Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101246
Name   oriT_pKPC05 P51b in_silico
Organism   Klebsiella pneumoniae strain KPC05
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_NTGJ01000104 (32292..32390 [-], 99 nt)
oriT length   99 nt
IRs (inverted repeats)      26..31, 34..39  (GCCGGC..GCCGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 99 nt

>oriT_pKPC05 P51b
GGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1216 GenBank   WP_004206924
Name   traI_CK487_RS27935_pKPC05 P51b insolico UniProt ID   A0A2V4FJ40
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75087.84 Da        Isoelectric Point: 10.0285

>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A2V4FJ40


T4CP

ID   827 GenBank   WP_004206885
Name   t4cp2_CK487_RS27820_pKPC05 P51b insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79538.91 Da        Isoelectric Point: 4.8317

>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2380..29494

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CK487_RS27790 (CK487_27785) 36..401 - 366 WP_020277900 type II toxin-antitoxin system toxin endoribonuclease PemK-mt -
CK487_RS29390 370..627 - 258 WP_004187429 type II toxin-antitoxin system antitoxin PemI -
CK487_RS27795 (CK487_27790) 719..1372 - 654 WP_004206890 type II CAAX endopeptidase family protein -
CK487_RS27800 (CK487_27795) 1452..1835 + 384 WP_004206889 DUF1496 domain-containing protein -
CK487_RS27805 (CK487_27800) 1940..2335 + 396 WP_004187436 lytic transglycosylase domain-containing protein -
CK487_RS27810 (CK487_27805) 2380..3642 + 1263 WP_004206887 hypothetical protein trbA
CK487_RS27815 (CK487_27810) 3653..4603 + 951 WP_004206886 DsbC family protein trbB
CK487_RS27820 (CK487_27815) 4616..6703 + 2088 WP_004206885 conjugal transfer protein TrbC -
CK487_RS30605 6754..6849 - 96 WP_004206884 DinQ-like type I toxin DqlB -
CK487_RS27825 (CK487_27820) 8076..9143 - 1068 WP_024190316 plasmid replication initiator RepA -
CK487_RS27830 (CK487_27825) 9428..9658 - 231 WP_004187496 IncL/M type plasmid replication protein RepC -
CK487_RS27835 (CK487_27830) 9732..10385 - 654 WP_004206935 hypothetical protein -
CK487_RS27840 (CK487_27835) 10388..12568 - 2181 WP_004187492 DotA/TraY family protein traY
CK487_RS27845 (CK487_27840) 12561..13211 - 651 WP_004187488 hypothetical protein -
CK487_RS27850 (CK487_27845) 13208..14416 - 1209 WP_004187486 conjugal transfer protein TraW traW
CK487_RS27855 (CK487_27850) 14413..17463 - 3051 WP_011154474 conjugative transfer protein traU
CK487_RS27860 (CK487_27855) 17460..17954 - 495 WP_004187480 hypothetical protein -
CK487_RS27865 (CK487_27860) 18000..18389 - 390 WP_011154472 DUF6750 family protein traR
CK487_RS27870 (CK487_27865) 18406..18936 - 531 WP_004187478 conjugal transfer protein TraQ traQ
CK487_RS27875 (CK487_27870) 18960..19664 - 705 WP_004206933 conjugal transfer protein TraP traP
CK487_RS27880 (CK487_27875) 19676..21025 - 1350 WP_004206932 conjugal transfer protein TraO traO
CK487_RS27885 (CK487_27880) 21037..22188 - 1152 WP_015062843 DotH/IcmK family type IV secretion protein traN
CK487_RS27890 (CK487_27885) 22197..22910 - 714 WP_223294958 DotI/IcmL family type IV secretion protein traM
CK487_RS27895 (CK487_27890) 22978..23388 + 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
CK487_RS27900 (CK487_27895) 23375..23572 + 198 WP_004187464 Hha/YmoA family nucleoid-associated regulatory protein -
CK487_RS27905 (CK487_27900) 23573..24085 - 513 WP_011091071 hypothetical protein traL
CK487_RS30455 24051..27266 - 3216 WP_221178076 LPD7 domain-containing protein -
CK487_RS27915 (CK487_27910) 27291..27551 - 261 WP_004187310 IcmT/TraK family protein traK
CK487_RS27920 (CK487_27915) 27541..28704 - 1164 WP_004206926 plasmid transfer ATPase TraJ virB11
CK487_RS27925 (CK487_27920) 28715..29494 - 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
CK487_RS27930 (CK487_27925) 29491..29991 - 501 WP_004206925 DotD/TraH family lipoprotein -
CK487_RS27935 (CK487_27930) 30005..31984 - 1980 WP_004206924 TraI/MobA(P) family conjugative relaxase -
CK487_RS27940 (CK487_27935) 31971..32288 - 318 WP_004206923 plasmid mobilization protein MobA -
CK487_RS27945 (CK487_27940) 32567..32932 + 366 WP_004206922 hypothetical protein -
CK487_RS27950 (CK487_27945) 33433..33969 - 537 WP_004206920 hypothetical protein -
CK487_RS27955 (CK487_27950) 34102..34413 - 312 WP_004187333 hypothetical protein -

Host bacterium

ID   1690 GenBank   NZ_NTGJ01000104
Plasmid name   pKPC05 P51b Incompatibility group   IncL/M
Plasmid size   53079 bp Coordinate of oriT [Strand]   32292..32390 [-]
Host baterium   Klebsiella pneumoniae strain KPC05

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -