Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101098 |
| Name | oriT_pZJ292  |
| Organism | Escherichia coli strain ZJ292 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MCDG01000074 (15549..15601 [+], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pZJ292
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 679 | GenBank | WP_015059539 |
| Name | t4cp2_BEC79_RS21520_pZJ292 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 33964..57415
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BEC79_RS21375 (BEC79_23470) | 29876..30328 | + | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
| BEC79_RS21380 (BEC79_23475) | 30321..30536 | + | 216 | WP_001127357 | DUF1187 family protein | - |
| BEC79_RS21385 | 30529..30705 | + | 177 | WP_000753050 | hypothetical protein | - |
| BEC79_RS21390 (BEC79_23480) | 30732..31685 | + | 954 | WP_072089442 | SPFH domain-containing protein | - |
| BEC79_RS21395 | 32059..32502 | + | 444 | WP_000964330 | NfeD family protein | - |
| BEC79_RS21400 | 32506..32676 | + | 171 | WP_000550720 | hypothetical protein | - |
| BEC79_RS21405 (BEC79_23490) | 32687..33133 | + | 447 | WP_001243165 | hypothetical protein | - |
| BEC79_RS21410 (BEC79_23495) | 33166..33423 | + | 258 | WP_001542015 | hypothetical protein | - |
| BEC79_RS21415 | 33404..33706 | + | 303 | WP_001360345 | hypothetical protein | - |
| BEC79_RS21420 (BEC79_23500) | 33703..33960 | + | 258 | WP_000739144 | hypothetical protein | - |
| BEC79_RS21425 (BEC79_23505) | 33964..34959 | + | 996 | WP_001028543 | type IV secretion system protein | virB6 |
| BEC79_RS21430 (BEC79_23510) | 34965..35606 | + | 642 | WP_001425343 | type IV secretion system protein | - |
| BEC79_RS21435 (BEC79_23515) | 35608..35862 | + | 255 | WP_001043555 | EexN family lipoprotein | - |
| BEC79_RS21440 (BEC79_23520) | 35875..36162 | + | 288 | WP_001032611 | EexN family lipoprotein | - |
| BEC79_RS21445 (BEC79_23525) | 36235..36870 | + | 636 | WP_015059536 | hypothetical protein | - |
| BEC79_RS21450 (BEC79_23530) | 36970..37221 | + | 252 | WP_000121741 | hypothetical protein | - |
| BEC79_RS21455 (BEC79_23535) | 37211..37492 | + | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| BEC79_RS21460 (BEC79_23540) | 37560..37859 | + | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| BEC79_RS21465 (BEC79_23545) | 37862..39097 | + | 1236 | WP_015059538 | TcpQ domain-containing protein | - |
| BEC79_RS21470 (BEC79_23550) | 39103..39540 | + | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| BEC79_RS21475 (BEC79_23555) | 39659..40057 | + | 399 | WP_072643816 | hypothetical protein | - |
| BEC79_RS21480 (BEC79_23560) | 40078..40662 | + | 585 | WP_001177113 | lytic transglycosylase domain-containing protein | virB1 |
| BEC79_RS21485 (BEC79_23565) | 40662..40952 | + | 291 | WP_000865479 | conjugal transfer protein | - |
| BEC79_RS21490 (BEC79_23570) | 41023..41343 | + | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| BEC79_RS21495 (BEC79_23575) | 41349..43706 | + | 2358 | WP_063078682 | VirB4 family type IV secretion system protein | virb4 |
| BEC79_RS21500 (BEC79_23585) | 43872..44606 | + | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| BEC79_RS21505 (BEC79_23590) | 44672..45373 | + | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| BEC79_RS21510 (BEC79_23595) | 45363..46502 | + | 1140 | WP_000790640 | TrbI/VirB10 family protein | virB10 |
| BEC79_RS21515 (BEC79_23600) | 46521..47576 | + | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| BEC79_RS21520 (BEC79_23605) | 47592..49550 | + | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| BEC79_RS21525 (BEC79_23610) | 49597..50127 | + | 531 | WP_001220542 | sigma 54-interacting transcriptional regulator | virb4 |
| BEC79_RS21530 (BEC79_23615) | 50120..51763 | + | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| BEC79_RS21535 (BEC79_23620) | 51814..53124 | + | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| BEC79_RS21540 (BEC79_23625) | 53108..53602 | + | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| BEC79_RS21545 (BEC79_23630) | 53627..55165 | + | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| BEC79_RS21550 (BEC79_23635) | 55156..56265 | + | 1110 | WP_000974903 | type II secretion system F family protein | - |
| BEC79_RS21555 (BEC79_23640) | 56310..56867 | + | 558 | WP_000095048 | type 4 pilus major pilin | - |
| BEC79_RS21560 (BEC79_23645) | 56933..57415 | + | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| BEC79_RS21565 (BEC79_23650) | 57419..58054 | + | 636 | WP_000934977 | A24 family peptidase | - |
| BEC79_RS21570 (BEC79_23655) | 58067..59101 | + | 1035 | WP_000750512 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
Host bacterium
| ID | 1542 | GenBank | NZ_MCDG01000074 |
| Plasmid name | pZJ292 | Incompatibility group | IncI2 |
| Plasmid size | 59101 bp | Coordinate of oriT [Strand] | 15549..15601 [+] |
| Host baterium | Escherichia coli strain ZJ292 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |