Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101399 |
| Name | oriT_CPKp171210|unnamed1  |
| Organism | Klebsiella pneumoniae strain CPKp171210 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_WMHR01000003 (93783..93831 [-], 49 nt) |
| oriT length | 49 nt |
| IRs (inverted repeats) | 6..13, 16..23 (GCAAAATT..AATTTTGC) |
| Location of nic site | 32..33 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 49 nt
>oriT_CPKp171210|unnamed1
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 892 | GenBank | WP_117251425 |
| Name | traD_F8B39_RS26830_CPKp171210|unnamed1 |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 85943.03 Da Isoelectric Point: 5.1183
>WP_117251425.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPEPGPADTDSHAGEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPEPGPADTDSHAGEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1635..21386
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| F8B39_RS26715 | 6..317 | + | 312 | WP_029497417 | hypothetical protein | - |
| F8B39_RS26720 | 384..788 | + | 405 | WP_004197817 | hypothetical protein | - |
| F8B39_RS29045 (F8B39_05277) | 831..983 | + | 153 | WP_224518895 | hypothetical protein | - |
| F8B39_RS26730 (F8B39_05278) | 1165..1563 | + | 399 | WP_023179972 | hypothetical protein | - |
| F8B39_RS26735 (F8B39_05279) | 1635..4274 | + | 2640 | WP_013214024 | type IV secretion system protein TraC | virb4 |
| F8B39_RS26740 (F8B39_05280) | 4274..4663 | + | 390 | WP_064291687 | type-F conjugative transfer system protein TrbI | - |
| F8B39_RS26745 (F8B39_05281) | 4663..5298 | + | 636 | WP_020325113 | type-F conjugative transfer system protein TraW | traW |
| F8B39_RS26750 (F8B39_05282) | 5333..5734 | + | 402 | WP_004194979 | hypothetical protein | - |
| F8B39_RS26755 (F8B39_05283) | 5731..6720 | + | 990 | WP_029497420 | conjugal transfer pilus assembly protein TraU | traU |
| F8B39_RS26760 (F8B39_05284) | 6733..7371 | + | 639 | WP_015065635 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| F8B39_RS26765 (F8B39_05285) | 7430..9385 | + | 1956 | WP_101455617 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| F8B39_RS26770 (F8B39_05286) | 9417..9671 | + | 255 | WP_004152674 | conjugal transfer protein TrbE | - |
| F8B39_RS26775 (F8B39_05287) | 9649..9897 | + | 249 | WP_004152675 | hypothetical protein | - |
| F8B39_RS26780 (F8B39_05288) | 9910..10236 | + | 327 | WP_004144402 | hypothetical protein | - |
| F8B39_RS26785 (F8B39_05289) | 10257..11009 | + | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| F8B39_RS26790 (F8B39_05290) | 11020..11259 | + | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| F8B39_RS26795 (F8B39_05291) | 11231..11788 | + | 558 | WP_013214031 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| F8B39_RS26800 (F8B39_05292) | 11834..12277 | + | 444 | WP_101455614 | F-type conjugal transfer protein TrbF | - |
| F8B39_RS26805 (F8B39_05293) | 12255..13634 | + | 1380 | WP_072198320 | conjugal transfer pilus assembly protein TraH | traH |
| F8B39_RS26810 (F8B39_05294) | 13634..16456 | + | 2823 | WP_101455613 | conjugal transfer mating-pair stabilization protein TraG | traG |
| F8B39_RS26815 (F8B39_05295) | 16479..17081 | + | 603 | WP_013214035 | conjugal transfer protein TraS | - |
| F8B39_RS26820 (F8B39_05296) | 17332..18063 | + | 732 | WP_016831056 | conjugal transfer complement resistance protein TraT | - |
| F8B39_RS28660 (F8B39_05297) | 18256..18945 | + | 690 | WP_162375242 | hypothetical protein | - |
| F8B39_RS26830 (F8B39_05298) | 19074..21386 | + | 2313 | WP_117251425 | type IV conjugative transfer system coupling protein TraD | virb4 |
Region 2: 93225..99899
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| F8B39_RS27225 (F8B39_05375) | 88327..88677 | + | 351 | WP_004153414 | hypothetical protein | - |
| F8B39_RS27230 (F8B39_05376) | 89307..89660 | + | 354 | WP_004152748 | hypothetical protein | - |
| F8B39_RS27235 (F8B39_05377) | 89717..90064 | + | 348 | WP_004152749 | hypothetical protein | - |
| F8B39_RS27240 (F8B39_05378) | 90159..90305 | + | 147 | WP_004152750 | hypothetical protein | - |
| F8B39_RS27245 (F8B39_05379) | 90356..91189 | + | 834 | WP_004152751 | N-6 DNA methylase | - |
| F8B39_RS27250 (F8B39_05380) | 92009..92830 | + | 822 | WP_004152492 | DUF932 domain-containing protein | - |
| F8B39_RS27255 (F8B39_05381) | 92863..93192 | + | 330 | WP_011977736 | DUF5983 family protein | - |
| F8B39_RS27260 (F8B39_05382) | 93225..93710 | - | 486 | WP_001568108 | transglycosylase SLT domain-containing protein | virB1 |
| F8B39_RS27265 (F8B39_05383) | 94142..94534 | + | 393 | WP_004194114 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| F8B39_RS27270 (F8B39_05384) | 94764..95465 | + | 702 | WP_004194113 | hypothetical protein | - |
| F8B39_RS27275 | 95550..95750 | + | 201 | WP_046664192 | TraY domain-containing protein | - |
| F8B39_RS27280 (F8B39_05385) | 95818..96186 | + | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| F8B39_RS27285 (F8B39_05386) | 96200..96505 | + | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| F8B39_RS27290 (F8B39_05387) | 96525..97091 | + | 567 | WP_064161971 | type IV conjugative transfer system protein TraE | traE |
| F8B39_RS27295 (F8B39_05388) | 97078..97818 | + | 741 | WP_064169777 | type-F conjugative transfer system secretin TraK | traK |
| F8B39_RS27300 (F8B39_05389) | 97818..99242 | + | 1425 | WP_040228261 | F-type conjugal transfer pilus assembly protein TraB | traB |
| F8B39_RS27305 (F8B39_05390) | 99315..99899 | + | 585 | WP_040120377 | type IV conjugative transfer system lipoprotein TraV | traV |
Host bacterium
| ID | 1843 | GenBank | NZ_WMHR01000003 |
| Plasmid name | CPKp171210|unnamed1 | Incompatibility group | IncFII |
| Plasmid size | 100435 bp | Coordinate of oriT [Strand] | 93783..93831 [-] |
| Host baterium | Klebsiella pneumoniae strain CPKp171210 |
Cargo genes
| Drug resistance gene | qnrS1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |