Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101397
Name   oriT_CPKp1825|unnamed1 in_silico
Organism   Klebsiella pneumoniae strain CPKp1825
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_WMHT01000002 (175389..175438 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_CPKp1825|unnamed1
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1279 GenBank   WP_015065542
Name   traI_F8B41_RS27385_CPKp1825|unnamed1 insolico UniProt ID   K7R3U7
Length   1752 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1752 a.a.        Molecular weight: 190318.03 Da        Isoelectric Point: 6.2890

>WP_015065542.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMSREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALAVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAAGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDTAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGGGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD

  Protein domains


Predicted by InterproScan.

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(576-628)

DNA helicase TraI (TraI_C)

(1432-1554)

TrwC relaxase (TrwC)

(11-283)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(634-713)

AAA domain (AAA_30)

(970-1156)


  Protein structure



No available structure.




T4CP

ID   890 GenBank   WP_015065541
Name   traD_F8B41_RS27390_CPKp1825|unnamed1 insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 86025.00 Da        Isoelectric Point: 5.0577

>WP_015065541.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 145982..175996

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F8B41_RS27390 (F8B41_05408) 145982..148294 - 2313 WP_015065541 type IV conjugative transfer system coupling protein TraD virb4
F8B41_RS29170 (F8B41_05409) 148423..149112 - 690 WP_015065540 hypothetical protein -
F8B41_RS27400 (F8B41_05410) 149304..150035 - 732 WP_032329926 conjugal transfer complement resistance protein TraT -
F8B41_RS27405 (F8B41_05411) 150344..150868 - 525 WP_015065538 hypothetical protein -
F8B41_RS27410 (F8B41_05412) 150879..153722 - 2844 WP_023157999 conjugal transfer mating-pair stabilization protein TraG traG
F8B41_RS27415 (F8B41_05413) 153722..155101 - 1380 WP_023158000 conjugal transfer pilus assembly protein TraH traH
F8B41_RS27420 (F8B41_05414) 155079..155522 - 444 WP_023158001 F-type conjugal transfer protein TrbF -
F8B41_RS27425 (F8B41_05415) 155568..156125 - 558 WP_023158002 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
F8B41_RS27430 (F8B41_05416) 156097..156336 - 240 WP_023158003 type-F conjugative transfer system pilin chaperone TraQ -
F8B41_RS27435 (F8B41_05417) 156347..157099 - 753 WP_015065535 type-F conjugative transfer system pilin assembly protein TraF traF
F8B41_RS27440 (F8B41_05418) 157120..157446 - 327 WP_015065534 hypothetical protein -
F8B41_RS27445 (F8B41_05419) 157459..157707 - 249 WP_004152675 hypothetical protein -
F8B41_RS27450 (F8B41_05420) 157685..157939 - 255 WP_015065558 conjugal transfer protein TrbE -
F8B41_RS27455 (F8B41_05421) 157971..159926 - 1956 WP_015065533 type-F conjugative transfer system mating-pair stabilization protein TraN traN
F8B41_RS27460 (F8B41_05422) 159985..160632 - 648 WP_015632510 type-F conjugative transfer system pilin assembly protein TrbC trbC
F8B41_RS29310 (F8B41_05423) 160711..160887 - 177 WP_162866177 hypothetical protein -
F8B41_RS27465 (F8B41_05424) 160908..161897 - 990 WP_015632509 conjugal transfer pilus assembly protein TraU traU
F8B41_RS27470 (F8B41_05425) 161894..162295 - 402 WP_023158005 hypothetical protein -
F8B41_RS27475 (F8B41_05426) 162330..162965 - 636 WP_023158006 type-F conjugative transfer system protein TraW traW
F8B41_RS27480 (F8B41_05427) 162965..163354 - 390 WP_004167468 type-F conjugative transfer system protein TrbI -
F8B41_RS27485 (F8B41_05428) 163354..165993 - 2640 WP_023158007 type IV secretion system protein TraC virb4
F8B41_RS27490 (F8B41_05429) 166065..166463 - 399 WP_023158008 hypothetical protein -
F8B41_RS29315 (F8B41_05430) 166471..166860 - 390 WP_023158009 hypothetical protein -
F8B41_RS27500 (F8B41_05431) 166903..167307 - 405 WP_016831042 hypothetical protein -
F8B41_RS27505 (F8B41_05432) 167374..167685 - 312 WP_014386200 hypothetical protein -
F8B41_RS27510 (F8B41_05433) 167686..167904 - 219 WP_014386199 hypothetical protein -
F8B41_RS27515 (F8B41_05434) 167928..168217 - 290 Protein_174 hypothetical protein -
F8B41_RS27520 (F8B41_05435) 168222..168632 - 411 WP_016831039 hypothetical protein -
F8B41_RS27525 (F8B41_05436) 168764..169348 - 585 WP_014386196 type IV conjugative transfer system lipoprotein TraV traV
F8B41_RS27530 169371..169967 - 597 Protein_177 conjugal transfer pilus-stabilizing protein TraP -
F8B41_RS27535 (F8B41_05439) 169960..171384 - 1425 WP_023158013 F-type conjugal transfer pilus assembly protein TraB traB
F8B41_RS27540 (F8B41_05440) 171384..172124 - 741 WP_014386193 type-F conjugative transfer system secretin TraK traK
F8B41_RS27545 (F8B41_05441) 172111..172677 - 567 WP_016831035 type IV conjugative transfer system protein TraE traE
F8B41_RS27550 (F8B41_05442) 172697..173002 - 306 WP_004144424 type IV conjugative transfer system protein TraL traL
F8B41_RS27555 (F8B41_05443) 173016..173384 - 369 WP_004194426 type IV conjugative transfer system pilin TraA -
F8B41_RS27560 173446..173652 - 207 WP_171773970 TraY domain-containing protein -
F8B41_RS27565 (F8B41_05444) 173785..174504 - 720 WP_016831034 conjugal transfer protein TrbJ -
F8B41_RS27570 (F8B41_05445) 174704..175120 - 417 WP_072145360 conjugal transfer relaxosome DNA-binding protein TraM -
F8B41_RS27575 (F8B41_05446) 175511..175996 + 486 WP_004178063 transglycosylase SLT domain-containing protein virB1
F8B41_RS27580 (F8B41_05447) 176029..176358 - 330 WP_011977736 DUF5983 family protein -
F8B41_RS27585 (F8B41_05448) 176391..177212 - 822 WP_004182076 DUF932 domain-containing protein -
F8B41_RS27590 (F8B41_05450) 178040..178603 - 564 WP_004182054 methyltransferase -
F8B41_RS27595 (F8B41_05451) 178651..180006 - 1356 WP_004182052 DUF3560 domain-containing protein -
F8B41_RS27600 (F8B41_05452) 180058..180288 - 231 WP_001568051 hypothetical protein -
F8B41_RS27605 (F8B41_05453) 180380..180607 - 228 WP_004182050 hypothetical protein -

Host bacterium

ID   1841 GenBank   NZ_WMHT01000002
Plasmid name   CPKp1825|unnamed1 Incompatibility group   IncFIB
Plasmid size   198487 bp Coordinate of oriT [Strand]   175389..175438 [+]
Host baterium   Klebsiella pneumoniae strain CPKp1825

Cargo genes

Drug resistance gene   aac(6')-Ib-cr, blaOXA-1
Virulence gene   -
Metal resistance gene   silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsH, arsC, arsB, arsA, arsD, arsR, fecD, fecE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -