Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101091 |
| Name | oriT_pZJ807  |
| Organism | Escherichia coli strain ZJ807 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MCCT01000063 (15501..15553 [+], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pZJ807
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 674 | GenBank | WP_015059539 |
| Name | t4cp2_BEC65_RS21805_pZJ807 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 36191..59021
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BEC65_RS21675 (BEC65_24960) | 32103..32555 | + | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
| BEC65_RS21680 (BEC65_24965) | 32548..32763 | + | 216 | WP_001127357 | DUF1187 family protein | - |
| BEC65_RS21685 | 32756..32932 | + | 177 | WP_000753050 | hypothetical protein | - |
| BEC65_RS21690 (BEC65_24970) | 32959..33912 | + | 954 | WP_072089442 | SPFH domain-containing protein | - |
| BEC65_RS21695 | 34286..34729 | + | 444 | WP_000964330 | NfeD family protein | - |
| BEC65_RS21700 | 34733..34903 | + | 171 | WP_000550720 | hypothetical protein | - |
| BEC65_RS21705 (BEC65_24980) | 34914..35360 | + | 447 | WP_001243165 | hypothetical protein | - |
| BEC65_RS21710 (BEC65_24985) | 35393..35650 | + | 258 | WP_001542015 | hypothetical protein | - |
| BEC65_RS21715 | 35631..35933 | + | 303 | WP_001360345 | hypothetical protein | - |
| BEC65_RS21720 (BEC65_24990) | 35930..36187 | + | 258 | WP_000739144 | hypothetical protein | - |
| BEC65_RS21725 (BEC65_24995) | 36191..37186 | + | 996 | WP_001028540 | type IV secretion system protein | virB6 |
| BEC65_RS21730 (BEC65_25000) | 37192..37836 | + | 645 | WP_001310442 | type IV secretion system protein | - |
| BEC65_RS21735 (BEC65_25005) | 37845..38069 | + | 225 | WP_000713562 | EexN family lipoprotein | - |
| BEC65_RS21740 (BEC65_25010) | 38292..39101 | - | 810 | WP_024237698 | DUF5710 domain-containing protein | - |
| BEC65_RS21745 (BEC65_25015) | 39149..39448 | + | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| BEC65_RS21750 (BEC65_25020) | 39451..40686 | + | 1236 | WP_034169417 | toxin co-regulated pilus biosynthesis Q family protein | - |
| BEC65_RS21755 (BEC65_25025) | 40692..41129 | + | 438 | WP_034169416 | type IV pilus biogenesis protein PilM | - |
| BEC65_RS21760 (BEC65_25030) | 41248..41646 | + | 399 | WP_001153669 | hypothetical protein | - |
| BEC65_RS21765 (BEC65_25035) | 41667..42251 | + | 585 | WP_001177117 | lytic transglycosylase domain-containing protein | virB1 |
| BEC65_RS21770 (BEC65_25040) | 42251..42541 | + | 291 | WP_000865479 | conjugal transfer protein | - |
| BEC65_RS21775 (BEC65_25045) | 42612..42932 | + | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| BEC65_RS21780 (BEC65_25050) | 42938..45295 | + | 2358 | WP_000548950 | VirB4 family type IV secretion system protein | virb4 |
| BEC65_RS21785 (BEC65_25060) | 45461..46195 | + | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| BEC65_RS21790 (BEC65_25065) | 46261..46962 | + | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| BEC65_RS21795 (BEC65_25070) | 46952..48091 | + | 1140 | WP_034169415 | TrbI/VirB10 family protein | virB10 |
| BEC65_RS21800 (BEC65_25075) | 48110..49165 | + | 1056 | WP_001542006 | P-type DNA transfer ATPase VirB11 | virB11 |
| BEC65_RS21805 (BEC65_25080) | 49181..51139 | + | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| BEC65_RS21810 (BEC65_25085) | 51186..51722 | + | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| BEC65_RS21815 (BEC65_25090) | 51715..53358 | + | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| BEC65_RS21820 (BEC65_25095) | 53409..54719 | + | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| BEC65_RS21825 (BEC65_25100) | 54703..55197 | + | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| BEC65_RS21830 (BEC65_25105) | 55222..56760 | + | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| BEC65_RS21835 (BEC65_25110) | 56751..57860 | + | 1110 | WP_000974903 | type II secretion system F family protein | - |
| BEC65_RS21840 (BEC65_25115) | 57910..58473 | + | 564 | WP_034169414 | type 4 pilus major pilin | - |
| BEC65_RS21845 (BEC65_25120) | 58539..59021 | + | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| BEC65_RS21850 (BEC65_25125) | 59025..59660 | + | 636 | WP_000934977 | A24 family peptidase | - |
| BEC65_RS21855 (BEC65_25130) | 59673..60713 | + | 1041 | WP_000750515 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
Host bacterium
| ID | 1535 | GenBank | NZ_MCCT01000063 |
| Plasmid name | pZJ807 | Incompatibility group | IncI2 |
| Plasmid size | 60713 bp | Coordinate of oriT [Strand] | 15501..15553 [+] |
| Host baterium | Escherichia coli strain ZJ807 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |