Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101277
Name   oriT_FEX725|unnamed6 in_silico
Organism   Escherichia coli strain FEX725
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_PSOV01000026 (29624..29972 [+], 349 nt)
oriT length   349 nt
IRs (inverted repeats)      134..140, 154..160  (AAAATAT..ATATTTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 349 nt

>oriT_FEX725|unnamed6
ACTGCGATGCAGGATAGGGCAAACGCCGCAAAATGACGTCTCTGACGCCATTCCGAAACGTTTGCCCGATGACCTACAGTCGGTTCTTGTTGGAGAGTTTTATGAAATATTTGGTCAAAGAGTTTATTAACGAAAAATATACTAAGGCTGTTAATATTTTAAAGGATAACCTTAAAGAACACTATCATGTTTTTTATTGTGTGAGATTAAGTGAGATTCTTTTTCCTGCCAGTGAATATGGAACTGAACAGTTTTTTAGTGATTTTGAAAAGATAAACTCCATTTCATTACCTGTCTTAGTGTTTGATCTCAAAGAGAGTGTTCCTGTGATTGTCATTAGTCTTGATGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1232 GenBank   WP_000539529
Name   mobP1_C4M78_RS25655_FEX725|unnamed6 insolico UniProt ID   A0A0F7JE92
Length   388 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 388 a.a.        Molecular weight: 44493.85 Da        Isoelectric Point: 10.5286

>WP_000539529.1 MULTISPECIES: MobP1 family relaxase [Enterobacteriaceae]
MGVYVDKEHRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAITRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFWGL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(70-228)


  Protein structure


Source ID Structure
AlphaFold DB A0A0F7JE92


T4CP

ID   843 GenBank   WP_000053818
Name   t4cp2_C4M78_RS25590_FEX725|unnamed6 insolico UniProt ID   _
Length   611 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 611 a.a.        Molecular weight: 69331.19 Da        Isoelectric Point: 7.4339

>WP_000053818.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGANPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(102-571)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 11462..21478

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4M78_RS25575 (C4M78_25580) 7877..8800 + 924 WP_000902514 SMEK domain-containing protein -
C4M78_RS25580 (C4M78_25585) 8915..9217 - 303 WP_000717624 TrbM/KikA/MpfK family conjugal transfer protein -
C4M78_RS25585 (C4M78_25590) 9214..9627 - 414 WP_000722603 cag pathogenicity island Cag12 family protein -
C4M78_RS25590 (C4M78_25595) 9624..11459 - 1836 WP_000053818 type IV secretory system conjugative DNA transfer family protein -
C4M78_RS25595 (C4M78_25600) 11462..12493 - 1032 WP_001058465 P-type DNA transfer ATPase VirB11 virB11
C4M78_RS25600 (C4M78_25605) 12495..13700 - 1206 WP_000139139 VirB10/TraB/TrbI family type IV secretion system protein virB10
C4M78_RS25605 (C4M78_25610) 13697..14626 - 930 WP_000783379 TrbG/VirB9 family P-type conjugative transfer protein virB9
C4M78_RS25610 (C4M78_25615) 14631..15344 - 714 WP_000394613 type IV secretion system protein virB8
C4M78_RS25615 (C4M78_25620) 15589..16719 - 1131 WP_000524912 type IV secretion system protein virB6
C4M78_RS25620 (C4M78_25625) 16728..17015 - 288 WP_000835347 EexN family lipoprotein -
C4M78_RS25625 (C4M78_25630) 17016..17774 - 759 WP_104274903 type IV secretion system protein -
C4M78_RS25630 (C4M78_25635) 17785..20538 - 2754 WP_016731577 VirB3 family type IV secretion system protein virb4
C4M78_RS25635 (C4M78_25640) 20557..20853 - 297 WP_000916182 TrbC/VirB2 family protein virB2
C4M78_RS25640 (C4M78_25645) 20831..21478 - 648 WP_000953525 lytic transglycosylase domain-containing protein virB1
C4M78_RS25645 (C4M78_25650) 21511..21723 - 213 WP_000125172 hypothetical protein -
C4M78_RS25650 (C4M78_25655) 21653..22171 - 519 WP_001446885 transcription termination/antitermination NusG family protein -
C4M78_RS25655 (C4M78_25660) 22524..23690 - 1167 WP_000539529 MobP1 family relaxase -
C4M78_RS25660 (C4M78_25665) 23693..24238 - 546 WP_001436753 DNA distortion polypeptide 1 -
C4M78_RS25670 (C4M78_25675) 24564..24836 - 273 WP_000160399 hypothetical protein -
C4M78_RS28750 (C4M78_25680) 24849..24998 - 150 WP_000003880 hypothetical protein -
C4M78_RS25680 (C4M78_25685) 25068..25247 - 180 WP_000439078 hypothetical protein -
C4M78_RS25685 (C4M78_25690) 25290..25619 - 330 WP_000866649 hypothetical protein -
C4M78_RS25690 (C4M78_25695) 25712..25927 - 216 WP_001180118 hypothetical protein -
C4M78_RS25695 (C4M78_25700) 25917..26162 - 246 WP_000356542 hypothetical protein -

Host bacterium

ID   1721 GenBank   NZ_PSOV01000026
Plasmid name   FEX725|unnamed6 Incompatibility group   IncX3
Plasmid size   33661 bp Coordinate of oriT [Strand]   29624..29972 [+]
Host baterium   Escherichia coli strain FEX725

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -