Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 101223 |
Name | oriT_pSS20EcIncN |
Organism | Escherichia coli strain EcSS20 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_SDDW01000035 (3089..3189 [-], 101 nt) |
oriT length | 101 nt |
IRs (inverted repeats) | 80..85, 91..96 (AAAAAA..TTTTTT) 20..26, 38..44 (TAAATCA..TGATTTA) |
Location of nic site | 62..63 |
Conserved sequence flanking the nic site |
GGTGTATAGC |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 101 nt
>oriT_pSS20EcIncN
TATTTATTTTTTTATCTTTTAAATCAGCATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG
TATTTATTTTTTTATCTTTTAAATCAGCATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 1204 | GenBank | WP_129015033 |
Name | mobF_ERL57_RS23110_pSS20EcIncN | UniProt ID | _ |
Length | 1078 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 1078 a.a. Molecular weight: 120114.30 Da Isoelectric Point: 6.5512
>WP_129015033.1 MobF family relaxase [Escherichia coli]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RNVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RNVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
ID | 420 | GenBank | WP_000706094 |
Name | WP_000706094_pSS20EcIncN | UniProt ID | B6UZ32 |
Length | 96 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 96 a.a. Molecular weight: 10829.54 Da Isoelectric Point: 8.5040
>WP_000706094.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MKIVADRLSDVNRKLDYLFDRASDADFGPLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIA
EPGKTKAAKAEVERNGYKVWEPKKER
MKIVADRLSDVNRKLDYLFDRASDADFGPLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIA
EPGKTKAAKAEVERNGYKVWEPKKER
Protein domains
No domain identified.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | B6UZ32 |
T4CP
ID | 813 | GenBank | WP_000342688 |
Name | t4cp2_ERL57_RS23105_pSS20EcIncN | UniProt ID | _ |
Length | 509 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 509 a.a. Molecular weight: 57762.87 Da Isoelectric Point: 9.6551
>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 3756..20043
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
ERL57_RS23075 (ERL57_23075) | 103..282 | + | 180 | WP_012561134 | protein CcgAI | - |
ERL57_RS23080 (ERL57_23080) | 744..1088 | - | 345 | WP_012561137 | hypothetical protein | - |
ERL57_RS23085 (ERL57_23085) | 1289..1657 | - | 369 | WP_012561138 | plasmid stabilization protein StbC | - |
ERL57_RS23090 (ERL57_23090) | 1659..2375 | - | 717 | WP_012561139 | StbB family protein | - |
ERL57_RS23095 (ERL57_23095) | 2384..2770 | - | 387 | WP_013279398 | plasmid stabilization protein StbA | - |
ERL57_RS23100 (ERL57_23100) | 3464..3754 | + | 291 | WP_000706094 | hypothetical protein | - |
ERL57_RS23105 (ERL57_23105) | 3756..5285 | + | 1530 | WP_000342688 | type IV secretion system DNA-binding domain-containing protein | virb4 |
ERL57_RS23110 (ERL57_23110) | 5285..8521 | + | 3237 | WP_129015033 | MobF family relaxase | - |
ERL57_RS23115 (ERL57_23115) | 8521..9147 | + | 627 | WP_013851369 | DUF6710 family protein | - |
ERL57_RS23120 (ERL57_23120) | 9320..9853 | - | 534 | WP_129015034 | phospholipase D family protein | - |
ERL57_RS23125 (ERL57_23125) | 9853..10848 | - | 996 | WP_129015035 | ATPase, T2SS/T4P/T4SS family | virB11 |
ERL57_RS23130 (ERL57_23130) | 10890..12050 | - | 1161 | WP_033547518 | type IV secretion system protein VirB10 | virB10 |
ERL57_RS23135 (ERL57_23135) | 12050..12934 | - | 885 | WP_163562551 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
ERL57_RS23140 (ERL57_23140) | 12945..13643 | - | 699 | WP_000646594 | type IV secretion system protein | virB8 |
ERL57_RS23145 (ERL57_23145) | 13633..13779 | - | 147 | WP_001257173 | conjugal transfer protein TraN | - |
ERL57_RS23150 (ERL57_23150) | 13862..14902 | - | 1041 | WP_001749958 | type IV secretion system protein | virB6 |
ERL57_RS23155 (ERL57_23155) | 14918..15145 | - | 228 | WP_001749959 | IncN-type entry exclusion lipoprotein EexN | - |
ERL57_RS23160 (ERL57_23160) | 15153..15866 | - | 714 | WP_113472287 | type IV secretion system protein | virB5 |
ERL57_RS23165 (ERL57_23165) | 15884..18484 | - | 2601 | WP_012561149 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
ERL57_RS23170 (ERL57_23170) | 18484..18801 | - | 318 | WP_000496058 | VirB3 family type IV secretion system protein | virB3 |
ERL57_RS23175 (ERL57_23175) | 18851..19144 | - | 294 | WP_001749962 | hypothetical protein | virB2 |
ERL57_RS23180 (ERL57_23180) | 19154..19435 | - | 282 | WP_032335136 | transcriptional repressor KorA | - |
ERL57_RS23185 (ERL57_23185) | 19444..20043 | - | 600 | WP_049067177 | lytic transglycosylase domain-containing protein | virB1 |
ERL57_RS23190 (ERL57_23190) | 20221..20592 | + | 372 | WP_011867773 | H-NS family nucleoid-associated regulatory protein | - |
ERL57_RS23195 (ERL57_23195) | 20602..20952 | + | 351 | WP_250188724 | hypothetical protein | - |
ERL57_RS23200 (ERL57_23200) | 20949..21263 | + | 315 | WP_113472288 | TrbM/KikA/MpfK family conjugal transfer protein | - |
ERL57_RS23205 (ERL57_23205) | 21299..21610 | + | 312 | WP_001452736 | hypothetical protein | - |
ERL57_RS23210 (ERL57_23210) | 21660..22313 | + | 654 | WP_113472289 | restriction endonuclease | - |
ERL57_RS23215 (ERL57_23215) | 22314..22532 | + | 219 | WP_113472290 | hypothetical protein | - |
ERL57_RS23220 (ERL57_23220) | 22532..22714 | + | 183 | WP_071884977 | Hha/YmoA family nucleoid-associated regulatory protein | - |
ERL57_RS23225 (ERL57_23225) | 23014..23583 | + | 570 | WP_001749988 | recombinase family protein | - |
Host bacterium
ID | 1667 | GenBank | NZ_SDDW01000035 |
Plasmid name | pSS20EcIncN | Incompatibility group | - |
Plasmid size | 28224 bp | Coordinate of oriT [Strand] | 3089..3189 [-] |
Host baterium | Escherichia coli strain EcSS20 |
Cargo genes
Drug resistance gene | - |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |