Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101093 |
| Name | oriT_pZJ296  |
| Organism | Escherichia coli strain ZJ296 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MCDJ01000057 (43501..43553 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pZJ296
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 676 | GenBank | WP_015059539 |
| Name | t4cp2_BEC82_RS16620_pZJ296 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1687..25138
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BEC82_RS16570 (BEC82_20985) | 1..1035 | - | 1035 | WP_000750512 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| BEC82_RS16575 (BEC82_20990) | 1048..1683 | - | 636 | WP_000934977 | A24 family peptidase | - |
| BEC82_RS16580 (BEC82_20995) | 1687..2169 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| BEC82_RS16585 (BEC82_21000) | 2235..2792 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| BEC82_RS16590 (BEC82_21005) | 2837..3946 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| BEC82_RS16595 (BEC82_21010) | 3937..5475 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| BEC82_RS16600 (BEC82_21015) | 5500..5994 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| BEC82_RS16605 (BEC82_21020) | 5978..7300 | - | 1323 | WP_000454142 | type 4b pilus protein PilO2 | - |
| BEC82_RS16610 (BEC82_21025) | 7339..8982 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| BEC82_RS16615 (BEC82_21030) | 8975..9505 | - | 531 | WP_001220542 | sigma 54-interacting transcriptional regulator | virb4 |
| BEC82_RS16620 (BEC82_21035) | 9552..11510 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| BEC82_RS16625 (BEC82_21040) | 11526..12581 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| BEC82_RS16630 (BEC82_21045) | 12600..13739 | - | 1140 | WP_000790640 | TrbI/VirB10 family protein | virB10 |
| BEC82_RS16635 (BEC82_21050) | 13729..14430 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| BEC82_RS16640 (BEC82_21055) | 14496..15230 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| BEC82_RS16645 (BEC82_21065) | 15396..17753 | - | 2358 | WP_063078682 | VirB4 family type IV secretion system protein | virb4 |
| BEC82_RS16650 (BEC82_21070) | 17759..18079 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| BEC82_RS16655 (BEC82_21075) | 18150..18440 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| BEC82_RS16660 (BEC82_21080) | 18440..19024 | - | 585 | WP_001177113 | lytic transglycosylase domain-containing protein | virB1 |
| BEC82_RS16665 (BEC82_21085) | 19045..19443 | - | 399 | WP_072643816 | hypothetical protein | - |
| BEC82_RS16670 (BEC82_21090) | 19562..19999 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| BEC82_RS16675 (BEC82_21095) | 20005..21240 | - | 1236 | WP_015059538 | TcpQ domain-containing protein | - |
| BEC82_RS16680 (BEC82_21100) | 21243..21542 | - | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| BEC82_RS16685 (BEC82_21105) | 21610..21891 | - | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| BEC82_RS16690 (BEC82_21110) | 21881..22132 | - | 252 | WP_000121741 | hypothetical protein | - |
| BEC82_RS16695 (BEC82_21115) | 22232..22867 | - | 636 | WP_015059536 | hypothetical protein | - |
| BEC82_RS16700 (BEC82_21120) | 22940..23227 | - | 288 | WP_001032611 | EexN family lipoprotein | - |
| BEC82_RS16705 (BEC82_21125) | 23240..23494 | - | 255 | WP_001043555 | EexN family lipoprotein | - |
| BEC82_RS16710 (BEC82_21130) | 23496..24137 | - | 642 | WP_001425343 | type IV secretion system protein | - |
| BEC82_RS16715 (BEC82_21135) | 24143..25138 | - | 996 | WP_001028543 | type IV secretion system protein | virB6 |
| BEC82_RS16720 (BEC82_21140) | 25142..25399 | - | 258 | WP_000739144 | hypothetical protein | - |
| BEC82_RS16725 | 25396..25698 | - | 303 | WP_001360345 | hypothetical protein | - |
| BEC82_RS16730 (BEC82_21145) | 25679..25936 | - | 258 | WP_001542015 | hypothetical protein | - |
| BEC82_RS16735 (BEC82_21150) | 25969..26415 | - | 447 | WP_001243165 | hypothetical protein | - |
| BEC82_RS16740 | 26426..26596 | - | 171 | WP_000550720 | hypothetical protein | - |
| BEC82_RS16745 | 26600..27043 | - | 444 | WP_000964330 | NfeD family protein | - |
| BEC82_RS16750 (BEC82_21160) | 27417..28370 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| BEC82_RS16755 | 28397..28573 | - | 177 | WP_000753050 | hypothetical protein | - |
| BEC82_RS16760 (BEC82_21165) | 28566..28781 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| BEC82_RS16765 (BEC82_21170) | 28774..29226 | - | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 1537 | GenBank | NZ_MCDJ01000057 |
| Plasmid name | pZJ296 | Incompatibility group | IncI2 |
| Plasmid size | 59211 bp | Coordinate of oriT [Strand] | 43501..43553 [-] |
| Host baterium | Escherichia coli strain ZJ296 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |