Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101108
Name   oriT_IncC in_silico
Organism   Enterobacter hormaechei subsp. xiangfangensis strain PLCR15
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAGQAU010000012 (76030..76180 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_IncC
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1127 GenBank   WP_001326173
Name   mobH_KBU53_RS16840_IncC insolico UniProt ID   F5BPR1
Length   990 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB F5BPR1


T4CP

ID   686 GenBank   WP_000178857
Name   traD_KBU53_RS16845_IncC insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69225.71 Da        Isoelectric Point: 6.9403

>WP_000178857.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKVKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-299)

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

  Protein structure



No available structure.



ID   687 GenBank   WP_000637384
Name   traC_KBU53_RS16925_IncC insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92604.86 Da        Isoelectric Point: 5.9216

>WP_000637384.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSQGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRRQAA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

TraG P-loop domain (P-loop_TraG)

(449-809)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 74114..99257

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBU53_RS16830 (KBU53_16800) 70145..70378 - 234 WP_001191890 hypothetical protein -
KBU53_RS16835 (KBU53_16805) 70360..70977 - 618 WP_001249395 hypothetical protein -
KBU53_RS16840 (KBU53_16810) 71145..74117 + 2973 WP_001326173 MobH family relaxase -
KBU53_RS16845 (KBU53_16815) 74114..75979 + 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
KBU53_RS16850 (KBU53_16820) 76029..76574 + 546 WP_000228720 hypothetical protein -
KBU53_RS16855 (KBU53_16825) 76531..77160 + 630 WP_000743449 DUF4400 domain-containing protein tfc7
KBU53_RS16860 (KBU53_16830) 77170..77616 + 447 WP_000122507 hypothetical protein -
KBU53_RS16865 (KBU53_16835) 77626..78003 + 378 WP_000869297 hypothetical protein -
KBU53_RS16870 (KBU53_16840) 78003..78665 + 663 WP_001231464 hypothetical protein -
KBU53_RS16875 (KBU53_16845) 78846..78989 + 144 WP_001275801 hypothetical protein -
KBU53_RS16880 (KBU53_16850) 79001..79366 + 366 WP_001052530 hypothetical protein -
KBU53_RS16885 (KBU53_16855) 79511..79792 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
KBU53_RS16890 (KBU53_16860) 79789..80415 + 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
KBU53_RS16895 (KBU53_16865) 80399..81316 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
KBU53_RS16900 (KBU53_16870) 81316..82629 + 1314 WP_024131605 TraB/VirB10 family protein traB
KBU53_RS16905 (KBU53_16875) 82626..83204 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
KBU53_RS16910 (KBU53_16880) 83208..83600 + 393 WP_000479535 TraA family conjugative transfer protein -
KBU53_RS16915 (KBU53_16885) 83801..89287 + 5487 WP_059453255 Ig-like domain-containing protein -
KBU53_RS16920 (KBU53_16890) 89436..90143 + 708 WP_001259346 DsbC family protein -
KBU53_RS16925 (KBU53_16895) 90140..92587 + 2448 WP_000637384 type IV secretion system protein TraC virb4
KBU53_RS16930 (KBU53_16900) 92602..92919 + 318 WP_000351984 hypothetical protein -
KBU53_RS16935 (KBU53_16905) 92916..93446 + 531 WP_001010740 S26 family signal peptidase -
KBU53_RS16940 (KBU53_16910) 93409..94674 + 1266 WP_001447719 TrbC family F-type conjugative pilus assembly protein traW
KBU53_RS16945 (KBU53_16915) 94671..95342 + 672 WP_000575345 EAL domain-containing protein -
KBU53_RS16950 (KBU53_16920) 95342..96346 + 1005 WP_005507569 TraU family protein traU
KBU53_RS16955 (KBU53_16925) 96462..99257 + 2796 WP_001256484 conjugal transfer mating pair stabilization protein TraN traN
KBU53_RS16960 (KBU53_16930) 99600..100460 - 861 WP_000709517 hypothetical protein -
KBU53_RS16965 (KBU53_16935) 100583..101224 - 642 WP_000796664 hypothetical protein -
KBU53_RS16970 (KBU53_16940) 101519..101839 + 321 WP_000547566 hypothetical protein -
KBU53_RS16975 (KBU53_16945) 102143..102328 + 186 WP_001186917 hypothetical protein -
KBU53_RS16980 (KBU53_16950) 102548..103516 + 969 WP_000085160 AAA family ATPase -

Host bacterium

ID   1552 GenBank   NZ_JAGQAU010000012
Plasmid name   IncC Incompatibility group   IncA/C2
Plasmid size   128230 bp Coordinate of oriT [Strand]   76030..76180 [+]
Host baterium   Enterobacter hormaechei subsp. xiangfangensis strain PLCR15

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -