Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 101313
• Name: oriT_p9
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC33-35
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_JAKNUR010000011 (6630..6661 [-], 32 nt)
• oriT length: 32 nt
• IRs (inverted repeats): 3..10, 14..21 (ACATTGTC..GACAATGT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 32 nt

>oriT_p9
GCACATTGTCGAAGACAATGTATAAGCGCGCA

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobQ_MCQ68_RS24885_p9

• ID: 1244
• GenBank: WP_224557662
• Name: mobQ_MCQ68_RS24885_p9
• UniProt ID: _
• Length: 427 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 427 a.a.
• Molecular weight: 48285.83 Da
• Isoelectric Point: 9.4406

>WP_224557662.1 MobQ family relaxase [Pseudosulfitobacter pseudonitzschiae]
MAIYHLSAKVISRAGGRSSVAAAAYRTAGRLRDDRQGLEHDYSRKGGVVHSEIMAPDNAPDWMRDRDQLW
NAVEAVEKRRDAQLAREIEVALPRELDRGARLELLRGFVQREFVDRGMIADVAIHEGKARDGQSQPHAHV
MLTMRELTGEGFGKKDRSWNAPDLLMGWRAAWARDANTALERAGRSERIDHRSLDVQRAEAEQQAERARG
GGQEELALEHEKKVIELSREAEPKIGPSANAQEKRGIVTERGEAFRAAQARNAQRQELGWRQLELRFELM
ERGRAFIATARERLDQLWGRAEAAMSRIRERVMGEVERPQTGAPRGDMDARRAAVLGRDVDRPEVQAPDV
GRERVVPDDPIRERLNAILGRGRDIGQERDAAAVERDIAARDSEGRDQGRRDDILGRGRDTASERGQGRD
RDRDRER

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-202)

Protein structure

No available structure.

T4CP

t4cp2_MCQ68_RS24645_p9

• ID: 853
• GenBank: WP_224557659
• Name: t4cp2_MCQ68_RS24645_p9
• UniProt ID: _
• Length: 614 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 614 a.a.
• Molecular weight: 67438.85 Da
• Isoelectric Point: 9.8245

>WP_224557659.1 type IV secretory system conjugative DNA transfer family protein [Pseudosulfitobacter pseudonitzschiae]
MSFVLEARHWVIMIGVVILAAAALVFAPQAVAIYPVTTYAFPIIAVAVILDTLGTAAERHRAPLKLLAWV
CLCAATLTALTPLRGPLSDILATVQAWTGAGWPLPRAIWEGLKGLARYSDPQKQAMAISFALGAFGVAVA
VSTPLMAVFNPRIGRNRKSRTGPWQAGWMDPRDVAQLKRNKTGLPLALHKGKLLRYVKNDAKGWRGGHHL
VVSGTRGGKGVSAVIPAILDHQGPVVVLDIKGENFAVTRRHREELGRKVAVLNPFGLVEDGKDQFNPLDY
IRPHELARDVALVADGLVKPEQGDGAHFSEMARQLVAAAIEVIVTQEEPNRRNLIAVADLLLSANLDGTL
EAWAENSDLVGHRPAQTAATILRAGDRERGSIQTAVSKAFEWMQSDNMRHFLAGSSFHMDDLLDDRVDLF
IAVPLDQVDKQAIFMRLFINLVLGTVVRQDGRRKVKAPILLVLDEFVRMGRMEQIMNIANVAAGAGVEAL
FVTQDTGQVEKAYGPNDARSIFGSCITKRVFNLNDIETAEWAARHLGESTVYSQQIREGKAPNEGRDFSY
SEQRQKLMTAEQITGMKADDLLLLVGNRNPLKAKQNVYFKSRAYRGRFDQNPLN

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (181-604)

Protein structure

No available structure.

Host bacterium

• ID: 1757
• GenBank: NZ_JAKNUR010000011
• Plasmid name: p9
• Incompatibility group: -
• Plasmid size: 37727 bp
• Coordinate of oriT [Strand]: 6630..6661 [-]
• Host baterium: Pseudosulfitobacter pseudonitzschiae strain SC33-35

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -