Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101111
Name   oriT_IncC in_silico
Organism   Enterobacter hormaechei subsp. xiangfangensis strain PLCR20
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAGRZM010000011 (52322..52472 [-], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_IncC
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1130 GenBank   WP_001326173
Name   mobH_KC310_RS20300_IncC insolico UniProt ID   F5BPR1
Length   990 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB F5BPR1


T4CP

ID   692 GenBank   WP_000637384
Name   traC_KC310_RS20215_IncC insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92604.86 Da        Isoelectric Point: 5.9216

>WP_000637384.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSQGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRRQAA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

TraG P-loop domain (P-loop_TraG)

(449-809)

  Protein structure



No available structure.



ID   693 GenBank   WP_000178857
Name   traD_KC310_RS20295_IncC insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69225.71 Da        Isoelectric Point: 6.9403

>WP_000178857.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKVKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-299)

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 29245..54388

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KC310_RS20155 (KC310_20155) 24371..25279 - 909 WP_000739139 hypothetical protein -
KC310_RS20160 (KC310_20160) 25290..26258 - 969 WP_000085160 AAA family ATPase -
KC310_RS20165 (KC310_20165) 26478..26663 - 186 WP_001186917 hypothetical protein -
KC310_RS20170 (KC310_20170) 26967..27368 - 402 WP_286117583 hypothetical protein -
KC310_RS20175 (KC310_20175) 27582..28223 + 642 WP_000796664 hypothetical protein -
KC310_RS20180 (KC310_20180) 28346..29206 + 861 WP_000709517 hypothetical protein -
KC310_RS20185 (KC310_20185) 29245..32040 - 2796 WP_001256484 conjugal transfer mating pair stabilization protein TraN traN
KC310_RS20190 (KC310_20190) 32156..33160 - 1005 WP_005507569 TraU family protein traU
KC310_RS20195 (KC310_20195) 33160..33831 - 672 WP_000575345 EAL domain-containing protein -
KC310_RS20200 (KC310_20200) 33828..35093 - 1266 WP_001447719 TrbC family F-type conjugative pilus assembly protein traW
KC310_RS20205 (KC310_20205) 35056..35586 - 531 WP_001010740 S26 family signal peptidase -
KC310_RS20210 (KC310_20210) 35583..35900 - 318 WP_000351984 hypothetical protein -
KC310_RS20215 (KC310_20215) 35915..38362 - 2448 WP_000637384 type IV secretion system protein TraC virb4
KC310_RS20220 (KC310_20220) 38359..39066 - 708 WP_001259346 DsbC family protein -
KC310_RS20225 (KC310_20225) 39215..44701 - 5487 WP_000606835 Ig-like domain-containing protein -
KC310_RS20230 (KC310_20230) 44902..45294 - 393 WP_000479535 TraA family conjugative transfer protein -
KC310_RS20235 (KC310_20235) 45298..45876 - 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
KC310_RS20240 (KC310_20240) 45873..47186 - 1314 WP_024131605 TraB/VirB10 family protein traB
KC310_RS20245 (KC310_20245) 47186..48103 - 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
KC310_RS20250 (KC310_20250) 48087..48713 - 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
KC310_RS20255 (KC310_20255) 48710..48991 - 282 WP_000805625 type IV conjugative transfer system protein TraL traL
KC310_RS20260 (KC310_20260) 49136..49501 - 366 WP_001052530 hypothetical protein -
KC310_RS20265 (KC310_20265) 49513..49656 - 144 WP_001275801 hypothetical protein -
KC310_RS20270 (KC310_20270) 49837..50499 - 663 WP_001231464 hypothetical protein -
KC310_RS20275 (KC310_20275) 50499..50876 - 378 WP_000869297 hypothetical protein -
KC310_RS20280 (KC310_20280) 50886..51332 - 447 WP_000122507 hypothetical protein -
KC310_RS20285 (KC310_20285) 51342..51971 - 630 WP_000743449 DUF4400 domain-containing protein tfc7
KC310_RS20290 (KC310_20290) 51928..52473 - 546 WP_000228720 hypothetical protein -
KC310_RS20295 (KC310_20295) 52523..54388 - 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
KC310_RS20300 (KC310_20300) 54385..57357 - 2973 WP_001326173 MobH family relaxase -
KC310_RS20305 (KC310_20305) 57525..58142 + 618 WP_001249395 hypothetical protein -
KC310_RS20310 (KC310_20310) 58124..58357 + 234 WP_001191890 hypothetical protein -

Host bacterium

ID   1555 GenBank   NZ_JAGRZM010000011
Plasmid name   IncC Incompatibility group   IncA/C2
Plasmid size   120313 bp Coordinate of oriT [Strand]   52322..52472 [-]
Host baterium   Enterobacter hormaechei subsp. xiangfangensis strain PLCR20

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -