Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101104
Name   oriT_p2_21222 in_silico
Organism   Escherichia coli strain 21222
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAKLTC010000003 (48203..48288 [+], 86 nt)
oriT length   86 nt
IRs (inverted repeats)      61..68, 73..80  (TTGGTGGT..ACCACCAA)
 27..34, 37..44  (GCAAAAAC..GTTTTTGC)
 8..14, 20..26  (TGATTTA..TAAATCA)
Location of nic site      53..54
Conserved sequence flanking the
  nic site    		  TGTGTGGTGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 86 nt

>oriT_p2_21222
AATTACATGATTTAAAACGTAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1125 GenBank   WP_000948354
Name   traI_L6501_RS23965_p2_21222 insolico UniProt ID   A0AA36L3I8
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191683.22 Da        Isoelectric Point: 5.6912

>WP_000948354.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLQEGMVFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISLQKTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVVIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGETFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREVARENLLQERLQQIERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1434-1557)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

TrwC relaxase (TrwC)

(10-283)

AAA domain (AAA_30)

(968-1156)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)


  Protein structure



No available structure.




Auxiliary protein

ID   405 GenBank   WP_000089263
Name   WP_000089263_p2_21222 insolico UniProt ID   A0A3U3XJL9
Length   75 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 75 a.a.        Molecular weight: 8541.74 Da        Isoelectric Point: 8.6796

>WP_000089263.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Gammaproteobacteria]
MSRNIIRPAPGNKVLLVLDDATNHKLLGARERSGRTKTNEVLVRLRDHLNRFPDFYNLDAIKEGAEETDS
IIKDL

  Protein domains


Predicted by InterproScan.

TraY domain (TraY)

(14-60)


  Protein structure


Source ID Structure
AlphaFold DB A0A3U3XJL9

ID   406 GenBank   WP_001354030
Name   WP_001354030_p2_21222 insolico UniProt ID   A0A734M5H3
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14508.47 Da        Isoelectric Point: 4.7116

>WP_001354030.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Gammaproteobacteria]
MARVILYISNDVYDKVNAIVEQRRQEGARDKDISVSGTASMLLELGLRVYEAQMERKESAFNQTEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPKNDEE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A734M5H3


T4CP

ID   683 GenBank   WP_000009330
Name   traD_L6501_RS23970_p2_21222 insolico UniProt ID   _
Length   744 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 744 a.a.        Molecular weight: 84607.33 Da        Isoelectric Point: 5.1644

>WP_000009330.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLVLWVKISWQTFINGCIYWWCTSLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGDPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQAEQPQQPQQPQQPQQ
PQQPQQPQQPQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESGEVV
DMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

  Protein structure



No available structure.



ID   684 GenBank   WP_000069777
Name   traC_L6501_RS24065_p2_21222 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 99195.86 Da        Isoelectric Point: 6.1126

>WP_000069777.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]
MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAI
PINGANKSIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAA
ATQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIV
GEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNPEIAF
LWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWG
ELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGK
GLFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSG
AGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLS
VMASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMIVLLDQY
TANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGW
RLLDFKNRKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKY
NQLYPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEG
LSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(290-447)

TraG P-loop domain (P-loop_TraG)

(468-764)

TraC protein (TraC_F_IV)

(39-277)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 20740..48856

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6501_RS23970 (L6501_23960) 20740..22974 - 2235 WP_000009330 type IV conjugative transfer system coupling protein TraD virb4
L6501_RS23975 (L6501_23965) 23025..23762 - 738 WP_000199905 hypothetical protein -
L6501_RS23980 (L6501_23970) 23965..24696 - 732 WP_000782451 conjugal transfer complement resistance protein TraT -
L6501_RS23985 (L6501_23975) 24745..25230 - 486 WP_000605870 hypothetical protein -
L6501_RS23990 (L6501_23980) 25246..28068 - 2823 WP_001007039 conjugal transfer mating-pair stabilization protein TraG traG
L6501_RS23995 (L6501_23985) 28065..29438 - 1374 WP_000944331 conjugal transfer pilus assembly protein TraH traH
L6501_RS24000 (L6501_23990) 29425..29817 - 393 WP_000660699 F-type conjugal transfer protein TrbF -
L6501_RS24005 (L6501_23995) 29798..30145 - 348 WP_001309242 P-type conjugative transfer protein TrbJ -
L6501_RS24010 (L6501_24000) 30075..30620 - 546 WP_000059831 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
L6501_RS24015 (L6501_24005) 30607..30891 - 285 WP_000624194 type-F conjugative transfer system pilin chaperone TraQ -
L6501_RS24020 (L6501_24010) 31010..31354 - 345 WP_000556796 conjugal transfer protein TrbA -
L6501_RS24025 (L6501_24015) 31368..32111 - 744 WP_001030371 type-F conjugative transfer system pilin assembly protein TraF traF
L6501_RS24030 (L6501_24020) 32104..32361 - 258 WP_000864353 conjugal transfer protein TrbE -
L6501_RS24035 (L6501_24025) 32388..34238 - 1851 WP_000821856 type-F conjugative transfer system mating-pair stabilization protein TraN traN
L6501_RS24040 (L6501_24030) 34235..34873 - 639 WP_001080256 type-F conjugative transfer system pilin assembly protein TrbC trbC
L6501_RS24045 (L6501_24035) 34882..35187 - 306 WP_000224416 hypothetical protein -
L6501_RS24050 (L6501_24040) 35217..36209 - 993 WP_000830838 conjugal transfer pilus assembly protein TraU traU
L6501_RS24055 (L6501_24045) 36206..36838 - 633 WP_001203728 type-F conjugative transfer system protein TraW traW
L6501_RS24060 (L6501_24050) 36835..37221 - 387 WP_000214084 type-F conjugative transfer system protein TrbI -
L6501_RS24065 (L6501_24055) 37218..39848 - 2631 WP_000069777 type IV secretion system protein TraC virb4
L6501_RS24070 (L6501_24060) 39974..40321 - 348 WP_000836682 hypothetical protein -
L6501_RS24075 (L6501_24065) 40349..40567 - 219 WP_000556745 hypothetical protein -
L6501_RS24080 (L6501_24070) 40647..41123 - 477 WP_000549587 hypothetical protein -
L6501_RS24085 (L6501_24075) 41116..41337 - 222 WP_001278683 conjugal transfer protein TraR -
L6501_RS24090 (L6501_24080) 41472..41987 - 516 WP_000809881 type IV conjugative transfer system lipoprotein TraV traV
L6501_RS24095 (L6501_24085) 41984..42304 - 321 WP_001057307 conjugal transfer protein TrbD -
L6501_RS24100 (L6501_24090) 42291..42857 - 567 WP_000896599 conjugal transfer pilus-stabilizing protein TraP -
L6501_RS24105 (L6501_24095) 42847..44298 - 1452 WP_000146675 F-type conjugal transfer pilus assembly protein TraB traB
L6501_RS24110 (L6501_24100) 44298..45026 - 729 WP_001230772 type-F conjugative transfer system secretin TraK traK
L6501_RS24115 (L6501_24105) 45013..45579 - 567 WP_000399780 type IV conjugative transfer system protein TraE traE
L6501_RS24120 (L6501_24110) 45601..45912 - 312 WP_000012113 type IV conjugative transfer system protein TraL traL
L6501_RS24125 (L6501_24115) 45927..46286 - 360 WP_001098992 type IV conjugative transfer system pilin TraA -
L6501_RS24130 (L6501_24120) 46319..46546 - 228 WP_000089263 conjugal transfer relaxosome protein TraY -
L6501_RS24135 (L6501_24125) 46683..47354 - 672 WP_000283561 conjugal transfer transcriptional regulator TraJ -
L6501_RS24140 (L6501_24130) 47548..47931 - 384 WP_001354030 conjugal transfer relaxosome DNA-binding protein TraM -
L6501_RS24145 (L6501_24135) 48254..48856 + 603 WP_000243713 transglycosylase SLT domain-containing protein -
L6501_RS24150 (L6501_24140) 49153..49974 - 822 WP_001234445 DUF932 domain-containing protein -
L6501_RS24155 (L6501_24145) 50085..50381 - 297 WP_001272251 hypothetical protein -
L6501_RS24160 (L6501_24150) 50681..50977 + 297 Protein_63 hypothetical protein -
L6501_RS24165 (L6501_24155) 51296..51421 - 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
L6501_RS24170 (L6501_24160) 51363..51512 - 150 Protein_65 plasmid maintenance protein Mok -
L6501_RS24175 (L6501_24165) 51534..51713 + 180 WP_001309233 hypothetical protein -
L6501_RS24180 (L6501_24170) 51691..52453 - 763 Protein_67 plasmid SOS inhibition protein A -
L6501_RS24185 (L6501_24175) 52450..52884 - 435 WP_000845953 conjugation system SOS inhibitor PsiB -

Host bacterium

ID   1548 GenBank   NZ_JAKLTC010000003
Plasmid name   p2_21222 Incompatibility group   IncFII
Plasmid size   98865 bp Coordinate of oriT [Strand]   48203..48288 [+]
Host baterium   Escherichia coli strain 21222

Cargo genes

Drug resistance gene   blaCTX-M-27, blaLAP-2, qnrS1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -