Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101120
Name   oriT_p3122-10 in_silico
Organism   Klebsiella pneumoniae strain FK3122
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAOVSH010000010 (42472..42553 [+], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_p3122-10
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1135 GenBank   WP_032440569
Name   traI_OCJ22_RS17435_p3122-10 insolico UniProt ID   A0A7G3L4G1
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74384.24 Da        Isoelectric Point: 8.3203

>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB A0A7G3L4G1


T4CP

ID   696 GenBank   WP_032440593
Name   trbC_OCJ22_RS17570_p3122-10 insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 82679.78 Da        Isoelectric Point: 6.3201

>WP_032440593.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 51662..73595

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OCJ22_RS17445 (OCJ22_17445) 46809..47303 - 495 WP_032440571 molybdopterin-dependent oxidoreductase -
OCJ22_RS17450 (OCJ22_17450) 47296..48225 - 930 WP_032440581 hypothetical protein -
OCJ22_RS17455 (OCJ22_17455) 48285..49127 - 843 WP_032440572 helix-turn-helix domain-containing protein -
OCJ22_RS17460 (OCJ22_17460) 49737..49964 + 228 WP_032440573 hypothetical protein -
OCJ22_RS17465 (OCJ22_17465) 50696..51175 + 480 WP_032440574 lytic transglycosylase domain-containing protein -
OCJ22_RS17470 (OCJ22_17470) 51201..51665 + 465 WP_225546581 DotD/TraH family lipoprotein -
OCJ22_RS17475 (OCJ22_17475) 51662..52480 + 819 WP_032440576 type IV secretory system conjugative DNA transfer family protein traI
OCJ22_RS17480 (OCJ22_17480) 52458..53645 + 1188 WP_077255878 plasmid transfer ATPase TraJ virB11
OCJ22_RS17485 (OCJ22_17485) 53642..53908 + 267 WP_032440577 IcmT/TraK family protein traK
OCJ22_RS17490 (OCJ22_17490) 53926..57339 + 3414 WP_052145516 LPD7 domain-containing protein -
OCJ22_RS17495 (OCJ22_17495) 57373..57726 + 354 WP_032440584 hypothetical protein traL
OCJ22_RS17500 (OCJ22_17500) 57798..58466 + 669 WP_032440585 DotI/IcmL/TraM family protein traM
OCJ22_RS17505 (OCJ22_17505) 58477..59580 + 1104 WP_077264204 DotH/IcmK family type IV secretion protein traN
OCJ22_RS17510 (OCJ22_17510) 59584..60924 + 1341 WP_032440586 conjugal transfer protein TraO traO
OCJ22_RS17515 (OCJ22_17515) 60931..61650 + 720 WP_050597333 conjugal transfer protein TraP traP
OCJ22_RS17520 (OCJ22_17520) 61660..62193 + 534 WP_032440587 conjugal transfer protein TraQ traQ
OCJ22_RS17525 (OCJ22_17525) 62249..62650 + 402 WP_032440588 DUF6750 family protein traR
OCJ22_RS17530 (OCJ22_17530) 62694..63257 + 564 WP_045325070 hypothetical protein traT
OCJ22_RS17535 (OCJ22_17535) 63267..66314 + 3048 WP_032440589 conjugal transfer protein traU
OCJ22_RS17540 (OCJ22_17540) 66324..67520 + 1197 WP_032440590 conjugal transfer protein TraW traW
OCJ22_RS17545 (OCJ22_17545) 67517..68098 + 582 WP_050597335 conjugal transfer protein TraX -
OCJ22_RS17550 (OCJ22_17550) 68140..70284 + 2145 WP_032440591 DotA/TraY family protein traY
OCJ22_RS17555 (OCJ22_17555) 70350..70973 + 624 WP_032440592 plasmid IncI1-type surface exclusion protein ExcA -
OCJ22_RS17560 (OCJ22_17560) 71075..72493 + 1419 WP_063666705 hypothetical protein trbA
OCJ22_RS17565 (OCJ22_17565) 72504..73595 + 1092 WP_050597336 thioredoxin fold domain-containing protein trbB
OCJ22_RS17570 (OCJ22_17570) 73597..75789 + 2193 WP_032440593 F-type conjugative transfer protein TrbC -
OCJ22_RS17575 (OCJ22_17575) 75835..76368 + 534 WP_032440594 phospholipase D family protein -
OCJ22_RS17580 (OCJ22_17580) 76380..76631 + 252 WP_113394424 hypothetical protein -
OCJ22_RS17585 (OCJ22_17585) 77137..77832 + 696 WP_254910806 RepA family replication protein -

Host bacterium

ID   1564 GenBank   NZ_JAOVSH010000010
Plasmid name   p3122-10 Incompatibility group   IncFII
Plasmid size   112567 bp Coordinate of oriT [Strand]   42472..42553 [+]
Host baterium   Klebsiella pneumoniae strain FK3122

Cargo genes

Drug resistance gene   blaNDM-5, sul1, qacE, aadA2, tet(A), blaLAP-2, qnrS1, dfrA14, sul2, catA2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -