Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101095 |
| Name | oriT_pZJ1364  |
| Organism | Escherichia coli strain ZJ1364 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MCDL01000098 (14890..14942 [+], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pZJ1364
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 678 | GenBank | WP_015059539 |
| Name | t4cp2_BEC84_RS22275_pZJ1364 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 33305..56762
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BEC84_RS22130 (BEC84_05620) | 29217..29669 | + | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
| BEC84_RS22135 (BEC84_05625) | 29662..29877 | + | 216 | WP_001127357 | DUF1187 family protein | - |
| BEC84_RS22140 | 29870..30046 | + | 177 | WP_000753050 | hypothetical protein | - |
| BEC84_RS22145 (BEC84_05630) | 30073..31026 | + | 954 | WP_072089442 | SPFH domain-containing protein | - |
| BEC84_RS22150 | 31400..31843 | + | 444 | WP_000964330 | NfeD family protein | - |
| BEC84_RS22155 | 31847..32017 | + | 171 | WP_000550720 | hypothetical protein | - |
| BEC84_RS22160 (BEC84_05640) | 32028..32474 | + | 447 | WP_001243165 | hypothetical protein | - |
| BEC84_RS22165 (BEC84_05645) | 32507..32764 | + | 258 | WP_001542015 | hypothetical protein | - |
| BEC84_RS22170 | 32745..33047 | + | 303 | WP_001360345 | hypothetical protein | - |
| BEC84_RS22175 (BEC84_05650) | 33044..33301 | + | 258 | WP_000739144 | hypothetical protein | - |
| BEC84_RS22180 (BEC84_05655) | 33305..34300 | + | 996 | WP_001028543 | type IV secretion system protein | virB6 |
| BEC84_RS22185 (BEC84_05660) | 34306..34947 | + | 642 | WP_001425343 | type IV secretion system protein | - |
| BEC84_RS22190 (BEC84_05665) | 34949..35203 | + | 255 | WP_001043555 | EexN family lipoprotein | - |
| BEC84_RS22195 (BEC84_05670) | 35216..35503 | + | 288 | WP_001032611 | EexN family lipoprotein | - |
| BEC84_RS22200 (BEC84_05675) | 35576..36211 | + | 636 | WP_241225885 | hypothetical protein | - |
| BEC84_RS22205 (BEC84_05680) | 36311..36562 | + | 252 | WP_000121741 | hypothetical protein | - |
| BEC84_RS22210 (BEC84_05685) | 36552..36833 | + | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| BEC84_RS22215 (BEC84_05690) | 36901..37200 | + | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| BEC84_RS22220 (BEC84_05695) | 37203..38438 | + | 1236 | WP_015059538 | TcpQ domain-containing protein | - |
| BEC84_RS22225 (BEC84_05700) | 38444..38881 | + | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| BEC84_RS22230 (BEC84_05705) | 39000..39398 | + | 399 | WP_001153665 | hypothetical protein | - |
| BEC84_RS22235 (BEC84_05710) | 39419..40003 | + | 585 | WP_001177113 | lytic transglycosylase domain-containing protein | virB1 |
| BEC84_RS22240 (BEC84_05715) | 40003..40293 | + | 291 | WP_000865479 | conjugal transfer protein | - |
| BEC84_RS22245 (BEC84_05720) | 40364..40684 | + | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| BEC84_RS22250 (BEC84_05725) | 40690..43047 | + | 2358 | WP_063078682 | VirB4 family type IV secretion system protein | virb4 |
| BEC84_RS22255 (BEC84_05735) | 43213..43947 | + | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| BEC84_RS22260 (BEC84_05740) | 44013..44714 | + | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| BEC84_RS22265 (BEC84_05745) | 44704..45843 | + | 1140 | WP_000790640 | TrbI/VirB10 family protein | virB10 |
| BEC84_RS22270 (BEC84_05750) | 45862..46917 | + | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| BEC84_RS22275 (BEC84_05755) | 46933..48891 | + | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| BEC84_RS22280 (BEC84_05760) | 48938..49474 | + | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| BEC84_RS22285 (BEC84_05765) | 49467..51110 | + | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| BEC84_RS22290 (BEC84_05770) | 51161..52471 | + | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| BEC84_RS22295 (BEC84_05775) | 52455..52949 | + | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| BEC84_RS22300 (BEC84_05780) | 52974..54512 | + | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| BEC84_RS22305 (BEC84_05785) | 54503..55612 | + | 1110 | WP_000974903 | type II secretion system F family protein | - |
| BEC84_RS22310 (BEC84_05790) | 55657..56214 | + | 558 | WP_000095048 | type 4 pilus major pilin | - |
| BEC84_RS22315 (BEC84_05795) | 56280..56762 | + | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| BEC84_RS22320 (BEC84_05800) | 56766..57401 | + | 636 | WP_000934977 | A24 family peptidase | - |
| BEC84_RS22325 (BEC84_05805) | 57414..58448 | + | 1035 | WP_000750512 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
Host bacterium
| ID | 1539 | GenBank | NZ_MCDL01000098 |
| Plasmid name | pZJ1364 | Incompatibility group | IncI2 |
| Plasmid size | 58448 bp | Coordinate of oriT [Strand] | 14890..14942 [+] |
| Host baterium | Escherichia coli strain ZJ1364 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |