Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101251 |
| Name | oriT_pB17  |
| Organism | Klebsiella pneumoniae strain B17 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_NTHV01000037 (26501..26605 [-], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 32..37, 40..45 (GCCGGC..GCCGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 105 nt
>oriT_pB17
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1219 | GenBank | WP_004206924 |
| Name | traI_CK502_RS25960_pB17 |
UniProt ID | A0A2V4FJ40 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75087.84 Da Isoelectric Point: 10.0285
>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2V4FJ40 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 4732..23703
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CK502_RS25845 (CK502_25835) | 148..1008 | - | 861 | Protein_0 | IS4-like element IS186B family transposase | - |
| CK502_RS28445 | 1098..1193 | - | 96 | WP_004206884 | DinQ-like type I toxin DqlB | - |
| CK502_RS25850 (CK502_25840) | 2420..3475 | - | 1056 | WP_021526650 | plasmid replication initiator RepA | - |
| CK502_RS25855 (CK502_25845) | 3772..4002 | - | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
| CK502_RS25860 (CK502_25850) | 4076..4729 | - | 654 | WP_004206935 | hypothetical protein | - |
| CK502_RS25865 (CK502_25855) | 4732..6912 | - | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| CK502_RS25870 (CK502_25860) | 6905..7555 | - | 651 | WP_004187488 | hypothetical protein | - |
| CK502_RS25875 (CK502_25865) | 7552..8760 | - | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| CK502_RS25880 (CK502_25870) | 8757..11807 | - | 3051 | WP_011154474 | conjugative transfer protein | traU |
| CK502_RS25885 (CK502_25875) | 11804..12298 | - | 495 | WP_004187480 | hypothetical protein | - |
| CK502_RS25890 (CK502_25880) | 12344..12733 | - | 390 | WP_011154472 | DUF6750 family protein | traR |
| CK502_RS25895 (CK502_25885) | 12750..13280 | - | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| CK502_RS25900 (CK502_25890) | 13304..14008 | - | 705 | WP_045342064 | conjugal transfer protein TraP | traP |
| CK502_RS25905 (CK502_25895) | 14020..15369 | - | 1350 | WP_004206932 | conjugal transfer protein TraO | traO |
| CK502_RS25910 (CK502_25900) | 15381..16532 | - | 1152 | WP_015062843 | DotH/IcmK family type IV secretion protein | traN |
| CK502_RS25915 (CK502_25905) | 16541..17254 | - | 714 | WP_223294958 | DotI/IcmL family type IV secretion protein | traM |
| CK502_RS25920 (CK502_25910) | 17322..17732 | + | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| CK502_RS25925 (CK502_25915) | 17719..17916 | + | 198 | WP_004187464 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| CK502_RS25930 (CK502_25920) | 17917..18429 | - | 513 | WP_011091071 | hypothetical protein | traL |
| CK502_RS28295 | 18395..21475 | - | 3081 | WP_227522518 | LPD7 domain-containing protein | - |
| CK502_RS25940 (CK502_25930) | 21500..21760 | - | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| CK502_RS25945 (CK502_25935) | 21750..22913 | - | 1164 | WP_045342066 | plasmid transfer ATPase TraJ | virB11 |
| CK502_RS25950 (CK502_25940) | 22924..23703 | - | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| CK502_RS25955 (CK502_25945) | 23700..24200 | - | 501 | WP_004206925 | DotD/TraH family lipoprotein | - |
| CK502_RS25960 (CK502_25950) | 24214..26193 | - | 1980 | WP_004206924 | TraI/MobA(P) family conjugative relaxase | - |
| CK502_RS25965 (CK502_25955) | 26180..26497 | - | 318 | WP_004206923 | plasmid mobilization protein MobA | - |
| CK502_RS25970 (CK502_25960) | 26773..27138 | + | 366 | WP_095885182 | MobC | - |
| CK502_RS25975 (CK502_25965) | 27639..28175 | - | 537 | WP_004206920 | hypothetical protein | - |
| CK502_RS25980 (CK502_25970) | 28308..28619 | - | 312 | WP_004187333 | hypothetical protein | - |
Host bacterium
| ID | 1695 | GenBank | NZ_NTHV01000037 |
| Plasmid name | pB17 | Incompatibility group | IncL/M |
| Plasmid size | 33451 bp | Coordinate of oriT [Strand] | 26501..26605 [-] |
| Host baterium | Klebsiella pneumoniae strain B17 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |