Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101088 |
| Name | oriT_pZJ48  |
| Organism | Escherichia coli strain ZJ48 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MCCH01000040 (43467..43519 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pZJ48
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 671 | GenBank | WP_015059539 |
| Name | t4cp2_BEC52_RS17170_pZJ48 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1687..24517
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BEC52_RS17120 (BEC52_26230) | 1..1035 | - | 1035 | WP_000750512 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| BEC52_RS17125 (BEC52_26235) | 1048..1683 | - | 636 | WP_000934977 | A24 family peptidase | - |
| BEC52_RS17130 (BEC52_26240) | 1687..2169 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| BEC52_RS17135 (BEC52_26245) | 2235..2798 | - | 564 | WP_034169414 | type 4 pilus major pilin | - |
| BEC52_RS17140 (BEC52_26250) | 2848..3957 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| BEC52_RS17145 (BEC52_26255) | 3948..5486 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| BEC52_RS17150 (BEC52_26260) | 5511..6005 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| BEC52_RS17155 (BEC52_26265) | 5989..7299 | - | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| BEC52_RS17160 (BEC52_26270) | 7350..8993 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| BEC52_RS17165 (BEC52_26275) | 8986..9522 | - | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| BEC52_RS17170 (BEC52_26280) | 9569..11527 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| BEC52_RS17175 (BEC52_26285) | 11543..12598 | - | 1056 | WP_001542006 | P-type DNA transfer ATPase VirB11 | virB11 |
| BEC52_RS17180 (BEC52_26290) | 12617..13756 | - | 1140 | WP_034169415 | TrbI/VirB10 family protein | virB10 |
| BEC52_RS17185 (BEC52_26295) | 13746..14447 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| BEC52_RS17190 (BEC52_26300) | 14513..15247 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| BEC52_RS17195 (BEC52_26310) | 15413..17770 | - | 2358 | WP_000548950 | VirB4 family type IV secretion system protein | virb4 |
| BEC52_RS17200 (BEC52_26315) | 17776..18096 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| BEC52_RS17205 (BEC52_26320) | 18167..18457 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| BEC52_RS17210 (BEC52_26325) | 18457..19041 | - | 585 | WP_001177117 | lytic transglycosylase domain-containing protein | virB1 |
| BEC52_RS17215 (BEC52_26330) | 19062..19460 | - | 399 | WP_001153669 | hypothetical protein | - |
| BEC52_RS17220 (BEC52_26335) | 19579..20016 | - | 438 | WP_034169416 | type IV pilus biogenesis protein PilM | - |
| BEC52_RS17225 (BEC52_26340) | 20022..21257 | - | 1236 | WP_034169417 | toxin co-regulated pilus biosynthesis Q family protein | - |
| BEC52_RS17230 (BEC52_26345) | 21260..21559 | - | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| BEC52_RS17235 (BEC52_26350) | 21607..22416 | + | 810 | WP_024237698 | DUF5710 domain-containing protein | - |
| BEC52_RS17240 (BEC52_26355) | 22639..22863 | - | 225 | WP_000713562 | EexN family lipoprotein | - |
| BEC52_RS17245 (BEC52_26360) | 22872..23516 | - | 645 | WP_001310442 | type IV secretion system protein | - |
| BEC52_RS17250 (BEC52_26365) | 23522..24517 | - | 996 | WP_001028540 | type IV secretion system protein | virB6 |
| BEC52_RS17255 (BEC52_26370) | 24521..24778 | - | 258 | WP_000739144 | hypothetical protein | - |
| BEC52_RS17260 | 24775..25077 | - | 303 | WP_001360345 | hypothetical protein | - |
| BEC52_RS17265 (BEC52_26375) | 25058..25315 | - | 258 | WP_001542015 | hypothetical protein | - |
| BEC52_RS17270 (BEC52_26380) | 25348..25794 | - | 447 | WP_001243165 | hypothetical protein | - |
| BEC52_RS17275 | 25805..25975 | - | 171 | WP_000550720 | hypothetical protein | - |
| BEC52_RS17280 | 25979..26422 | - | 444 | WP_000964330 | NfeD family protein | - |
| BEC52_RS17285 (BEC52_26390) | 26796..27749 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| BEC52_RS17290 | 27776..27952 | - | 177 | WP_000753050 | hypothetical protein | - |
| BEC52_RS17295 (BEC52_26395) | 27945..28160 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| BEC52_RS17300 (BEC52_26400) | 28153..28605 | - | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 1532 | GenBank | NZ_MCCH01000040 |
| Plasmid name | pZJ48 | Incompatibility group | IncI2 |
| Plasmid size | 59072 bp | Coordinate of oriT [Strand] | 43467..43519 [-] |
| Host baterium | Escherichia coli strain ZJ48 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |