Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101385
Name   oriT_FDAARGOS_626|unnamed1 in_silico
Organism   Klebsiella quasipneumoniae strain FDAARGOS_626
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAAVWJ010000002 (20218..20318 [-], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_FDAARGOS_626|unnamed1
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1269 GenBank   WP_167874753
Name   mobF_FOB34_RS25835_FDAARGOS_626|unnamed1 insolico UniProt ID   _
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120236.47 Da        Isoelectric Point: 6.6314

>WP_167874753.1 MobF family relaxase [Klebsiella quasipneumoniae]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSRDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure



No available structure.




Auxiliary protein

ID   452 GenBank   WP_001749975
Name   WP_001749975_FDAARGOS_626|unnamed1 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   880 GenBank   WP_000342688
Name   t4cp2_FOB34_RS25830_FDAARGOS_626|unnamed1 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 20885..37307

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB34_RS25800 (FOB34_25805) 17221..17400 + 180 WP_012561134 protein CcgAI -
FOB34_RS25805 (FOB34_25810) 17862..18206 - 345 WP_012561137 hypothetical protein -
FOB34_RS25810 (FOB34_25815) 18407..18775 - 369 WP_012561138 plasmid stabilization protein StbC -
FOB34_RS25815 (FOB34_25820) 18777..19493 - 717 WP_012561139 StbB family protein -
FOB34_RS25820 (FOB34_25825) 19502..19888 - 387 WP_013279398 plasmid stabilization protein StbA -
FOB34_RS25825 (FOB34_25830) 20467..20883 + 417 WP_001749975 hypothetical protein -
FOB34_RS25830 (FOB34_25835) 20885..22414 + 1530 WP_000342688 type IV secretion system DNA-binding domain-containing protein virb4
FOB34_RS25835 (FOB34_25840) 22414..25650 + 3237 WP_167874753 MobF family relaxase -
FOB34_RS27975 25650..26276 + 627 WP_049067176 DUF6710 family protein -
FOB34_RS25850 (FOB34_25855) 26449..26982 - 534 WP_000792636 phospholipase D family protein -
FOB34_RS25855 (FOB34_25860) 26982..27977 - 996 WP_012561144 ATPase, T2SS/T4P/T4SS family virB11
FOB34_RS25860 (FOB34_25865) 28019..29179 - 1161 WP_042098201 type IV secretion system protein VirB10 virB10
FOB34_RS25865 (FOB34_25870) 29179..30063 - 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
FOB34_RS25870 (FOB34_25875) 30074..30772 - 699 WP_000646594 type IV secretion system protein virB8
FOB34_RS27980 30762..30920 - 159 WP_012561180 hypothetical protein -
FOB34_RS25875 (FOB34_25880) 30991..32031 - 1041 WP_001749958 type IV secretion system protein virB6
FOB34_RS25880 (FOB34_25885) 32047..32274 - 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
FOB34_RS25885 (FOB34_25890) 32282..32995 - 714 WP_001749960 type IV secretion system protein virB5
FOB34_RS25890 (FOB34_25895) 33013..35613 - 2601 WP_042098203 VirB4 family type IV secretion/conjugal transfer ATPase virb4
FOB34_RS25895 (FOB34_25900) 35613..35930 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
FOB34_RS25900 (FOB34_25905) 35980..36273 - 294 WP_001749962 hypothetical protein virB2
FOB34_RS25905 (FOB34_25910) 36283..36564 - 282 WP_032335136 transcriptional repressor KorA -
FOB34_RS25910 (FOB34_25915) 36573..37307 - 735 WP_032335135 lytic transglycosylase domain-containing protein virB1
FOB34_RS25915 (FOB34_25920) 37416..37721 + 306 WP_000960954 H-NS family nucleoid-associated regulatory protein -
FOB34_RS25920 (FOB34_25925) 37737..38081 + 345 WP_042098207 hypothetical protein -
FOB34_RS25925 (FOB34_25930) 38078..38392 + 315 WP_000734776 TrbM/KikA/MpfK family conjugal transfer protein -
FOB34_RS25930 (FOB34_25935) 38506..38739 + 234 WP_001191790 hypothetical protein -
FOB34_RS25935 (FOB34_25940) 38789..39436 + 648 WP_228284205 restriction endonuclease -
FOB34_RS25940 (FOB34_25945) 39441..39614 + 174 WP_167874754 hypothetical protein -
FOB34_RS25945 (FOB34_25950) 39660..40628 - 969 WP_013815099 IS5-like element IS903B family transposase -
FOB34_RS25950 (FOB34_25955) 40969..42294 - 1326 WP_049258899 alpha-amylase family glycosyl hydrolase -

Host bacterium

ID   1829 GenBank   NZ_JAAVWJ010000002
Plasmid name   FDAARGOS_626|unnamed1 Incompatibility group   IncN
Plasmid size   46054 bp Coordinate of oriT [Strand]   20218..20318 [-]
Host baterium   Klebsiella quasipneumoniae strain FDAARGOS_626

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -