Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101294 |
| Name | oriT_EfG2-21|unnamed  |
| Organism | Verminephrobacter eiseniae strain EfG2-21 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_QZDD01000002 (12816..12914 [-], 99 nt) |
| oriT length | 99 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 99 nt
>oriT_EfG2-21|unnamed
GAATAAGGGGAGGCAAAGATAGATAGCCGGCTTGCCGATTAGCTAACTTAGCCAATCTTGCCCGCCTTACGGCGTTAATAACACCAAAGAAAGTCTACA
GAATAAGGGGAGGCAAAGATAGATAGCCGGCTTGCCGATTAGCTAACTTAGCCAATCTTGCCCGCCTTACGGCGTTAATAACACCAAAGAAAGTCTACA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1237 | GenBank | WP_265290282 |
| Name | traI_D5047_RS24025_EfG2-21|unnamed |
UniProt ID | _ |
| Length | 745 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 745 a.a. Molecular weight: 82111.97 Da Isoelectric Point: 10.7343
>WP_265290282.1 TraI/MobA(P) family conjugative relaxase [Verminephrobacter eiseniae]
MILKHVPMRSLGKSDFAGLANYITNAQSKEHRLGEVRLTNCHAGTVGDAITEVLATQHTNTRATGDKTYH
LLVSFPLGENLPASVLQQVEERVCAALGYAEHQRISAVHNDTDHLHIHIAINKIHPVRNTMHEPFQAYRT
MADVAVKLEKEFGLQLVNHSSKRTLAEGRAGDMEQHAGVESLVGWIRRECLDEIKGAQSWQDLHQVLRDN
SLELRVRVNGFVIEAGDGTMVKASTVARDLSKPSLDARLGPFEASPERQAQTTAKRQYRKDPIRLRVNTV
ELYAKYKAEQQSLTTARGQALERARHRKDRLIEAAKWSNRLRRATIKVIGEGRTNKKLLYTQASKALRSE
IQAINKQFQQERAALYAEHSRRTWADWLKKEAHHGNTEALAALRAREKTGLKGNTIQGEGQAKPGHAPAV
DNITKKGTIIFRAGMSAVRDDGDRLQVSRETTREGLQEALRLAMQRYGNRITVNGTVEFKAQIIRAAVDS
QLPITFADPALESRRQALLNKENTHERTERPEHRGRAGRGAGGPGQRPAADQHATGAAADARAGDGRPAA
DRGDRADAGLHAASVHRKPDVGRLGRKPPPQSQNRLRALSELGVVRIAGGSEVLLPRDVPRHVEQQGTQP
DHALRRGISRPGTGVGQTLPGVAAADKYITEREAKRLKCFDIPKHSRYTAGDDALTFQGIRNVEGQALAL
LKRGDEVMVMPIDQATARRLTRIAVGDAVSITAKGSIKTSKGRSR
MILKHVPMRSLGKSDFAGLANYITNAQSKEHRLGEVRLTNCHAGTVGDAITEVLATQHTNTRATGDKTYH
LLVSFPLGENLPASVLQQVEERVCAALGYAEHQRISAVHNDTDHLHIHIAINKIHPVRNTMHEPFQAYRT
MADVAVKLEKEFGLQLVNHSSKRTLAEGRAGDMEQHAGVESLVGWIRRECLDEIKGAQSWQDLHQVLRDN
SLELRVRVNGFVIEAGDGTMVKASTVARDLSKPSLDARLGPFEASPERQAQTTAKRQYRKDPIRLRVNTV
ELYAKYKAEQQSLTTARGQALERARHRKDRLIEAAKWSNRLRRATIKVIGEGRTNKKLLYTQASKALRSE
IQAINKQFQQERAALYAEHSRRTWADWLKKEAHHGNTEALAALRAREKTGLKGNTIQGEGQAKPGHAPAV
DNITKKGTIIFRAGMSAVRDDGDRLQVSRETTREGLQEALRLAMQRYGNRITVNGTVEFKAQIIRAAVDS
QLPITFADPALESRRQALLNKENTHERTERPEHRGRAGRGAGGPGQRPAADQHATGAAADARAGDGRPAA
DRGDRADAGLHAASVHRKPDVGRLGRKPPPQSQNRLRALSELGVVRIAGGSEVLLPRDVPRHVEQQGTQP
DHALRRGISRPGTGVGQTLPGVAAADKYITEREAKRLKCFDIPKHSRYTAGDDALTFQGIRNVEGQALAL
LKRGDEVMVMPIDQATARRLTRIAVGDAVSITAKGSIKTSKGRSR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 846 | GenBank | WP_265290283 |
| Name | t4cp2_D5047_RS24030_EfG2-21|unnamed |
UniProt ID | _ |
| Length | 637 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 637 a.a. Molecular weight: 70063.39 Da Isoelectric Point: 8.6762
>WP_265290283.1 type IV secretory system conjugative DNA transfer family protein [Verminephrobacter eiseniae]
MKIKMNNAVGPQVRTAKPKPSKLLPVLGTASMVGGLQAATQFFAHTFAYHATLGPNVGHVYAPWSILHWT
YKWYSQYLDEIMKAGSMGMLVSTVGLLGVAVAKVVTSNSSKANEYLHGSARWAEKKDIQAAGLLPRERNV
LEIVTGKAAPTATGVYVGGWQDKDGNFFYLRHSGPEHVLTYAPTRSGKGVGLVVPTLLSWGTSSVITDLK
GELWALTAGWRQKHAKNKVLRFEPASISGGVCWNPLDEIRLGTEYEVGDVQNLATLIVDPDGKGLDSHWQ
KTAFALLVGVILHALYKAKDEVGTATLPSIDAMLADPSRDIGELWMEMATYGHVDGQNHHAISSAARDMM
DRPEEEAGSVLSTAKSYLALYRDPVVARNVSRSDFRIKQLMHEDDPVSLYIVTQPNDKARLRPLVRVMVN
MIVRLLADKMDFEGGRPVAHYKHRLLMMLDEFPSLGKLEIMQESLAFVAGYGIKCYLICQDINQLKSRET
GYGHDESITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRTAAMLGQVSRTYQEVQRPLLT
PDECLRMPGPKKNAQGEIKEAGDMVIYVAGYPSIYGKQPLYFKDSVFSARAAIPAPKVSDRLRAVAQAET
EGEGITI
MKIKMNNAVGPQVRTAKPKPSKLLPVLGTASMVGGLQAATQFFAHTFAYHATLGPNVGHVYAPWSILHWT
YKWYSQYLDEIMKAGSMGMLVSTVGLLGVAVAKVVTSNSSKANEYLHGSARWAEKKDIQAAGLLPRERNV
LEIVTGKAAPTATGVYVGGWQDKDGNFFYLRHSGPEHVLTYAPTRSGKGVGLVVPTLLSWGTSSVITDLK
GELWALTAGWRQKHAKNKVLRFEPASISGGVCWNPLDEIRLGTEYEVGDVQNLATLIVDPDGKGLDSHWQ
KTAFALLVGVILHALYKAKDEVGTATLPSIDAMLADPSRDIGELWMEMATYGHVDGQNHHAISSAARDMM
DRPEEEAGSVLSTAKSYLALYRDPVVARNVSRSDFRIKQLMHEDDPVSLYIVTQPNDKARLRPLVRVMVN
MIVRLLADKMDFEGGRPVAHYKHRLLMMLDEFPSLGKLEIMQESLAFVAGYGIKCYLICQDINQLKSRET
GYGHDESITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRTAAMLGQVSRTYQEVQRPLLT
PDECLRMPGPKKNAQGEIKEAGDMVIYVAGYPSIYGKQPLYFKDSVFSARAAIPAPKVSDRLRAVAQAET
EGEGITI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 847 | GenBank | WP_265290260 |
| Name | t4cp2_D5047_RS24150_EfG2-21|unnamed |
UniProt ID | _ |
| Length | 852 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 852 a.a. Molecular weight: 94207.38 Da Isoelectric Point: 6.3055
>WP_265290260.1 VirB4 family type IV secretion/conjugal transfer ATPase [Verminephrobacter eiseniae]
MIEAIAIAIAVLGAVLLLILFARIRAVDAELKLQKHRAKDAGLADLLNYAAVVDDGVIVGKNGSFMAAWL
YKGDDNASSTEEQREIVSFRINQALAVLGNGWAVHVDAVRRPAPNYSERGVSSFPDSVSSAIDEERRRLF
EGLGAMFEGYFVLAVTWFPPVLAQRKFVELMFDDDAVAPDHTARTTGLISQFKREITSLESRLSSAVKMT
RLRSQKVVLEEGREVTHDDFLSWLQFCVTGLHHPLMLPSNPMYLDALIGGQELWGGVVPKIGRKFIQVVA
IEGFPLESTPGVLSALAELPCEYRWSSRFIFMDQHESLKHLDKFRKKWKQKIRGFFDQVFNTNTGSINQD
AAAMVGDADAAIAEVNSGLVAAGYYTSVVVLMDEDRERLAASALQVEKAINRLAFAARIETINTLDAYLG
SLPGHGVENVRRPLINTMNLADLLPTSSIWTGSATAPCPMYPPLSPALMHCVTVGATPFRLNLHVRDLGH
TFMFGPTGAGKSTHLGIIAAQLRRYAGMSIYAFDKGMSMYPLAAGIRAATKGKSGLHFTVAADDDRLAFC
PLQFLETKGDRAWAMEWIDTILTLNGVNTTPAQRNEIGNAIMSMHGSGARTLSEFSVTIQDEAIREAIRQ
YTVDGLMGHLLDAEEDGLSLSDFTVFEIEELMNLGEKFALPVLLYLFRRIERALKGQPAVIILDEAWLML
GHPAFRAKIREWLKVLRKANCLVLMATQSLSDAANSGILDVIVESTATKIFLPNVYARDEDTSALYRRMG
LNARQIEILATAIPKRQYYYVSENGRRLYDLALGPMALAFVGASDKESVATIKRLEANFDHEWMREWLAG
RGLNLNDHLEAT
MIEAIAIAIAVLGAVLLLILFARIRAVDAELKLQKHRAKDAGLADLLNYAAVVDDGVIVGKNGSFMAAWL
YKGDDNASSTEEQREIVSFRINQALAVLGNGWAVHVDAVRRPAPNYSERGVSSFPDSVSSAIDEERRRLF
EGLGAMFEGYFVLAVTWFPPVLAQRKFVELMFDDDAVAPDHTARTTGLISQFKREITSLESRLSSAVKMT
RLRSQKVVLEEGREVTHDDFLSWLQFCVTGLHHPLMLPSNPMYLDALIGGQELWGGVVPKIGRKFIQVVA
IEGFPLESTPGVLSALAELPCEYRWSSRFIFMDQHESLKHLDKFRKKWKQKIRGFFDQVFNTNTGSINQD
AAAMVGDADAAIAEVNSGLVAAGYYTSVVVLMDEDRERLAASALQVEKAINRLAFAARIETINTLDAYLG
SLPGHGVENVRRPLINTMNLADLLPTSSIWTGSATAPCPMYPPLSPALMHCVTVGATPFRLNLHVRDLGH
TFMFGPTGAGKSTHLGIIAAQLRRYAGMSIYAFDKGMSMYPLAAGIRAATKGKSGLHFTVAADDDRLAFC
PLQFLETKGDRAWAMEWIDTILTLNGVNTTPAQRNEIGNAIMSMHGSGARTLSEFSVTIQDEAIREAIRQ
YTVDGLMGHLLDAEEDGLSLSDFTVFEIEELMNLGEKFALPVLLYLFRRIERALKGQPAVIILDEAWLML
GHPAFRAKIREWLKVLRKANCLVLMATQSLSDAANSGILDVIVESTATKIFLPNVYARDEDTSALYRRMG
LNARQIEILATAIPKRQYYYVSENGRRLYDLALGPMALAFVGASDKESVATIKRLEANFDHEWMREWLAG
RGLNLNDHLEAT
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 31340..41016
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| D5047_RS24070 (D5047_23905) | 26622..26808 | + | 187 | Protein_29 | Tn3 family transposase | - |
| D5047_RS24075 | 26846..26974 | - | 129 | WP_265290289 | hypothetical protein | - |
| D5047_RS24080 (D5047_23910) | 27026..27901 | + | 876 | WP_265290247 | recombinase family protein | - |
| D5047_RS24085 (D5047_23915) | 27903..28577 | - | 675 | WP_265290248 | DNA adenine methylase | - |
| D5047_RS24090 (D5047_23920) | 28733..29164 | - | 432 | WP_265290249 | OmpA family protein | - |
| D5047_RS24095 (D5047_23925) | 29180..29815 | - | 636 | WP_265290288 | TraX family protein | - |
| D5047_RS24100 (D5047_23930) | 29892..30113 | - | 222 | WP_265290250 | hypothetical protein | - |
| D5047_RS24105 (D5047_23935) | 30110..30724 | - | 615 | WP_265290251 | transglycosylase SLT domain-containing protein | - |
| D5047_RS24110 (D5047_23940) | 30738..31322 | - | 585 | WP_265290252 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| D5047_RS24115 (D5047_23945) | 31340..33100 | - | 1761 | WP_265290253 | P-type conjugative transfer protein TrbL | virB6 |
| D5047_RS24120 (D5047_23950) | 33134..33358 | - | 225 | WP_265290254 | entry exclusion lipoprotein TrbK | - |
| D5047_RS24125 (D5047_23955) | 33368..34132 | - | 765 | WP_265290255 | P-type conjugative transfer protein TrbJ | virB5 |
| D5047_RS24130 (D5047_23960) | 34153..35538 | - | 1386 | WP_265290256 | TrbI/VirB10 family protein | virB10 |
| D5047_RS24135 (D5047_23965) | 35543..35986 | - | 444 | WP_265290257 | conjugal transfer protein TrbH | - |
| D5047_RS24140 (D5047_23970) | 35989..36888 | - | 900 | WP_265290258 | P-type conjugative transfer protein TrbG | virB9 |
| D5047_RS24145 (D5047_23975) | 36906..37685 | - | 780 | WP_265290259 | conjugal transfer protein TrbF | virB8 |
| D5047_RS24150 (D5047_23980) | 37682..40240 | - | 2559 | WP_265290260 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| D5047_RS24155 (D5047_23985) | 40237..40548 | - | 312 | WP_265290261 | conjugal transfer protein TrbD | virB3 |
| D5047_RS24160 (D5047_23990) | 40552..41016 | - | 465 | WP_265290262 | conjugal transfer system pilin TrbC | virB2 |
Host bacterium
| ID | 1738 | GenBank | NZ_QZDD01000002 |
| Plasmid name | EfG2-21|unnamed | Incompatibility group | IncP1 |
| Plasmid size | 41826 bp | Coordinate of oriT [Strand] | 12816..12914 [-] |
| Host baterium | Verminephrobacter eiseniae strain EfG2-21 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |