Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101086 |
| Name | oriT_pZJ49  |
| Organism | Escherichia coli strain ZJ49 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MCCY01000048 (16405..16457 [+], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pZJ49
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 670 | GenBank | WP_015059539 |
| Name | t4cp2_BEC70_RS23685_pZJ49 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 35407..58237
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BEC70_RS23555 (BEC70_26705) | 31319..31771 | + | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
| BEC70_RS23560 (BEC70_26710) | 31764..31979 | + | 216 | WP_001127357 | DUF1187 family protein | - |
| BEC70_RS23565 | 31972..32148 | + | 177 | WP_000753050 | hypothetical protein | - |
| BEC70_RS23570 (BEC70_26715) | 32175..33128 | + | 954 | WP_072089442 | SPFH domain-containing protein | - |
| BEC70_RS23575 | 33502..33945 | + | 444 | WP_000964330 | NfeD family protein | - |
| BEC70_RS23580 | 33949..34119 | + | 171 | WP_000550720 | hypothetical protein | - |
| BEC70_RS23585 (BEC70_26725) | 34130..34576 | + | 447 | WP_001243165 | hypothetical protein | - |
| BEC70_RS23590 (BEC70_26730) | 34609..34866 | + | 258 | WP_001542015 | hypothetical protein | - |
| BEC70_RS23595 | 34847..35149 | + | 303 | WP_001360345 | hypothetical protein | - |
| BEC70_RS23600 (BEC70_26735) | 35146..35403 | + | 258 | WP_000739144 | hypothetical protein | - |
| BEC70_RS23605 (BEC70_26740) | 35407..36402 | + | 996 | WP_001028540 | type IV secretion system protein | virB6 |
| BEC70_RS23610 (BEC70_26745) | 36408..37052 | + | 645 | WP_001310442 | type IV secretion system protein | - |
| BEC70_RS23615 (BEC70_26750) | 37061..37285 | + | 225 | WP_000713562 | EexN family lipoprotein | - |
| BEC70_RS23620 (BEC70_26755) | 37508..38317 | - | 810 | WP_024237698 | DUF5710 domain-containing protein | - |
| BEC70_RS23625 (BEC70_26760) | 38365..38664 | + | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| BEC70_RS23630 (BEC70_26765) | 38667..39902 | + | 1236 | WP_034169417 | toxin co-regulated pilus biosynthesis Q family protein | - |
| BEC70_RS23635 (BEC70_26770) | 39908..40345 | + | 438 | WP_034169416 | type IV pilus biogenesis protein PilM | - |
| BEC70_RS23640 (BEC70_26775) | 40464..40862 | + | 399 | WP_001153669 | hypothetical protein | - |
| BEC70_RS23645 (BEC70_26780) | 40883..41467 | + | 585 | WP_001177117 | lytic transglycosylase domain-containing protein | virB1 |
| BEC70_RS23650 (BEC70_26785) | 41467..41757 | + | 291 | WP_000865479 | conjugal transfer protein | - |
| BEC70_RS23655 (BEC70_26790) | 41828..42148 | + | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| BEC70_RS23660 (BEC70_26795) | 42154..44511 | + | 2358 | WP_000548950 | VirB4 family type IV secretion system protein | virb4 |
| BEC70_RS23665 (BEC70_26805) | 44677..45411 | + | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| BEC70_RS23670 (BEC70_26810) | 45477..46178 | + | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| BEC70_RS23675 (BEC70_26815) | 46168..47307 | + | 1140 | WP_034169415 | TrbI/VirB10 family protein | virB10 |
| BEC70_RS23680 (BEC70_26820) | 47326..48381 | + | 1056 | WP_001542006 | P-type DNA transfer ATPase VirB11 | virB11 |
| BEC70_RS23685 (BEC70_26825) | 48397..50355 | + | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| BEC70_RS23690 (BEC70_26830) | 50402..50938 | + | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| BEC70_RS23695 (BEC70_26835) | 50931..52574 | + | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| BEC70_RS23700 (BEC70_26840) | 52625..53935 | + | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| BEC70_RS23705 (BEC70_26845) | 53919..54413 | + | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| BEC70_RS23710 (BEC70_26850) | 54438..55976 | + | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| BEC70_RS23715 (BEC70_26855) | 55967..57076 | + | 1110 | WP_000974903 | type II secretion system F family protein | - |
| BEC70_RS23720 (BEC70_26860) | 57126..57689 | + | 564 | WP_034169414 | type 4 pilus major pilin | - |
| BEC70_RS23725 (BEC70_26865) | 57755..58237 | + | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| BEC70_RS23730 (BEC70_26870) | 58241..58876 | + | 636 | WP_000934977 | A24 family peptidase | - |
| BEC70_RS23735 (BEC70_26875) | 58889..59923 | + | 1035 | WP_000750512 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
Host bacterium
| ID | 1530 | GenBank | NZ_MCCY01000048 |
| Plasmid name | pZJ49 | Incompatibility group | IncI2 |
| Plasmid size | 59923 bp | Coordinate of oriT [Strand] | 16405..16457 [+] |
| Host baterium | Escherichia coli strain ZJ49 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |