Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101089 |
| Name | oriT_pZJ258  |
| Organism | Escherichia coli strain ZJ258 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MCCG01000066 (15372..15424 [+], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pZJ258
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 672 | GenBank | WP_015059539 |
| Name | t4cp2_BEC51_RS19225_pZJ258 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 36200..60344
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BEC51_RS19080 (BEC51_22805) | 32112..32564 | + | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
| BEC51_RS19085 (BEC51_22810) | 32557..32772 | + | 216 | WP_001127357 | DUF1187 family protein | - |
| BEC51_RS19090 | 32765..32941 | + | 177 | WP_000753050 | hypothetical protein | - |
| BEC51_RS19095 (BEC51_22815) | 32968..33921 | + | 954 | WP_086669491 | SPFH domain-containing protein | - |
| BEC51_RS19100 | 34295..34738 | + | 444 | WP_000964330 | NfeD family protein | - |
| BEC51_RS19105 | 34742..34912 | + | 171 | WP_000550720 | hypothetical protein | - |
| BEC51_RS19110 (BEC51_22825) | 34923..35369 | + | 447 | WP_001243165 | hypothetical protein | - |
| BEC51_RS19115 (BEC51_22830) | 35402..35659 | + | 258 | WP_001542015 | hypothetical protein | - |
| BEC51_RS19120 | 35640..35942 | + | 303 | WP_001360345 | hypothetical protein | - |
| BEC51_RS19125 (BEC51_22835) | 35939..36196 | + | 258 | WP_000739144 | hypothetical protein | - |
| BEC51_RS19130 (BEC51_22840) | 36200..37195 | + | 996 | WP_065304446 | type IV secretion system protein | virB6 |
| BEC51_RS19135 (BEC51_22845) | 37201..37845 | + | 645 | WP_001310442 | type IV secretion system protein | - |
| BEC51_RS19140 (BEC51_22850) | 37854..38078 | + | 225 | WP_000713562 | EexN family lipoprotein | - |
| BEC51_RS19145 (BEC51_22855) | 38301..39110 | - | 810 | WP_171004827 | DUF5710 domain-containing protein | - |
| BEC51_RS19150 (BEC51_22860) | 39158..39793 | + | 636 | WP_015059536 | hypothetical protein | - |
| BEC51_RS19155 (BEC51_22865) | 39893..40144 | + | 252 | WP_000121741 | hypothetical protein | - |
| BEC51_RS19160 (BEC51_22870) | 40134..40415 | + | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| BEC51_RS19165 (BEC51_22875) | 40483..40782 | + | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| BEC51_RS19170 (BEC51_22880) | 40785..42020 | + | 1236 | WP_072652802 | TcpQ domain-containing protein | - |
| BEC51_RS19175 (BEC51_22885) | 42026..42463 | + | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| BEC51_RS19180 (BEC51_22890) | 42582..42980 | + | 399 | WP_001153665 | hypothetical protein | - |
| BEC51_RS19185 (BEC51_22895) | 43001..43585 | + | 585 | WP_001177113 | lytic transglycosylase domain-containing protein | virB1 |
| BEC51_RS19190 (BEC51_22900) | 43585..43875 | + | 291 | WP_000865479 | conjugal transfer protein | - |
| BEC51_RS19195 (BEC51_22905) | 43946..44266 | + | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| BEC51_RS19200 (BEC51_22910) | 44272..46629 | + | 2358 | WP_000548955 | VirB4 family type IV secretion system protein | virb4 |
| BEC51_RS19205 (BEC51_22920) | 46795..47529 | + | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| BEC51_RS19210 (BEC51_22925) | 47595..48296 | + | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| BEC51_RS19215 (BEC51_22930) | 48286..49425 | + | 1140 | WP_000790640 | TrbI/VirB10 family protein | virB10 |
| BEC51_RS19220 (BEC51_22935) | 49444..50499 | + | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| BEC51_RS19225 (BEC51_22940) | 50515..52473 | + | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| BEC51_RS19230 (BEC51_22945) | 52520..53056 | + | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| BEC51_RS19235 (BEC51_22950) | 53049..54692 | + | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| BEC51_RS19240 (BEC51_22955) | 54743..56053 | + | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| BEC51_RS19245 (BEC51_22960) | 56037..56531 | + | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| BEC51_RS19250 (BEC51_22965) | 56556..58094 | + | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| BEC51_RS19255 (BEC51_22970) | 58085..59194 | + | 1110 | WP_000974903 | type II secretion system F family protein | - |
| BEC51_RS19260 (BEC51_22975) | 59239..59796 | + | 558 | WP_000095048 | type 4 pilus major pilin | - |
| BEC51_RS19265 (BEC51_22980) | 59862..60344 | + | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| BEC51_RS19270 (BEC51_22985) | 60348..60983 | + | 636 | WP_000934977 | A24 family peptidase | - |
| BEC51_RS19275 (BEC51_22990) | 60996..62030 | + | 1035 | WP_000750512 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
Host bacterium
| ID | 1533 | GenBank | NZ_MCCG01000066 |
| Plasmid name | pZJ258 | Incompatibility group | IncI2 |
| Plasmid size | 62030 bp | Coordinate of oriT [Strand] | 15372..15424 [+] |
| Host baterium | Escherichia coli strain ZJ258 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |