Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101225
Name   oriT_pEnA16186_KPC2 in_silico
Organism   Klebsiella aerogenes strain EnA16186
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_VKNT01000070 (19363..19462 [-], 100 nt)
oriT length   100 nt
IRs (inverted repeats)      77..82, 90..95  (AAAAAA..TTTTTT)
 78..83, 90..95  (AAAAAA..TTTTTT)
 79..84, 90..95  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 100 nt

>oriT_pEnA16186_KPC2
TATTTATTTTTTTATCTTTTAAATCAGTACGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGAAAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1205 GenBank   WP_012561142
Name   mobF_FOT90_RS25110_pEnA16186_KPC2 insolico UniProt ID   A0A1S6KKJ9
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120195.37 Da        Isoelectric Point: 6.5230

>WP_012561142.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKADMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

AAA domain (AAA_30)

(477-653)

TrwC relaxase (TrwC)

(16-287)


  Protein structure


Source ID Structure
AlphaFold DB A0A1S6KKJ9


Auxiliary protein

ID   421 GenBank   WP_001749975
Name   WP_001749975_pEnA16186_KPC2 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   814 GenBank   WP_000342688
Name   t4cp2_FOT90_RS25105_pEnA16186_KPC2 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 41490..51819

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOT90_RS25190 (FOT90_25920) 37465..38325 + 861 WP_000027057 broad-spectrum class A beta-lactamase TEM-1 -
FOT90_RS25195 (FOT90_25925) 38584..39465 + 882 WP_004199234 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -
FOT90_RS25200 (FOT90_25930) 39715..41034 - 1320 WP_004152397 IS1182-like element ISKpn6 family transposase -
FOT90_RS25205 41386..41490 - 105 Protein_42 phospholipase D family protein -
FOT90_RS25210 (FOT90_25940) 41490..42485 - 996 WP_012561144 ATPase, T2SS/T4P/T4SS family virB11
FOT90_RS25215 (FOT90_25945) 42527..43687 - 1161 WP_000101710 type IV secretion system protein VirB10 virB10
FOT90_RS25220 (FOT90_25950) 43687..44571 - 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
FOT90_RS25225 (FOT90_25955) 44582..45280 - 699 WP_000646594 type IV secretion system protein virB8
FOT90_RS25230 (FOT90_25965) 45499..46539 - 1041 WP_001749958 type IV secretion system protein virB6
FOT90_RS25235 (FOT90_25970) 46555..46782 - 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
FOT90_RS25240 (FOT90_25975) 46790..47503 - 714 WP_001749960 type IV secretion system protein virB5
FOT90_RS25245 (FOT90_25980) 47521..50121 - 2601 WP_012561149 VirB4 family type IV secretion/conjugal transfer ATPase virb4
FOT90_RS25250 (FOT90_25985) 50121..50438 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
FOT90_RS25255 (FOT90_25990) 50489..50782 - 294 WP_001749962 hypothetical protein virB2
FOT90_RS25260 (FOT90_25995) 50792..51073 - 282 WP_016338364 transcriptional repressor KorA -
FOT90_RS25265 (FOT90_26000) 51082..51819 - 738 WP_013279384 lytic transglycosylase domain-containing protein virB1
FOT90_RS25270 (FOT90_26005) 51928..52233 + 306 WP_012561154 H-NS family nucleoid-associated regulatory protein -
FOT90_RS25275 (FOT90_26010) 52249..52593 + 345 WP_012561155 hypothetical protein -
FOT90_RS25280 (FOT90_26015) 52590..52904 + 315 WP_016338363 TrbM/KikA/MpfK family conjugal transfer protein -
FOT90_RS25285 (FOT90_26020) 53030..53251 + 222 WP_223862372 hypothetical protein -
FOT90_RS25575 53307..53567 + 261 WP_016359294 hypothetical protein -
FOT90_RS25295 (FOT90_26030) 53609..54577 - 969 WP_016151349 IS5 family transposase -
FOT90_RS25300 (FOT90_26035) 54661..55014 + 354 WP_225622145 restriction endonuclease -
FOT90_RS25305 (FOT90_26040) 55019..55225 + 207 WP_001749967 hypothetical protein -
FOT90_RS25310 (FOT90_26045) 55236..55508 - 273 Protein_63 IS1 family transposase -

Host bacterium

ID   1669 GenBank   NZ_VKNT01000070
Plasmid name   pEnA16186_KPC2 Incompatibility group   IncN
Plasmid size   58447 bp Coordinate of oriT [Strand]   19363..19462 [-]
Host baterium   Klebsiella aerogenes strain EnA16186

Cargo genes

Drug resistance gene   aph(6)-Id, aph(3'')-Ib, aac(3)-IId, blaTEM-1B, blaKPC-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -