Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101320
Name   oriT_EPA165|unnamed in_silico
Organism   Escherichia coli strain EPA165
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_SJTD01000026 (5542..5630 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_EPA165|unnamed
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1245 GenBank   WP_131505435
Name   nikB_E0D72_RS23625_EPA165|unnamed insolico UniProt ID   _
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103801.28 Da        Isoelectric Point: 7.3322

>WP_131505435.1 IncI1-type relaxase NikB [Escherichia coli]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFCGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure



No available structure.




Auxiliary protein

ID   436 GenBank   WP_047669560
Name   WP_047669560_EPA165|unnamed insolico UniProt ID   _
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12599.58 Da        Isoelectric Point: 10.6897

>WP_047669560.1 IncI1-type relaxosome accessory protein NikA [Escherichia coli]
MSDSAVKKKSEVRQKTVVRTLRFSPIEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure



No available structure.




T4CP

ID   854 GenBank   WP_001289276
Name   trbC_E0D72_RS23630_EPA165|unnamed insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 11008..49305

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E0D72_RS23630 (E0D72_23630) 8724..11015 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
E0D72_RS23635 (E0D72_23635) 11008..12078 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
E0D72_RS23640 (E0D72_23640) 12097..13305 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
E0D72_RS23645 (E0D72_23645) 13597..13749 + 153 WP_001331364 Hok/Gef family protein -
E0D72_RS23650 (E0D72_23650) 13821..14072 - 252 WP_001291964 hypothetical protein -
E0D72_RS26140 14571..14666 + 96 WP_001303310 DinQ-like type I toxin DqlB -
E0D72_RS25475 14731..14907 - 177 WP_001054897 hypothetical protein -
E0D72_RS23655 (E0D72_23655) 15299..15508 + 210 WP_000062603 HEAT repeat domain-containing protein -
E0D72_RS23660 (E0D72_23660) 15580..16242 - 663 WP_000653334 plasmid IncI1-type surface exclusion protein ExcA -
E0D72_RS23665 (E0D72_23665) 16307..18469 - 2163 WP_000698351 DotA/TraY family protein traY
E0D72_RS23670 (E0D72_23670) 18566..19150 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
E0D72_RS23675 (E0D72_23675) 19179..20381 - 1203 WP_024261752 IncI1-type conjugal transfer protein TraW traW
E0D72_RS23680 (E0D72_23680) 20348..20962 - 615 WP_000337398 IncI1-type conjugal transfer protein TraV traV
E0D72_RS23685 (E0D72_23685) 20962..24006 - 3045 WP_001024780 IncI1-type conjugal transfer protein TraU traU
E0D72_RS23690 (E0D72_23690) 24096..24896 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
E0D72_RS23695 (E0D72_23695) 24880..25068 - 189 WP_001277255 putative conjugal transfer protein TraS -
E0D72_RS23700 (E0D72_23700) 25132..25536 - 405 WP_000086957 IncI1-type conjugal transfer protein TraR traR
E0D72_RS23705 (E0D72_23705) 25587..26114 - 528 WP_001055569 conjugal transfer protein TraQ traQ
E0D72_RS23710 (E0D72_23710) 26114..26818 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
E0D72_RS23715 (E0D72_23715) 26818..28107 - 1290 WP_001272005 conjugal transfer protein TraO traO
E0D72_RS23720 (E0D72_23720) 28110..29093 - 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
E0D72_RS23725 (E0D72_23725) 29104..29796 - 693 WP_000138548 DotI/IcmL family type IV secretion protein traM
E0D72_RS23730 (E0D72_23730) 29793..30140 - 348 WP_001055900 conjugal transfer protein traL
E0D72_RS23735 (E0D72_23735) 30158..33925 - 3768 WP_001141529 LPD7 domain-containing protein -
E0D72_RS23740 (E0D72_23740) 34015..34566 - 552 WP_000014583 phospholipase D family protein -
E0D72_RS23745 (E0D72_23745) 34581..34871 - 291 WP_001299214 hypothetical protein traK
E0D72_RS23750 (E0D72_23750) 34868..36016 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
E0D72_RS23755 (E0D72_23755) 36013..36831 - 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
E0D72_RS23760 (E0D72_23760) 36828..37286 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
E0D72_RS23765 (E0D72_23765) 37681..38265 - 585 WP_000977522 histidine phosphatase family protein -
E0D72_RS23770 (E0D72_23770) 38325..39527 - 1203 WP_000976351 conjugal transfer protein TraF -
E0D72_RS23775 (E0D72_23775) 39612..40436 - 825 WP_001238939 conjugal transfer protein TraE traE
E0D72_RS23780 (E0D72_23780) 40587..41741 - 1155 WP_001139957 site-specific integrase -
E0D72_RS26020 41740..42093 + 354 WP_001393368 hypothetical protein -
E0D72_RS26225 (E0D72_23815) 43157..43378 + 222 Protein_44 prepilin -
E0D72_RS23820 (E0D72_23820) 43375..44799 - 1425 WP_001389385 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
E0D72_RS23825 (E0D72_23825) 44799..45455 - 657 WP_001193553 prepilin peptidase -
E0D72_RS23830 (E0D72_23830) 45440..46000 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
E0D72_RS23835 (E0D72_23835) 46010..46624 - 615 WP_000959785 type 4 pilus major pilin -
E0D72_RS23840 (E0D72_23840) 46642..47739 - 1098 WP_001208805 type II secretion system F family protein -
E0D72_RS23845 (E0D72_23845) 47752..49305 - 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
E0D72_RS23850 (E0D72_23850) 49316..49768 - 453 WP_001247336 type IV pilus biogenesis protein PilP -
E0D72_RS23855 (E0D72_23855) 49755..51050 - 1296 WP_000752774 type 4b pilus protein PilO2 -
E0D72_RS23860 (E0D72_23860) 51043..52725 - 1683 WP_032311054 PilN family type IVB pilus formation outer membrane protein -
E0D72_RS23865 (E0D72_23865) 52739..53176 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
E0D72_RS23870 (E0D72_23870) 53176..54243 - 1068 WP_000742600 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   1764 GenBank   NZ_SJTD01000026
Plasmid name   EPA165|unnamed Incompatibility group   -
Plasmid size   58169 bp Coordinate of oriT [Strand]   5542..5630 [-]
Host baterium   Escherichia coli strain EPA165

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -