Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101236
Name   oriT_pE11210p1 in_silico
Organism   Escherichia coli O104:H4 str. E112/10
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AHAV01000218 (28163..28235 [+], 73 nt)
oriT length   73 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 73 nt

>oriT_pE11210p1
GTCGGGGCAAAGCCCTGACCAGGTGGGGAATGTCTGAGTGCGCGTGCGCGGTCCGACATTCCCACATCCTGTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1210 GenBank   WP_001402881
Name   Relaxase_E11210_RS00130_pE11210p1 insolico UniProt ID   _
Length   903 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 903 a.a.        Molecular weight: 104601.97 Da        Isoelectric Point: 7.5259

>WP_001402881.1 relaxase/mobilization nuclease domain-containing protein [Escherichia coli]
MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDVPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCNSLETAAEEMQYKADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRYFAERLPEMARNGELESCLDVHRTLAKAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGRNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
TTVQCESLIEDMIAGGNFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEL
QAGPFEIAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNADRCVILCEPGGTPFYEDTGVLEARLQKDGS
VRFRDRRNGELVCVDYGDRVVFYHHQDRNELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAW
HNAAGITGNGHFVISRPDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-315)


  Protein structure



No available structure.




Auxiliary protein

ID   425 GenBank   WP_001291058
Name   WP_001291058_pE11210p1 insolico UniProt ID   A0A7I0L241
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12730.52 Da        Isoelectric Point: 9.9656

>WP_001291058.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVSTFIRSSSLDKVVNCRTDDRMIDEIMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITEYVNALRRDLMGR

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A7I0L241


T4CP

ID   820 GenBank   WP_000077664
Name   trbC_E11210_RS00145_pE11210p1 insolico UniProt ID   _
Length   767 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 767 a.a.        Molecular weight: 87040.19 Da        Isoelectric Point: 7.4857

>WP_000077664.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIWPLALPVCLLFTLVTSVIFMLQRWRCP
LRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQYEADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVM
FFATTGGGKTETTFAWLLNPLCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEI
IQSGEKSRPQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSE
TFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFVTQQSMLLARDLGYRLE
GTDAQTLEVKKYKGSFPYICILDEVGAYYTDRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLM
QNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLN
AGEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPEHVSSII
GVLTAKPSRKRRKNRTEPYRIIDTFQRRLATAQTSLDLLPQYDTDIESRANELWKKAVHTINNTTREERR
VCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPDLTASPPHKKNVAQTPSGGKQESRKKRF

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 31..21509

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E11210_RS00260 (E11210_49500) 31..1368 + 1338 WP_001402873 conjugal transfer protein TraO traO
E11210_RS00255 (E11210_49510) 1365..2078 + 714 WP_000787003 conjugal transfer protein TraP traP
E11210_RS00250 (E11210_49520) 2075..2605 + 531 WP_000987021 conjugal transfer protein TraQ traQ
E11210_RS00245 (E11210_49530) 2652..3050 + 399 WP_000088813 DUF6750 family protein traR
E11210_RS32845 (E11210_49540) 3107..3358 + 252 WP_001277573 hypothetical protein -
E11210_RS00235 (E11210_49550) 3273..4091 + 819 WP_000753550 hypothetical protein traT
E11210_RS00230 (E11210_49560) 4386..7430 + 3045 WP_001024766 conjugal transfer protein traU
E11210_RS00225 (E11210_49570) 7430..8050 + 621 WP_000286854 hypothetical protein traV
E11210_RS00220 (E11210_49580) 8008..9213 + 1206 WP_001189548 conjugal transfer protein TraW traW
E11210_RS00215 (E11210_49590) 9210..9779 + 570 WP_000650752 conjugal transfer protein TraX -
E11210_RS00210 (E11210_49600) 9854..12019 + 2166 WP_000691807 DotA/TraY family protein traY
E11210_RS00205 (E11210_49610) 12107..12760 + 654 WP_001402874 plasmid IncI1-type surface exclusion protein ExcA -
E11210_RS00200 (E11210_49620) 13034..14620 + 1587 WP_001402875 TnsA endonuclease N-terminal domain-containing protein -
E11210_RS00195 (E11210_49630) 14758..14910 - 153 WP_001302699 Hok/Gef family protein -
E11210_RS00190 (E11210_49640) 15240..15482 + 243 WP_000650923 hypothetical protein -
E11210_RS00185 (E11210_49650) 15773..16141 + 369 WP_001345852 hypothetical protein -
E11210_RS00180 (E11210_49660) 16145..16360 + 216 WP_000266544 hypothetical protein -
E11210_RS00170 (E11210_49680) 16558..17181 - 624 WP_001155248 helix-turn-helix domain-containing protein -
E11210_RS00165 (E11210_49690) 17341..17808 + 468 WP_000598962 thermonuclease family protein -
E11210_RS32220 (E11210_49700) 17959..18135 + 177 WP_001054907 hypothetical protein -
E11210_RS32850 18198..18293 - 96 WP_001363141 DinQ-like type I toxin DqlB -
E11210_RS00155 (E11210_49710) 19090..20388 + 1299 WP_001402878 IncI1-type conjugal transfer protein TrbA trbA
E11210_RS00150 (E11210_49720) 20385..21509 + 1125 WP_000152387 DsbC family protein trbB
E11210_RS00145 (E11210_49730) 21490..23793 + 2304 WP_000077664 F-type conjugative transfer protein TrbC -
E11210_RS30045 24260..24490 + 231 WP_065406859 hypothetical protein -
E11210_RS00135 (E11210_49760) 24588..25019 - 432 WP_001335086 cell envelope integrity TolA C-terminal domain-containing protein -

Host bacterium

ID   1680 GenBank   NZ_AHAV01000218
Plasmid name   pE11210p1 Incompatibility group   -
Plasmid size   45938 bp Coordinate of oriT [Strand]   28163..28235 [+]
Host baterium   Escherichia coli O104:H4 str. E112/10

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -