Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101233 |
| Name | oriT_p56  |
| Organism | Escherichia coli O157:H7 strain 438/99 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JAHCTZ010000004 (12818..12870 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_p56
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 819 | GenBank | WP_000338972 |
| Name | t4cp2_JKN66_RS27820_p56 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73329.86 Da Isoelectric Point: 9.1391
>WP_000338972.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDISLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDISLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 29455..51670
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JKN66_RS27765 (JKN66_27560) | 24780..25433 | - | 654 | WP_000572550 | hypothetical protein | - |
| JKN66_RS27770 (JKN66_27565) | 25445..26569 | - | 1125 | WP_000486720 | site-specific integrase | - |
| JKN66_RS28720 (JKN66_27570) | 26982..27203 | - | 222 | Protein_37 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| JKN66_RS27775 (JKN66_27575) | 27517..28803 | - | 1287 | WP_015057162 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| JKN66_RS27780 (JKN66_27580) | 28816..29451 | - | 636 | WP_000934978 | A24 family peptidase | - |
| JKN66_RS27785 (JKN66_27585) | 29455..29937 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| JKN66_RS27790 (JKN66_27590) | 30004..30561 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| JKN66_RS27795 (JKN66_27595) | 30606..31715 | - | 1110 | WP_226280623 | type II secretion system F family protein | - |
| JKN66_RS27800 (JKN66_27600) | 31706..33244 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| JKN66_RS27805 (JKN66_27605) | 33269..33763 | - | 495 | WP_000912554 | type IV pilus biogenesis protein PilP | - |
| JKN66_RS27810 (JKN66_27610) | 33747..35057 | - | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| JKN66_RS27815 (JKN66_27615) | 35108..36751 | - | 1644 | WP_001035589 | PilN family type IVB pilus formation outer membrane protein | - |
| JKN66_RS27820 (JKN66_27620) | 36802..38760 | - | 1959 | WP_000338972 | type IV secretory system conjugative DNA transfer family protein | - |
| JKN66_RS27825 (JKN66_27625) | 38776..39831 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| JKN66_RS27830 (JKN66_27630) | 39882..41021 | - | 1140 | WP_000790639 | TrbI/VirB10 family protein | virB10 |
| JKN66_RS27835 (JKN66_27635) | 41011..41712 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| JKN66_RS27840 (JKN66_27640) | 41778..42512 | - | 735 | WP_000432283 | type IV secretion system protein | virB8 |
| JKN66_RS27845 (JKN66_27645) | 42678..45035 | - | 2358 | WP_000548951 | VirB4 family type IV secretion system protein | virb4 |
| JKN66_RS27850 (JKN66_27650) | 45041..45361 | - | 321 | WP_000362083 | VirB3 family type IV secretion system protein | virB3 |
| JKN66_RS27855 (JKN66_27655) | 45432..45722 | - | 291 | WP_000865478 | TrbC/VirB2 family protein | virB2 |
| JKN66_RS27860 (JKN66_27660) | 45722..46306 | - | 585 | WP_001177114 | lytic transglycosylase domain-containing protein | virB1 |
| JKN66_RS27865 (JKN66_27665) | 46327..46725 | - | 399 | WP_001153669 | hypothetical protein | - |
| JKN66_RS27870 (JKN66_27670) | 46844..47281 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| JKN66_RS27875 (JKN66_27675) | 47287..48522 | - | 1236 | WP_000733393 | toxin co-regulated pilus biosynthesis Q family protein | - |
| JKN66_RS27880 (JKN66_27680) | 48525..48812 | - | 288 | WP_001326593 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| JKN66_RS27885 (JKN66_27685) | 48984..49619 | - | 636 | WP_022645136 | hypothetical protein | - |
| JKN66_RS27890 (JKN66_27690) | 49772..50026 | - | 255 | WP_001043555 | EexN family lipoprotein | - |
| JKN66_RS27895 (JKN66_27695) | 50028..50669 | - | 642 | WP_001463086 | type IV secretion system protein | - |
| JKN66_RS27900 (JKN66_27700) | 50675..51670 | - | 996 | WP_001028543 | type IV secretion system protein | virB6 |
| JKN66_RS27905 (JKN66_27705) | 51674..51931 | - | 258 | WP_032213114 | hypothetical protein | - |
| JKN66_RS27910 (JKN66_27710) | 51928..52230 | - | 303 | WP_001360345 | hypothetical protein | - |
| JKN66_RS27915 (JKN66_27715) | 52211..52468 | - | 258 | WP_001542015 | hypothetical protein | - |
| JKN66_RS27920 (JKN66_27720) | 52502..53155 | - | 654 | WP_044069354 | hypothetical protein | - |
| JKN66_RS27925 (JKN66_27725) | 53166..53336 | - | 171 | WP_044069355 | hypothetical protein | - |
| JKN66_RS27930 (JKN66_27730) | 53340..53783 | - | 444 | WP_072019033 | NfeD family protein | - |
| JKN66_RS27935 (JKN66_27735) | 54163..55116 | - | 954 | WP_072097371 | SPFH domain-containing protein | - |
| JKN66_RS27940 (JKN66_27740) | 55162..55356 | - | 195 | WP_001127358 | DUF1187 family protein | - |
| JKN66_RS27945 (JKN66_27745) | 55349..55801 | - | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 1677 | GenBank | NZ_JAHCTZ010000004 |
| Plasmid name | p56 | Incompatibility group | IncI2 |
| Plasmid size | 56932 bp | Coordinate of oriT [Strand] | 12818..12870 [-] |
| Host baterium | Escherichia coli O157:H7 strain 438/99 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |