Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 101128
• Name: oriT_pSauR65-1
• Organism: Staphylococcus aureus strain SauR65
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_JAIUJL010000023 (23693..23837 [-], 145 nt)
• oriT length: 145 nt
• IRs (inverted repeats): 105..110, 114..119 (TTTAAA..TTTAAA) 64..71, 76..83 (GTGTCACA..TGTGACAC) 33..39, 44..50 (TGTCACA..TGTGACA)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 145 nt

>oriT_pSauR65-1
TGTCACAAAAATAAGACAAATTCTACATGTTGTGTCACAAAACTGTGACAAACACAATATATAGTGTCACAAAACTGTGACACCATAGTTTATTGTGGGGTCACTTTAAATTCTTTAAAAACCTTGAAATGGCTGGCTTTGCCAG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobP2_LCD06_RS12900_pSauR65-1

• ID: 1138
• GenBank: WP_223224015
• Name: mobP2_LCD06_RS12900_pSauR65-1
• UniProt ID: _
• Length: 383 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 383 a.a.
• Molecular weight: 45870.14 Da
• Isoelectric Point: 9.9348

>WP_223224015.1 MobP2 family relaxase [Staphylococcus aureus]
MMPKIVTVSQFEQPNEFYEYSNYVNYMKRGEAQNNKDDFKYDVYAHYMFDEEKSQNMFNEHDNFMSKKAI
NKIKSDFSHAQKNKGMMWKDVISFDNSALESVGIYDSKTHTLDEQKIKEATRKMMKRFEKKEGLEDNLVW
SGAIHYNTDNIHVHVAAVEKVIKRTRGKRKPATLKHMKSTFANELFDIKGERQKINEFIRERIVKGIRDE
SEPYKDKAMKKQIKKVHKLLEDIPRNQWNYNNNILKYIRPEIDKITDIYIKNYHSKDFEVFKNDLKRQTN
LYKETYGENSNYGSYEKTKMKDLYARSGNTILKNIKELDQDLMKVKQQSTHSKSNATITKLKIGKCLNQA
LYRTKYALRSDYDKEKNIQQYYQEFDKQSYRER

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (2-358)

Protein structure

No available structure.

T4CP

t4cp2_LCD06_RS12950_pSauR65-1

• ID: 700
• GenBank: WP_012816493
• Name: t4cp2_LCD06_RS12950_pSauR65-1
• UniProt ID: _
• Length: 924 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 924 a.a.
• Molecular weight: 106377.32 Da
• Isoelectric Point: 7.5736

>WP_012816493.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Staphylococcus]
MRFGSKDKHGYRRKKLSFSQNKHLLAHPCILVSILLLIMLLATAIANFLINILMTVFFGNHDIFTNMKQL
NTKYTDYIFNFRLDAVLLYIPIWVLVFIFFGKKVYQFRQRFKSLNNNEKASGRFATRKEITQQYKAIPQR
EHAFEGEGGLPVAMFGVYDFVTKTKQYLEQRKTAIREINGITDLTADEKLELRKDKLGKMNKEFATTFFS
KREFTFIDESPTNNLIVGTSRSGKGEIVVLSMIENYSRSSEQPSLIVNDMKGELFSASYKTLENRGYQVE
IFNLDQPMESTMSFNPLQQVIDEYMKGDLGEAQEMTKQITYTLTNNEGVEDNEWNLMAGALINAMILALC
EETLPKNPEKVTLYSVSNMLQTLSTKRFYKEIAIGNKVQLIEVSALDEYMERFPSHSPAKTQYATVQAAP
DKMRSSIIGTALKALQPFTTDTIAKLTSHTSFDINKLGFPSIIDGYATKDSTFELGVQVLRDGENGIEYE
EVEGIQIGVNEYGYWQYPFKTVLKVGDRIETIEKDGNNQVITSYFYVDHIDDKGQVTFNQKGELDNSDIT
IRKFNSFPKPIAVFMVVPDYNNANHGIASILVNQIVFEISKNSQLYTENQSTYRRVIFHLDEAGNMPPIP
NLSQKVNVSLSRGLRFNFFVQAFSQFKDKYGDAYDAIMDACQNKVYIMATQEQTLKDFSAMIGSQQITVH
SRSGEAGAIKSSITENQEERPLLRPDELARLQEGETVVVRNLKRQDKKRNKVMSYPIFNDGKYAMKYRYQ
YLSDLMDTSQSIIHFRPMLKARCEHRHLQLEATLVDWDKRIEEMQEGIDGQKEQENKNINDINQYKQSNE
EGNVAVRVKRLETLKEKVGDAIFDKITRRFERYLRTYAEQGKNVNTITQKKLEELVSADNEVKEEEKTKR
IEMIQKFIKEAKTT

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (615-732)

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (209-458)

Protein structure

No available structure.

Host bacterium

• ID: 1572
• GenBank: NZ_JAIUJL010000023
• Plasmid name: pSauR65-1
• Incompatibility group: -
• Plasmid size: 39652 bp
• Coordinate of oriT [Strand]: 23693..23837 [-]
• Host baterium: Staphylococcus aureus strain SauR65

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA14, AcrIIA21