Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101113 |
| Name | oriT_p14005RM  |
| Organism | Escherichia coli strain 14005RM |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JAAWUF020000023 (13136..13188 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_p14005RM
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTACCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTACCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 694 | GenBank | WP_015059539 |
| Name | t4cp2_HFK70_RS25665_p14005RM |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 35463..60231
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HFK70_RS25600 (HFK70_025620) | 30952..32076 | - | 1125 | WP_000486716 | site-specific integrase | - |
| HFK70_RS25945 | 32489..32710 | - | 222 | Protein_39 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HFK70_RS25605 (HFK70_025625) | 32981..33136 | - | 156 | WP_001358489 | hypothetical protein | - |
| HFK70_RS25610 (HFK70_025630) | 33145..33516 | + | 372 | WP_172690083 | pilus assembly protein | - |
| HFK70_RS25615 (HFK70_025635) | 33495..34811 | - | 1317 | WP_021500066 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HFK70_RS25620 (HFK70_025640) | 34824..35459 | - | 636 | WP_000934977 | A24 family peptidase | - |
| HFK70_RS25625 (HFK70_025645) | 35463..35945 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| HFK70_RS25630 (HFK70_025650) | 36011..36568 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| HFK70_RS25635 (HFK70_025655) | 36613..37722 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| HFK70_RS25640 (HFK70_025660) | 37713..39251 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HFK70_RS25645 (HFK70_025665) | 39276..39770 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| HFK70_RS25650 (HFK70_025670) | 39754..41064 | - | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| HFK70_RS25655 (HFK70_025675) | 41115..42758 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| HFK70_RS25660 (HFK70_025680) | 42751..43287 | - | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| HFK70_RS25665 (HFK70_025685) | 43334..45292 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| HFK70_RS25670 (HFK70_025690) | 45308..46363 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| HFK70_RS25675 (HFK70_025695) | 46382..47521 | - | 1140 | WP_000790640 | TrbI/VirB10 family protein | virB10 |
| HFK70_RS25680 (HFK70_025700) | 47654..48864 | + | 1211 | Protein_55 | IS3 family transposase | - |
| HFK70_RS25685 (HFK70_025705) | 48867..49523 | - | 657 | WP_317619445 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| HFK70_RS25690 (HFK70_025710) | 49589..50323 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| HFK70_RS25695 (HFK70_025715) | 50489..52846 | - | 2358 | WP_063078682 | VirB4 family type IV secretion system protein | virb4 |
| HFK70_RS25700 (HFK70_025720) | 52852..53172 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| HFK70_RS25705 (HFK70_025725) | 53243..53533 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| HFK70_RS25710 (HFK70_025730) | 53533..54117 | - | 585 | WP_001177113 | lytic transglycosylase domain-containing protein | virB1 |
| HFK70_RS25715 (HFK70_025735) | 54138..54536 | - | 399 | WP_001153665 | hypothetical protein | - |
| HFK70_RS25720 (HFK70_025740) | 54655..55092 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| HFK70_RS25725 (HFK70_025745) | 55098..56333 | - | 1236 | WP_015059538 | TcpQ domain-containing protein | - |
| HFK70_RS25730 (HFK70_025750) | 56336..56635 | - | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HFK70_RS25735 (HFK70_025755) | 56703..56984 | - | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HFK70_RS25740 (HFK70_025760) | 56974..57225 | - | 252 | WP_000121741 | hypothetical protein | - |
| HFK70_RS25745 (HFK70_025765) | 57325..57960 | - | 636 | WP_015059536 | hypothetical protein | - |
| HFK70_RS25750 (HFK70_025770) | 58033..58320 | - | 288 | WP_001032611 | EexN family lipoprotein | - |
| HFK70_RS25755 (HFK70_025775) | 58333..58587 | - | 255 | WP_001043555 | EexN family lipoprotein | - |
| HFK70_RS25760 (HFK70_025780) | 58589..59230 | - | 642 | WP_001425343 | type IV secretion system protein | - |
| HFK70_RS25765 (HFK70_025785) | 59236..60231 | - | 996 | WP_001028543 | type IV secretion system protein | virB6 |
| HFK70_RS25770 (HFK70_025790) | 60235..60492 | - | 258 | WP_000739144 | hypothetical protein | - |
| HFK70_RS25775 (HFK70_025795) | 60489..60791 | - | 303 | WP_001360345 | hypothetical protein | - |
| HFK70_RS25780 (HFK70_025800) | 60772..61029 | - | 258 | WP_001542015 | hypothetical protein | - |
| HFK70_RS25785 (HFK70_025805) | 61062..61508 | - | 447 | WP_001243165 | hypothetical protein | - |
| HFK70_RS25790 (HFK70_025810) | 61519..61689 | - | 171 | WP_000550720 | hypothetical protein | - |
| HFK70_RS25795 (HFK70_025815) | 61693..62136 | - | 444 | WP_000964330 | NfeD family protein | - |
| HFK70_RS25800 (HFK70_025820) | 62510..63463 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| HFK70_RS25805 (HFK70_025825) | 63490..63666 | - | 177 | WP_000753050 | hypothetical protein | - |
| HFK70_RS25810 (HFK70_025830) | 63659..63874 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| HFK70_RS25815 (HFK70_025835) | 63867..64319 | - | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 1557 | GenBank | NZ_JAAWUF020000023 |
| Plasmid name | p14005RM | Incompatibility group | IncI2 |
| Plasmid size | 65458 bp | Coordinate of oriT [Strand] | 13136..13188 [-] |
| Host baterium | Escherichia coli strain 14005RM |
Cargo genes
| Drug resistance gene | mcr-1.5 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |