Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101243 |
| Name | oriT_FWSEC0066|unnamed6  |
| Organism | Escherichia coli strain FWSEC0066 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_RREL01000133 (10876..10928 [+], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_FWSEC0066|unnamed6
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 826 | GenBank | WP_000338972 |
| Name | t4cp2_C9Z16_RS26000_FWSEC0066|unnamed6 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73329.86 Da Isoelectric Point: 9.1391
>WP_000338972.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDISLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDISLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 29408..52413
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C9Z16_RS25860 (C9Z16_25915) | 24900..25352 | + | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
| C9Z16_RS25865 (C9Z16_25920) | 25345..25539 | + | 195 | WP_001127358 | DUF1187 family protein | - |
| C9Z16_RS25870 (C9Z16_25925) | 25585..26538 | + | 954 | WP_072097371 | SPFH domain-containing protein | - |
| C9Z16_RS25875 (C9Z16_25930) | 26600..27043 | + | 444 | WP_000498521 | NfeD family protein | - |
| C9Z16_RS26400 | 27047..27217 | + | 171 | WP_000550721 | hypothetical protein | - |
| C9Z16_RS25880 (C9Z16_25935) | 27228..27890 | + | 663 | WP_001243159 | hypothetical protein | - |
| C9Z16_RS25890 (C9Z16_25945) | 28041..28634 | + | 594 | WP_001243171 | hypothetical protein | - |
| C9Z16_RS25900 (C9Z16_25955) | 28848..29150 | + | 303 | WP_000189499 | hypothetical protein | - |
| C9Z16_RS25905 (C9Z16_25960) | 29147..29404 | + | 258 | WP_000739144 | hypothetical protein | - |
| C9Z16_RS25910 (C9Z16_25965) | 29408..30403 | + | 996 | WP_000276068 | type IV secretion system protein | virB6 |
| C9Z16_RS25915 (C9Z16_25970) | 30409..31050 | + | 642 | WP_001415805 | type IV secretion system protein | - |
| C9Z16_RS25920 (C9Z16_25975) | 31053..31307 | + | 255 | WP_000609210 | EexN family lipoprotein | - |
| C9Z16_RS25925 (C9Z16_25980) | 31360..32169 | - | 810 | WP_001005154 | DUF5710 domain-containing protein | - |
| C9Z16_RS25930 (C9Z16_25985) | 32217..32852 | + | 636 | WP_000835769 | hypothetical protein | - |
| C9Z16_RS25935 (C9Z16_25990) | 33024..33311 | + | 288 | WP_001326593 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| C9Z16_RS25940 (C9Z16_25995) | 33314..34549 | + | 1236 | WP_000733393 | toxin co-regulated pilus biosynthesis Q family protein | - |
| C9Z16_RS25945 (C9Z16_26000) | 34555..34992 | + | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| C9Z16_RS25950 (C9Z16_26005) | 35111..35509 | + | 399 | WP_001153669 | hypothetical protein | - |
| C9Z16_RS25955 (C9Z16_26010) | 35530..36114 | + | 585 | WP_001177114 | lytic transglycosylase domain-containing protein | virB1 |
| C9Z16_RS26490 | 36114..36404 | + | 291 | WP_000865478 | TrbC/VirB2 family protein | virB2 |
| C9Z16_RS25965 (C9Z16_26020) | 36475..36795 | + | 321 | WP_000362083 | VirB3 family type IV secretion system protein | virB3 |
| C9Z16_RS25970 (C9Z16_26025) | 36801..39158 | + | 2358 | WP_000548951 | VirB4 family type IV secretion system protein | virb4 |
| C9Z16_RS25980 (C9Z16_26035) | 39324..40058 | + | 735 | WP_000432283 | type IV secretion system protein | virB8 |
| C9Z16_RS25985 (C9Z16_26040) | 40124..40825 | + | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| C9Z16_RS25990 (C9Z16_26045) | 40815..41954 | + | 1140 | WP_000790639 | TrbI/VirB10 family protein | virB10 |
| C9Z16_RS25995 (C9Z16_26050) | 42037..43092 | + | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| C9Z16_RS26000 (C9Z16_26055) | 43108..45066 | + | 1959 | WP_000338972 | type IV secretory system conjugative DNA transfer family protein | - |
| C9Z16_RS26005 (C9Z16_26060) | 45117..46760 | + | 1644 | WP_001035589 | PilN family type IVB pilus formation outer membrane protein | - |
| C9Z16_RS26010 (C9Z16_26065) | 46811..48121 | + | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| C9Z16_RS26015 (C9Z16_26070) | 48105..48599 | + | 495 | WP_000912554 | type IV pilus biogenesis protein PilP | - |
| C9Z16_RS26020 (C9Z16_26075) | 48624..50162 | + | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| C9Z16_RS26025 (C9Z16_26080) | 50153..51262 | + | 1110 | WP_000974903 | type II secretion system F family protein | - |
| C9Z16_RS26030 (C9Z16_26085) | 51307..51864 | + | 558 | WP_000095048 | type 4 pilus major pilin | - |
| C9Z16_RS26035 (C9Z16_26090) | 51931..52413 | + | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| C9Z16_RS26040 (C9Z16_26095) | 52417..53052 | + | 636 | WP_000934978 | A24 family peptidase | - |
| C9Z16_RS26045 (C9Z16_26100) | 53065..54441 | + | 1377 | WP_000750519 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| C9Z16_RS27305 (C9Z16_26105) | 54438..54659 | - | 222 | Protein_68 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| C9Z16_RS26065 (C9Z16_26120) | 55299..56423 | + | 1125 | WP_000486720 | site-specific integrase | - |
| C9Z16_RS26070 (C9Z16_26125) | 56435..57088 | + | 654 | WP_000572549 | hypothetical protein | - |
Host bacterium
| ID | 1687 | GenBank | NZ_RREL01000133 |
| Plasmid name | FWSEC0066|unnamed6 | Incompatibility group | IncI2 |
| Plasmid size | 58087 bp | Coordinate of oriT [Strand] | 10876..10928 [+] |
| Host baterium | Escherichia coli strain FWSEC0066 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |