Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 101384 |
Name | oriT_pK012_2 |
Organism | Klebsiella pneumoniae strain 12 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_RSEZ01000005 (85487..85536 [-], 50 nt) |
oriT length | 50 nt |
IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
Location of nic site | 33..34 |
Conserved sequence flanking the nic site |
TGTGTGGTGA |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pK012_2
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 1268 | GenBank | WP_065519309 |
Name | traI_EJB31_RS25545_pK012_2 | UniProt ID | A0A809SXI2 |
Length | 1752 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 190492.21 Da Isoelectric Point: 6.0263
>WP_065519309.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWLTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGDRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAPGRGNDLLISRQAWDAEKSILTRVLEGKDAVAPLMDRVPDSLMTELTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWLTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGDRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAPGRGNDLLISRQAWDAEKSILTRVLEGKDAVAPLMDRVPDSLMTELTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
ID | 879 | GenBank | WP_013214038 |
Name | traD_EJB31_RS25540_pK012_2 | UniProt ID | _ |
Length | 770 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 86010.07 Da Isoelectric Point: 5.1787
>WP_013214038.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 84929..113541
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
EJB31_RS25295 (EJB31_25375) | 80083..80400 | + | 318 | WP_014343505 | hypothetical protein | - |
EJB31_RS25315 (EJB31_25385) | 81241..81597 | + | 357 | WP_014343501 | hypothetical protein | - |
EJB31_RS25320 (EJB31_25390) | 81658..81870 | + | 213 | WP_014343500 | hypothetical protein | - |
EJB31_RS25325 (EJB31_25395) | 81881..82105 | + | 225 | WP_014343499 | hypothetical protein | - |
EJB31_RS25330 (EJB31_25400) | 82186..82506 | + | 321 | WP_014343498 | type II toxin-antitoxin system RelE/ParE family toxin | - |
EJB31_RS25335 (EJB31_25405) | 82496..82774 | + | 279 | WP_004152721 | helix-turn-helix transcriptional regulator | - |
EJB31_RS26315 (EJB31_25410) | 82778..82882 | + | 105 | Protein_99 | transcriptional regulator | - |
EJB31_RS25355 (EJB31_25425) | 83713..84534 | + | 822 | WP_004152492 | DUF932 domain-containing protein | - |
EJB31_RS25360 (EJB31_25430) | 84567..84896 | + | 330 | WP_013214015 | DUF5983 family protein | - |
EJB31_RS25365 (EJB31_25435) | 84929..85414 | - | 486 | WP_014343495 | transglycosylase SLT domain-containing protein | virB1 |
EJB31_RS25370 (EJB31_25440) | 85805..86221 | + | 417 | WP_014343494 | conjugal transfer relaxosome DNA-binding protein TraM | - |
EJB31_RS25375 (EJB31_25445) | 86421..87158 | + | 738 | WP_013214018 | conjugal transfer protein TrbJ | - |
EJB31_RS25380 (EJB31_25450) | 87273..87479 | + | 207 | WP_171773970 | TraY domain-containing protein | - |
EJB31_RS25385 (EJB31_25455) | 87541..87909 | + | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
EJB31_RS25390 (EJB31_25460) | 87923..88228 | + | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
EJB31_RS25395 (EJB31_25465) | 88248..88814 | + | 567 | WP_004144423 | type IV conjugative transfer system protein TraE | traE |
EJB31_RS25400 (EJB31_25470) | 88801..89541 | + | 741 | WP_013214019 | type-F conjugative transfer system secretin TraK | traK |
EJB31_RS25405 (EJB31_25475) | 89541..90965 | + | 1425 | WP_013214020 | F-type conjugal transfer pilus assembly protein TraB | traB |
EJB31_RS25410 (EJB31_25480) | 91037..91621 | + | 585 | WP_014343493 | type IV conjugative transfer system lipoprotein TraV | traV |
EJB31_RS25420 (EJB31_25485) | 91944..92162 | + | 219 | WP_004195468 | hypothetical protein | - |
EJB31_RS25425 (EJB31_25490) | 92163..92474 | + | 312 | WP_109217548 | hypothetical protein | - |
EJB31_RS25430 (EJB31_25495) | 92541..92945 | + | 405 | WP_004197817 | hypothetical protein | - |
EJB31_RS25440 (EJB31_25510) | 93322..93720 | + | 399 | WP_014343490 | hypothetical protein | - |
EJB31_RS25445 (EJB31_25515) | 93792..96431 | + | 2640 | WP_013214024 | type IV secretion system protein TraC | virb4 |
EJB31_RS25450 (EJB31_25520) | 96431..96820 | + | 390 | WP_013214025 | type-F conjugative transfer system protein TrbI | - |
EJB31_RS25455 (EJB31_25525) | 96820..97455 | + | 636 | WP_013214026 | type-F conjugative transfer system protein TraW | traW |
EJB31_RS25460 (EJB31_25530) | 97490..97891 | + | 402 | WP_004194979 | hypothetical protein | - |
EJB31_RS25465 (EJB31_25535) | 97888..98877 | + | 990 | WP_013214027 | conjugal transfer pilus assembly protein TraU | traU |
EJB31_RS25470 (EJB31_25540) | 98890..99528 | + | 639 | WP_004193871 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
EJB31_RS25475 (EJB31_25545) | 99587..101542 | + | 1956 | WP_014343489 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
EJB31_RS25480 (EJB31_25550) | 101574..101828 | + | 255 | WP_004152674 | conjugal transfer protein TrbE | - |
EJB31_RS25485 (EJB31_25555) | 101806..102054 | + | 249 | WP_013214029 | hypothetical protein | - |
EJB31_RS25490 (EJB31_25560) | 102067..102393 | + | 327 | WP_013214030 | hypothetical protein | - |
EJB31_RS25495 (EJB31_25565) | 102414..103166 | + | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
EJB31_RS25500 (EJB31_25570) | 103177..103416 | + | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
EJB31_RS25505 (EJB31_25575) | 103388..103945 | + | 558 | WP_013214031 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
EJB31_RS25510 (EJB31_25580) | 103991..104434 | + | 444 | WP_013214032 | F-type conjugal transfer protein TrbF | - |
EJB31_RS25515 (EJB31_25585) | 104412..105791 | + | 1380 | WP_074160414 | conjugal transfer pilus assembly protein TraH | traH |
EJB31_RS25520 (EJB31_25590) | 105791..108613 | + | 2823 | WP_013214034 | conjugal transfer mating-pair stabilization protein TraG | traG |
EJB31_RS25525 (EJB31_25595) | 108636..109238 | + | 603 | WP_013214035 | conjugal transfer protein TraS | - |
EJB31_RS25530 (EJB31_25600) | 109489..110220 | + | 732 | WP_013214036 | conjugal transfer complement resistance protein TraT | - |
EJB31_RS25535 | 110413..111102 | + | 690 | WP_014343485 | hypothetical protein | - |
EJB31_RS25540 (EJB31_25610) | 111229..113541 | + | 2313 | WP_013214038 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
ID | 1828 | GenBank | NZ_RSEZ01000005 |
Plasmid name | pK012_2 | Incompatibility group | IncR |
Plasmid size | 146262 bp | Coordinate of oriT [Strand] | 85487..85536 [-] |
Host baterium | Klebsiella pneumoniae strain 12 |
Cargo genes
Drug resistance gene | catA2, sul2, aac(3)-IId, blaTEM-1B, blaCTX-M-15, dfrA30 |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | AcrIE9 |