Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101094 |
| Name | oriT_pZJ623  |
| Organism | Escherichia coli strain ZJ623 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MCFY01000007 (44181..44283 [+], 103 nt) |
| oriT length | 103 nt |
| IRs (inverted repeats) | 64..70, 73..79 (CCACCGT..ACGGTGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 103 nt
>oriT_pZJ623
AGCCTCGCAGAGCAGGATTCCCGTTGAGCAGCCCAGCTGCGAATAAGGGAAGGTAAAGTTGGACCACCGTTTACGGTGGGCCTACTTTACATATCCTGCCCGG
AGCCTCGCAGAGCAGGATTCCCGTTGAGCAGCCCAGCTGCGAATAAGGGAAGGTAAAGTTGGACCACCGTTTACGGTGGGCCTACTTTACATATCCTGCCCGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1123 | GenBank | WP_050580356 |
| Name | Relaxase_BEC63_RS01085_pZJ623 |
UniProt ID | _ |
| Length | 869 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 869 a.a. Molecular weight: 96507.42 Da Isoelectric Point: 10.4435
>WP_050580356.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MKNIKKSSFSGLADYITNPQSKQERVGVIKATNCQSQDVQDAVTEVNITQRLNTRSESDKTYHLVISFRP
GEKPSEDVLNAIEESICQSLGFGDHQRLSAVHHDTDNLHIHVAINKINPDTLTIHNPYNDYRVLAESCAY
LEKKYGLEVDNHMPTRSASENRAKDMERHSGIQSLLSWVKAECAEQLLNANSWQELHQAMRQNGLELRQK
GNGFVIAGPDGLAVKASSVDRSLSRAALEKKLGSFESSIPDTKPSRPSPEAIKQRFGSAANVKRPGDRPP
PMRENRQVHLGDVPVMQINSGKRYESKPLGSQGANTTELYGRYQSEQQRLTAARNEALRLAKVKRDRLIE
NAKRSGRLKRATIKLLKGPGVNKRYLYGLASKALGEELERANAIYKKDRQAAYDIHKRRTWADWLQFKAA
GGDDEALKTLRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDGGKRLDVSR
DAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFADPELEKLRQILTKKAAREREYI
HIPFEKRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIRMNEEKDNEQRRQRYAGTERTDAGRERSGNG
NATGANEPGVTGRSTSADAGNGYGTSSSRRRTGTGDAVTTAGSQPGRSDDTRTGRSRAAEPNGERGHSKP
NVARPGRQPPPTAKNRLRAMSELGMVRIAGGSEVLLQGNVSDQLEHKTAERADQLRRDMAGAGGITTPAE
VKKPLSSAEKYINEREQKRLKGFDIPKHRGYNSTDKGLFEYAGQRKSGLETLALLKRDDEIVVMPVSDYT
ARRLSNLKVGESVTLTPSGSVRTTKGRSR
MKNIKKSSFSGLADYITNPQSKQERVGVIKATNCQSQDVQDAVTEVNITQRLNTRSESDKTYHLVISFRP
GEKPSEDVLNAIEESICQSLGFGDHQRLSAVHHDTDNLHIHVAINKINPDTLTIHNPYNDYRVLAESCAY
LEKKYGLEVDNHMPTRSASENRAKDMERHSGIQSLLSWVKAECAEQLLNANSWQELHQAMRQNGLELRQK
GNGFVIAGPDGLAVKASSVDRSLSRAALEKKLGSFESSIPDTKPSRPSPEAIKQRFGSAANVKRPGDRPP
PMRENRQVHLGDVPVMQINSGKRYESKPLGSQGANTTELYGRYQSEQQRLTAARNEALRLAKVKRDRLIE
NAKRSGRLKRATIKLLKGPGVNKRYLYGLASKALGEELERANAIYKKDRQAAYDIHKRRTWADWLQFKAA
GGDDEALKTLRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDGGKRLDVSR
DAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFADPELEKLRQILTKKAAREREYI
HIPFEKRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIRMNEEKDNEQRRQRYAGTERTDAGRERSGNG
NATGANEPGVTGRSTSADAGNGYGTSSSRRRTGTGDAVTTAGSQPGRSDDTRTGRSRAAEPNGERGHSKP
NVARPGRQPPPTAKNRLRAMSELGMVRIAGGSEVLLQGNVSDQLEHKTAERADQLRRDMAGAGGITTPAE
VKKPLSSAEKYINEREQKRLKGFDIPKHRGYNSTDKGLFEYAGQRKSGLETLALLKRDDEIVVMPVSDYT
ARRLSNLKVGESVTLTPSGSVRTTKGRSR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 677 | GenBank | WP_032310210 |
| Name | t4cp2_BEC63_RS01075_pZJ623 |
UniProt ID | _ |
| Length | 648 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 648 a.a. Molecular weight: 71711.82 Da Isoelectric Point: 8.3842
>WP_032310210.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MRKPNNAVGPQVRNKKKTARGKLIPAMAITSLAAGIQAATQYFAYSFNYQELLGPNFNHIYAPWSYFKWY
SAWHDRLPDAFFAAGSVGATVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIKAAGLLGNDEGV
YVGAWEDKNGQLQYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKEYA
KNLCLKFEPAAANGSVAWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVL
YKHKNDGTPATLPYVDSIMADPERGAGELWMEMTQYGHVNGENHPVVGAAGRDMMDRPEEEAGSVLSTAK
SYLALFRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERV
NNNLTLWQRFKQAFGFSVQGTKRVQTKKTYKHRLLAMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQD
INQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAILGGVSKTM
QEVQRPLLTVDECLRMPGPKKNAEGLITERGDMVVYVAGFPAIYGKQPLFFQDEIFSLRASVPEPKTTDR
IRAAVAANDDASNEAIAI
MRKPNNAVGPQVRNKKKTARGKLIPAMAITSLAAGIQAATQYFAYSFNYQELLGPNFNHIYAPWSYFKWY
SAWHDRLPDAFFAAGSVGATVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIKAAGLLGNDEGV
YVGAWEDKNGQLQYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKEYA
KNLCLKFEPAAANGSVAWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVL
YKHKNDGTPATLPYVDSIMADPERGAGELWMEMTQYGHVNGENHPVVGAAGRDMMDRPEEEAGSVLSTAK
SYLALFRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERV
NNNLTLWQRFKQAFGFSVQGTKRVQTKKTYKHRLLAMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQD
INQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAILGGVSKTM
QEVQRPLLTVDECLRMPGPKKNAEGLITERGDMVVYVAGFPAIYGKQPLFFQDEIFSLRASVPEPKTTDR
IRAAVAANDDASNEAIAI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 10425..20743
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BEC63_RS00870 (BEC63_21395) | 5988..6185 | - | 198 | WP_048207659 | DUF905 family protein | - |
| BEC63_RS00875 | 6646..6858 | - | 213 | WP_223307920 | hypothetical protein | - |
| BEC63_RS00880 (BEC63_21405) | 6855..7103 | - | 249 | WP_071443967 | antirestriction protein ArdR | - |
| BEC63_RS00885 (BEC63_21410) | 7103..7411 | - | 309 | WP_003019666 | HigA family addiction module antitoxin | - |
| BEC63_RS00890 (BEC63_21415) | 7420..7719 | - | 300 | WP_071443968 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| BEC63_RS00895 (BEC63_21420) | 8568..9446 | - | 879 | WP_048207660 | plasmid replication initiator TrfA | - |
| BEC63_RS23980 | 9584..9763 | - | 180 | WP_306433452 | single-stranded DNA-binding protein | - |
| BEC63_RS00900 (BEC63_21425) | 9836..10240 | + | 405 | WP_048207662 | transcriptional regulator | - |
| BEC63_RS00905 (BEC63_21430) | 10425..11381 | + | 957 | WP_032932562 | P-type conjugative transfer ATPase TrbB | virB11 |
| BEC63_RS00910 (BEC63_21435) | 11495..11878 | + | 384 | WP_003019650 | TrbC/VirB2 family protein | virB2 |
| BEC63_RS00915 (BEC63_21440) | 11881..12207 | + | 327 | WP_003019648 | conjugal transfer protein TrbD | virB3 |
| BEC63_RS00920 (BEC63_21445) | 12204..14747 | + | 2544 | WP_071443970 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| BEC63_RS00925 (BEC63_21450) | 14744..15451 | + | 708 | WP_048207665 | VirB8/TrbF family protein | virB8 |
| BEC63_RS00930 (BEC63_21455) | 15467..16357 | + | 891 | WP_003019641 | P-type conjugative transfer protein TrbG | virB9 |
| BEC63_RS00935 (BEC63_21460) | 16372..16812 | + | 441 | WP_024252959 | conjugal transfer protein TrbH | - |
| BEC63_RS00940 (BEC63_21465) | 16816..18210 | + | 1395 | WP_048207667 | TrbI/VirB10 family protein | virB10 |
| BEC63_RS00945 (BEC63_21470) | 18223..18993 | + | 771 | WP_046960647 | P-type conjugative transfer protein TrbJ | virB5 |
| BEC63_RS00950 (BEC63_21475) | 19006..19179 | + | 174 | WP_024252956 | entry exclusion lipoprotein TrbK | - |
| BEC63_RS00955 (BEC63_21480) | 19184..20743 | + | 1560 | WP_048207669 | P-type conjugative transfer protein TrbL | virB6 |
| BEC63_RS00960 (BEC63_21485) | 20754..21059 | + | 306 | WP_032310194 | lipoprotein | - |
| BEC63_RS00965 (BEC63_21490) | 21075..21845 | + | 771 | WP_048207671 | conjugal transfer protein | - |
| BEC63_RS00970 (BEC63_21495) | 21862..22548 | + | 687 | WP_053065212 | TraX family protein | - |
| BEC63_RS00975 (BEC63_21505) | 23133..23900 | - | 768 | WP_241220283 | PAP2 family protein | - |
| BEC63_RS00980 (BEC63_21510) | 23972..25597 | - | 1626 | WP_049589868 | phosphoethanolamine--lipid A transferase MCR-1.1 | - |
Host bacterium
| ID | 1538 | GenBank | NZ_MCFY01000007 |
| Plasmid name | pZJ623 | Incompatibility group | IncP1 |
| Plasmid size | 47409 bp | Coordinate of oriT [Strand] | 44181..44283 [+] |
| Host baterium | Escherichia coli strain ZJ623 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |