TraP traP
HKJ74_RS26510 (HKJ74_27040) 43498..44028 + 531 WP_011091077 conjugal transfer protein TraQ traQ
HKJ74_RS26515 (HKJ74_27045) 44045..44434 + 390 WP_015062832 DUF6750 family protein traR
HKJ74_RS26520 (HKJ74_27050) 44480..44974 + 495 WP_004187480 hypothetical protein -
HKJ74_RS26525 (HKJ74_27055) 44971..48021 + 3051 WP_040119746 conjugal transfer protein traU
HKJ74_RS26530 (HKJ74_27060) 48018..49226 + 1209 WP_141416537 conjugal transfer protein TraW traW
HKJ74_RS26535 (HKJ74_27065) 49223..49819 + 597 WP_015060003 hypothetical protein -
HKJ74_RS26540 (HKJ74_27070) 49812..52007 + 2196 WP_015062834 DotA/TraY family protein traY
HKJ74_RS26545 (HKJ74_27075) 52009..52662 + 654 WP_015062835 hypothetical protein -
HKJ74_RS26550 (HKJ74_27080) 52726..52974 + 249 WP_015062836 IncL/M type plasmid replication protein RepC -

Host bacterium

ID   1856 GenBank   NZ_JABENA010000005
Plasmid name   pP16C Incompatibility group   IncL/M
Plasmid size   53271 bp Coordinate of oriT [Strand]   29997..30099 [+]
Host baterium   Klebsiella pneumoniae strain P16

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -