Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101092 |
| Name | oriT_pZJ1336  |
| Organism | Escherichia coli strain ZJ1336 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MCCO01000044 (14924..14976 [+], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pZJ1336
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 675 | GenBank | WP_219014804 |
| Name | t4cp2_BEC59_RS19925_pZJ1336 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73434.05 Da Isoelectric Point: 9.4339
>WP_219014804.1 type IV secretory system conjugative DNA transfer family protein [Escherichia coli]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VTGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VTGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 33709..57874
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BEC59_RS19780 (BEC59_25705) | 29855..30307 | + | 453 | WP_223666486 | CaiF/GrlA family transcriptional regulator | - |
| BEC59_RS19785 (BEC59_25710) | 30300..30515 | + | 216 | WP_001127357 | DUF1187 family protein | - |
| BEC59_RS19790 | 30508..30684 | + | 177 | WP_000753050 | hypothetical protein | - |
| BEC59_RS19795 (BEC59_25715) | 30711..31664 | + | 954 | WP_072109880 | SPFH domain-containing protein | - |
| BEC59_RS19800 | 32038..32481 | + | 444 | WP_072037179 | NfeD family protein | - |
| BEC59_RS19805 | 32485..32655 | + | 171 | WP_000550720 | hypothetical protein | - |
| BEC59_RS19810 (BEC59_25725) | 32666..32878 | + | 213 | WP_039022940 | hypothetical protein | - |
| BEC59_RS19815 | 33149..33451 | + | 303 | WP_001360345 | hypothetical protein | - |
| BEC59_RS19820 (BEC59_25735) | 33448..33705 | + | 258 | WP_000739144 | hypothetical protein | - |
| BEC59_RS19825 (BEC59_25740) | 33709..34704 | + | 996 | WP_001028541 | type IV secretion system protein | virB6 |
| BEC59_RS19830 (BEC59_25745) | 34710..35354 | + | 645 | WP_001310442 | type IV secretion system protein | - |
| BEC59_RS19835 (BEC59_25750) | 35363..35587 | + | 225 | WP_000713561 | EexN family lipoprotein | - |
| BEC59_RS19840 (BEC59_25755) | 35842..36633 | - | 792 | WP_023154636 | DUF5710 domain-containing protein | - |
| BEC59_RS19845 (BEC59_25760) | 36681..37316 | + | 636 | WP_015059536 | hypothetical protein | - |
| BEC59_RS19850 (BEC59_25765) | 37416..37667 | + | 252 | WP_000121741 | hypothetical protein | - |
| BEC59_RS19855 (BEC59_25770) | 37657..37938 | + | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| BEC59_RS19860 (BEC59_25775) | 38006..38305 | + | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| BEC59_RS19865 (BEC59_25780) | 38308..39543 | + | 1236 | WP_015059538 | TcpQ domain-containing protein | - |
| BEC59_RS19870 (BEC59_25785) | 39549..39986 | + | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| BEC59_RS19875 (BEC59_25790) | 40105..40503 | + | 399 | WP_001153665 | hypothetical protein | - |
| BEC59_RS19880 (BEC59_25795) | 40524..41108 | + | 585 | WP_219014805 | lytic transglycosylase domain-containing protein | virB1 |
| BEC59_RS19885 (BEC59_25800) | 41108..41398 | + | 291 | WP_000865479 | conjugal transfer protein | - |
| BEC59_RS19890 (BEC59_25805) | 41469..41789 | + | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| BEC59_RS19895 (BEC59_25810) | 41795..44152 | + | 2358 | WP_063078682 | VirB4 family type IV secretion system protein | virb4 |
| BEC59_RS19900 (BEC59_25815) | 44184..44318 | + | 135 | WP_000701233 | hypothetical protein | - |
| BEC59_RS19905 (BEC59_25820) | 44318..45052 | + | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| BEC59_RS19910 (BEC59_25825) | 45118..45819 | + | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| BEC59_RS19915 (BEC59_25830) | 45809..46948 | + | 1140 | WP_000790640 | TrbI/VirB10 family protein | virB10 |
| BEC59_RS19920 (BEC59_25835) | 46967..48022 | + | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| BEC59_RS19925 (BEC59_25840) | 48038..49996 | + | 1959 | WP_219014804 | type IV secretory system conjugative DNA transfer family protein | - |
| BEC59_RS19930 (BEC59_25845) | 50043..50585 | + | 543 | WP_001220544 | sigma 54-interacting transcriptional regulator | virb4 |
| BEC59_RS19935 (BEC59_25850) | 50578..52221 | + | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| BEC59_RS19940 (BEC59_25855) | 52272..53582 | + | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| BEC59_RS19945 (BEC59_25860) | 53566..54060 | + | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| BEC59_RS19950 (BEC59_25865) | 54085..55623 | + | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| BEC59_RS19955 (BEC59_25870) | 55614..56723 | + | 1110 | WP_000974903 | type II secretion system F family protein | - |
| BEC59_RS19960 (BEC59_25875) | 56768..57325 | + | 558 | WP_000095049 | type 4 pilus major pilin | - |
| BEC59_RS19965 (BEC59_25880) | 57392..57874 | + | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| BEC59_RS19970 (BEC59_25885) | 57878..58513 | + | 636 | WP_000934978 | A24 family peptidase | - |
| BEC59_RS19975 (BEC59_25890) | 58526..59560 | + | 1035 | WP_000750512 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
Host bacterium
| ID | 1536 | GenBank | NZ_MCCO01000044 |
| Plasmid name | pZJ1336 | Incompatibility group | IncI2 |
| Plasmid size | 59560 bp | Coordinate of oriT [Strand] | 14924..14976 [+] |
| Host baterium | Escherichia coli strain ZJ1336 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |