Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101101 |
| Name | oriT_pZJ3955  |
| Organism | Escherichia coli strain ZJ3955 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MCDN01000050 (43968..44020 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pZJ3955
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 680 | GenBank | WP_241227969 |
| Name | t4cp2_BEC86_RS19430_pZJ3955 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73403.08 Da Isoelectric Point: 9.5763
>WP_241227969.1 type IV secretory system conjugative DNA transfer family protein [Escherichia coli]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQKVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQKVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1687..25839
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BEC86_RS19380 (BEC86_24385) | 1..1035 | - | 1035 | WP_000750512 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| BEC86_RS19385 (BEC86_24390) | 1048..1683 | - | 636 | WP_069684560 | A24 family peptidase | - |
| BEC86_RS19390 (BEC86_24395) | 1687..2169 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| BEC86_RS19395 (BEC86_24400) | 2235..2792 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| BEC86_RS19400 (BEC86_24405) | 2837..3946 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| BEC86_RS19405 (BEC86_24410) | 3937..5475 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| BEC86_RS19410 (BEC86_24415) | 5500..5994 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| BEC86_RS19415 (BEC86_24420) | 5978..7288 | - | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| BEC86_RS19420 (BEC86_24425) | 7339..8982 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| BEC86_RS19425 (BEC86_24430) | 8975..9505 | - | 531 | WP_001220542 | sigma 54-interacting transcriptional regulator | virb4 |
| BEC86_RS19430 (BEC86_24435) | 9552..11510 | - | 1959 | WP_241227969 | type IV secretory system conjugative DNA transfer family protein | - |
| BEC86_RS19435 (BEC86_24440) | 11526..12581 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| BEC86_RS19440 (BEC86_24445) | 12600..13739 | - | 1140 | WP_000790640 | TrbI/VirB10 family protein | virB10 |
| BEC86_RS19445 (BEC86_24450) | 13729..14430 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| BEC86_RS19450 (BEC86_24455) | 14496..15230 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| BEC86_RS19455 (BEC86_24465) | 15396..17753 | - | 2358 | WP_063078682 | VirB4 family type IV secretion system protein | virb4 |
| BEC86_RS19460 (BEC86_24470) | 17759..18079 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| BEC86_RS19465 (BEC86_24475) | 18150..18440 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| BEC86_RS19470 (BEC86_24480) | 18440..19024 | - | 585 | WP_001177113 | lytic transglycosylase domain-containing protein | virB1 |
| BEC86_RS19475 (BEC86_24485) | 19045..19443 | - | 399 | WP_001153665 | hypothetical protein | - |
| BEC86_RS19480 (BEC86_24490) | 19562..19999 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| BEC86_RS19485 (BEC86_24495) | 20005..21240 | - | 1236 | WP_015059538 | TcpQ domain-containing protein | - |
| BEC86_RS19490 (BEC86_24500) | 21243..21542 | - | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| BEC86_RS19495 (BEC86_24505) | 21610..21891 | - | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| BEC86_RS19500 (BEC86_24510) | 21881..22132 | - | 252 | WP_000121741 | hypothetical protein | - |
| BEC86_RS19505 (BEC86_24515) | 22232..22867 | - | 636 | WP_015059536 | hypothetical protein | - |
| BEC86_RS19510 (BEC86_24520) | 22915..23706 | + | 792 | WP_023154636 | DUF5710 domain-containing protein | - |
| BEC86_RS19515 (BEC86_24525) | 23961..24185 | - | 225 | WP_000713561 | EexN family lipoprotein | - |
| BEC86_RS19520 (BEC86_24530) | 24194..24838 | - | 645 | WP_001310442 | type IV secretion system protein | - |
| BEC86_RS19525 (BEC86_24535) | 24844..25839 | - | 996 | WP_001028541 | type IV secretion system protein | virB6 |
| BEC86_RS19530 (BEC86_24540) | 25843..26100 | - | 258 | WP_000739144 | hypothetical protein | - |
| BEC86_RS19535 | 26097..26399 | - | 303 | WP_001360345 | hypothetical protein | - |
| BEC86_RS19540 (BEC86_24550) | 26670..26882 | - | 213 | WP_039022940 | hypothetical protein | - |
| BEC86_RS19545 | 26893..27063 | - | 171 | WP_000550720 | hypothetical protein | - |
| BEC86_RS19550 | 27067..27510 | - | 444 | WP_072037179 | NfeD family protein | - |
| BEC86_RS19555 (BEC86_24560) | 27884..28837 | - | 954 | WP_072109880 | SPFH domain-containing protein | - |
| BEC86_RS19560 | 28864..29040 | - | 177 | WP_000753050 | hypothetical protein | - |
| BEC86_RS19565 (BEC86_24565) | 29033..29248 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| BEC86_RS19570 (BEC86_24570) | 29241..29693 | - | 453 | WP_223666486 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 1545 | GenBank | NZ_MCDN01000050 |
| Plasmid name | pZJ3955 | Incompatibility group | IncI2 |
| Plasmid size | 59576 bp | Coordinate of oriT [Strand] | 43968..44020 [-] |
| Host baterium | Escherichia coli strain ZJ3955 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |