Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101238 |
| Name | oriT_pKPt3  |
| Organism | Klebsiella pneumoniae subsp. pneumoniae DSM 30104 = JCM 1662 = NBRC 14940 strain DSM 30104 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_AJJI01000025 (78507..80211 [+], 1705 nt) |
| oriT length | 1705 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 1705 nt
>oriT_pKPt3
GCCGTATGAATGACATCTATTTCCGTCCAATTCATCGAAACCAGTATTTTGAGGAAATGAAAGCATGATAAAGAAACTTAAAGTCATCATGTTGGCAGCAATAGTTACCGGATGTTCATCGCCGCCGCCTCCCGTTCCTGTGGAGTGGGATAAAGCCGCTGACCCATTAAATACCAGTTTACCGCAATGGAGTGACAATAACGTTATAGTCCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAGTTTCTACGATACAATATGGACTCCAGCAGTATTTTACGCAGTCGCCCATTCAACACGAATTGTCGTCACTGCACAATCCGGCACTGATTTCTTTAATGCCAAAAACTGGCTTCGCCAGAATGGCGCAAAAGGCGTTATTGAATACCAGCCTGTATTTAATTGCCTGACGTGCAGAGAAACCACTATTTATTTCTCTCACCAATCCATTTAAGCAACACAATCTAATAAGGATTTATCATGAAAAAAACCGTTATCGCGGCGACTGTATTATGCCTGACCGCAGGAACGCCGATTTATTCCTTTGCCGCTGACCCGTGCGAAGTTGTTCTTTGCATGTACGGAAAAGCCACAGGTAATAGTGGTGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATCAACGCATTTAAAAAACACCATCGTTTCAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGGGAGTGCAAAGCCGCAGACCCTGAATCAATAAGTAAAATCCTTTCAAAATTTGGCAGAGTGAAAGGATAACTGACATATCCATTTTGATATGAGAGGTTCGAAATGATAAACGAAGATTTATTCATTTAGAATATACACTCAAAAAATCAGGACAGAATATCTGTAGCCATGGGATATGACACGTTATCAAAAGAAGCCCACAGAGGTTGTGGGCTGTATTACGAAATATACGAAAGCTGTTTTATCGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAAGCTGACGCAAATAGATTAAGACGCTATGCCGAGAGTAAAGGTACGAAAATTGATGATGCGTCCTACAGTGAAGCACTGGAAGCTGAAAGGGAGTGCAGGGCTGAAATATACCGCGAGCAAATGTAATCACACAAAACAAAAGTGTATTGTACAATACAGAGACTAACAGGCGACGGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTTCGAAGAAATTGTTAAGACGAAAAATAATGAAGTGGAACGTGGACAGAAAATTCAATTTCGGAGAAATTGCTAATTCTCAGGATCCACGTCCACCCGCGAACATTCGCCCCAGCGAATTGAAGCGGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGGTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGCTATATTTCCCGCCCCGGCGGGGAATATGCCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCCTTTGACTTTGGGGGTTTTGGTCCGGCGTTCAGCCGGGACATTACCCCCATGTGCCATGCAGTGAGGGCGTCCAGCCCGAACGTAATGACACACGCCGT
GCCGTATGAATGACATCTATTTCCGTCCAATTCATCGAAACCAGTATTTTGAGGAAATGAAAGCATGATAAAGAAACTTAAAGTCATCATGTTGGCAGCAATAGTTACCGGATGTTCATCGCCGCCGCCTCCCGTTCCTGTGGAGTGGGATAAAGCCGCTGACCCATTAAATACCAGTTTACCGCAATGGAGTGACAATAACGTTATAGTCCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAGTTTCTACGATACAATATGGACTCCAGCAGTATTTTACGCAGTCGCCCATTCAACACGAATTGTCGTCACTGCACAATCCGGCACTGATTTCTTTAATGCCAAAAACTGGCTTCGCCAGAATGGCGCAAAAGGCGTTATTGAATACCAGCCTGTATTTAATTGCCTGACGTGCAGAGAAACCACTATTTATTTCTCTCACCAATCCATTTAAGCAACACAATCTAATAAGGATTTATCATGAAAAAAACCGTTATCGCGGCGACTGTATTATGCCTGACCGCAGGAACGCCGATTTATTCCTTTGCCGCTGACCCGTGCGAAGTTGTTCTTTGCATGTACGGAAAAGCCACAGGTAATAGTGGTGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATCAACGCATTTAAAAAACACCATCGTTTCAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGGGAGTGCAAAGCCGCAGACCCTGAATCAATAAGTAAAATCCTTTCAAAATTTGGCAGAGTGAAAGGATAACTGACATATCCATTTTGATATGAGAGGTTCGAAATGATAAACGAAGATTTATTCATTTAGAATATACACTCAAAAAATCAGGACAGAATATCTGTAGCCATGGGATATGACACGTTATCAAAAGAAGCCCACAGAGGTTGTGGGCTGTATTACGAAATATACGAAAGCTGTTTTATCGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAAGCTGACGCAAATAGATTAAGACGCTATGCCGAGAGTAAAGGTACGAAAATTGATGATGCGTCCTACAGTGAAGCACTGGAAGCTGAAAGGGAGTGCAGGGCTGAAATATACCGCGAGCAAATGTAATCACACAAAACAAAAGTGTATTGTACAATACAGAGACTAACAGGCGACGGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTTCGAAGAAATTGTTAAGACGAAAAATAATGAAGTGGAACGTGGACAGAAAATTCAATTTCGGAGAAATTGCTAATTCTCAGGATCCACGTCCACCCGCGAACATTCGCCCCAGCGAATTGAAGCGGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGGTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGCTATATTTCCCGCCCCGGCGGGGAATATGCCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCCTTTGACTTTGGGGGTTTTGGTCCGGCGTTCAGCCGGGACATTACCCCCATGTGCCATGCAGTGAGGGCGTCCAGCCCGAACGTAATGACACACGCCGT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 821 | GenBank | WP_001096398 |
| Name | t4cp2_UUU_RS00325_pKPt3 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103084.02 Da Isoelectric Point: 7.4625
>WP_001096398.1 MULTISPECIES: VirB3 family type IV secretion system protein [Enterobacterales]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 822 | GenBank | WP_025862121 |
| Name | t4cp2_UUU_RS00300_pKPt3 |
UniProt ID | _ |
| Length | 579 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 579 a.a. Molecular weight: 65593.88 Da Isoelectric Point: 8.0501
>WP_025862121.1 type IV secretion system DNA-binding domain-containing protein [Klebsiella pneumoniae]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRVLAGEIDNLKHRIIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGVTATVLL
YQAILAGEAVFVEDPKDDEWAPHILREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEIFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSHILVKKKHIELLSFENP
QSVQPQPEINEQNKPSINL
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRVLAGEIDNLKHRIIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGVTATVLL
YQAILAGEAVFVEDPKDDEWAPHILREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEIFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSHILVKKKHIELLSFENP
QSVQPQPEINEQNKPSINL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 68649..82167
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| UUU_RS31360 (UUU_25250) | 65183..66109 | + | 927 | WP_025861985 | hypothetical protein | - |
| UUU_RS00340 (UUU_25260) | 66249..66434 | + | 186 | WP_000205185 | AlpA family transcriptional regulator | - |
| UUU_RS28695 (UUU_25270) | 66456..66638 | + | 183 | WP_001513329 | hypothetical protein | - |
| UUU_RS00335 (UUU_25280) | 66623..67204 | + | 582 | WP_000937857 | DUF2857 domain-containing protein | - |
| UUU_RS28700 | 67194..67403 | + | 210 | WP_001112403 | TraR/DksA family transcriptional regulator | - |
| UUU_RS29210 (UUU_25290) | 67625..67774 | + | 150 | Protein_53 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| UUU_RS0126635 | 68343..68576 | + | 234 | WP_001231171 | hypothetical protein | - |
| UUU_RS0126640 | 68649..69359 | + | 711 | WP_001593436 | lytic transglycosylase domain-containing protein | virB1 |
| UUU_RS0126645 | 69359..69652 | + | 294 | WP_000600171 | TrbC/VirB2 family protein | virB2 |
| UUU_RS00325 (UUU_25310) | 69665..72403 | + | 2739 | WP_001096398 | VirB3 family type IV secretion system protein | virb4 |
| UUU_RS00320 (UUU_25320) | 72421..73128 | + | 708 | WP_000857358 | type IV secretion system protein | - |
| UUU_RS28710 (UUU_25330) | 73136..73378 | + | 243 | WP_004150600 | EexN family lipoprotein | - |
| UUU_RS00315 (UUU_25340) | 73382..74455 | + | 1074 | WP_004150601 | type IV secretion system protein | virB6 |
| UUU_RS0126655 | 74677..75360 | + | 684 | WP_016530244 | type IV secretion system protein | virB8 |
| UUU_RS0126660 | 75357..76265 | + | 909 | WP_016530245 | P-type conjugative transfer protein VirB9 | virB9 |
| UUU_RS00310 (UUU_25350) | 76309..77559 | + | 1251 | WP_004150602 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| UUU_RS00305 (UUU_25360) | 77549..78574 | + | 1026 | WP_000125558 | P-type DNA transfer ATPase VirB11 | virB11 |
| UUU_RS28715 (UUU_25370) | 78571..78978 | + | 408 | WP_004150603 | cag pathogenicity island Cag12 family protein | - |
| UUU_RS28720 | 79005..79310 | + | 306 | WP_000754360 | hypothetical protein | - |
| UUU_RS28725 | 79374..79649 | + | 276 | WP_227499860 | dpoa decarboxylase | - |
| UUU_RS00300 (UUU_25410) | 80428..82167 | + | 1740 | WP_025862121 | type IV secretion system DNA-binding domain-containing protein | virb4 |
| UUU_RS00295 (UUU_25420) | 82176..82940 | + | 765 | WP_004150608 | MobC family replication-relaxation protein | - |
| UUU_RS00290 (UUU_25480) | 84209..84721 | - | 513 | WP_000290498 | colibactin self-protection protein ClbS | - |
| UUU_RS0126670 | 84756..85478 | - | 723 | WP_000065645 | colibactin biosynthesis thioesterase ClbQ | - |
| UUU_RS00285 (UUU_25500) | 85471..86985 | - | 1515 | WP_002430641 | precolibactin peptidase ClbP | - |
Host bacterium
| ID | 1682 | GenBank | NZ_AJJI01000025 |
| Plasmid name | pKPt3 | Incompatibility group | - |
| Plasmid size | 103907 bp | Coordinate of oriT [Strand] | 78507..80211 [+] |
| Host baterium | Klebsiella pneumoniae subsp. pneumoniae DSM 30104 = JCM 1662 = NBRC 14940 strain DSM 30104 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, ybtU, ybtT, ybtE, fyuA, clbS, clbQ, clbP, clbO, clbN, clbM, clbL, clbK |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |