Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101079 |
| Name | oriT_prep22  |
| Organism | Enterococcus faecium strain BR-MHR218 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JAODSZ010000032 (16890..17092 [-], 203 nt) |
| oriT length | 203 nt |
| IRs (inverted repeats) | 125..130, 143..148 (AAATCC..GGATTT) 66..72, 83..89 (ACCCCCC..GGGGGGT) 33..38, 48..53 (TGGCTG..CAGCCA) |
| Location of nic site | 135..136 |
| Conserved sequence flanking the nic site |
TTTGGTTACA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 203 nt
>oriT_prep22
AAGCGGAAGTCGCAGGTGTGGACTAATCTTGCTGGCTGGTGTGGCGACAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGGCAAAAAAATAGTAGAAAATCCTTTGGTTACAAGGGATTTAGAAAATTTCGGTGTATGTCAAATGAGCTTTAAAAGTTGACATACGCCTTTTTGA
AAGCGGAAGTCGCAGGTGTGGACTAATCTTGCTGGCTGGTGTGGCGACAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGGCAAAAAAATAGTAGAAAATCCTTTGGTTACAAGGGATTTAGAAAATTTCGGTGTATGTCAAATGAGCTTTAAAAGTTGACATACGCCTTTTTGA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1118 | GenBank | WP_025186485 |
| Name | mobT_N6B80_RS10720_prep22 |
UniProt ID | A0A5N0YRV8 |
| Length | 401 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 401 a.a. Molecular weight: 47190.89 Da Isoelectric Point: 6.9549
>WP_025186485.1 MULTISPECIES: MobT family relaxase [Bacillota]
MGGISLNEQTWVQHLKEKRLSYGLSQNRLAIATGITRQYLSDIETGKVKPSDELQLALLETLERFNPDAP
LEMLFDYVRIRFPTTDVQHVVEDVLRLKLSYMLHEDYGFYSYTEHYALGNIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDALVASGVMKRLDLAINDKTGILNIPILTEKCRQEECISVFRSFKSYRS
GELVRKDEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
AYDNPEHTAFKIINRYIRFVDKDDSKARSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAITLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITNKK
MGGISLNEQTWVQHLKEKRLSYGLSQNRLAIATGITRQYLSDIETGKVKPSDELQLALLETLERFNPDAP
LEMLFDYVRIRFPTTDVQHVVEDVLRLKLSYMLHEDYGFYSYTEHYALGNIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDALVASGVMKRLDLAINDKTGILNIPILTEKCRQEECISVFRSFKSYRS
GELVRKDEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
AYDNPEHTAFKIINRYIRFVDKDDSKARSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAITLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITNKK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5N0YRV8 |
T4CP
| ID | 667 | GenBank | WP_002324557 |
| Name | tcpA_N6B80_RS10730_prep22 |
UniProt ID | _ |
| Length | 461 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 461 a.a. Molecular weight: 53440.27 Da Isoelectric Point: 8.2601
>WP_002324557.1 MULTISPECIES: FtsK/SpoIIIE domain-containing protein [Bacillota]
MKQRGKRIRPSDKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDLDLLQVDKIDIPYLVISFSVAIL
VCLLGAFIFRRYKYDMFKQLQHRQKLAKMILENKWYESEQVKTDGFFKDSTSRTKEKITYFPKMYYRLKD
GLISIRVEIMLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIANRLSIDEVQAKDGKLRL
MTNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTNSKLTILDPKNADLADLGSVMGNVYYRKDDML
SCIDRFYDEMMKRSEVMKKMENYKTGENYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVLNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKQIKGRGYVDVGTSV
ISEFYTPLVPKGHDFLEEIKRLSHSKQDTQATCEAEVAGVD
MKQRGKRIRPSDKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDLDLLQVDKIDIPYLVISFSVAIL
VCLLGAFIFRRYKYDMFKQLQHRQKLAKMILENKWYESEQVKTDGFFKDSTSRTKEKITYFPKMYYRLKD
GLISIRVEIMLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIANRLSIDEVQAKDGKLRL
MTNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTNSKLTILDPKNADLADLGSVMGNVYYRKDDML
SCIDRFYDEMMKRSEVMKKMENYKTGENYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVLNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKQIKGRGYVDVGTSV
ISEFYTPLVPKGHDFLEEIKRLSHSKQDTQATCEAEVAGVD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 11705..19208
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| N6B80_RS10670 (N6B80_10670) | 6787..7428 | - | 642 | Protein_9 | plasmid recombination protein | - |
| N6B80_RS10675 (N6B80_10675) | 7834..9210 | - | 1377 | WP_002343717 | tetracycline efflux MFS transporter Tet(L) | - |
| N6B80_RS10680 (N6B80_10680) | 9409..11328 | - | 1920 | WP_002343718 | tetracycline resistance ribosomal protection protein Tet(M) | - |
| N6B80_RS10685 (N6B80_10685) | 11344..11460 | - | 117 | WP_001791010 | tetracycline resistance determinant leader peptide | - |
| N6B80_RS10690 (N6B80_10690) | 11705..12637 | - | 933 | WP_001224318 | conjugal transfer protein | orf13 |
| N6B80_RS10695 (N6B80_10695) | 12634..13635 | - | 1002 | WP_000769868 | bifunctional lysozyme/C40 family peptidase | orf14 |
| N6B80_RS10700 (N6B80_10700) | 13632..14444 | - | 813 | Protein_15 | hypothetical protein | - |
| N6B80_RS10705 (N6B80_10705) | 14434..14718 | - | 285 | Protein_16 | conjugal transfer protein | - |
| N6B80_RS10710 (N6B80_10710) | 14807..15304 | - | 498 | WP_241916636 | antirestriction protein ArdA | - |
| N6B80_RS10715 (N6B80_10715) | 15421..15642 | - | 222 | WP_001009056 | hypothetical protein | orf19 |
| N6B80_RS10720 (N6B80_10720) | 15685..16890 | - | 1206 | WP_025186485 | MobT family relaxase | - |
| N6B80_RS10725 (N6B80_10725) | 16912..17064 | - | 153 | WP_011860839 | hypothetical protein | - |
| N6B80_RS10730 (N6B80_10730) | 17067..18452 | - | 1386 | WP_002324557 | FtsK/SpoIIIE domain-containing protein | virb4 |
| N6B80_RS10735 (N6B80_10735) | 18486..18872 | - | 387 | WP_002324558 | YdcP family protein | orf23 |
| N6B80_RS10740 (N6B80_10740) | 18894..19208 | - | 315 | WP_002324559 | YdcP family protein | orf23 |
| N6B80_RS10745 (N6B80_10745) | 19511..19864 | - | 354 | Protein_24 | Fic family protein | - |
Host bacterium
| ID | 1523 | GenBank | NZ_JAODSZ010000032 |
| Plasmid name | prep22 | Incompatibility group | - |
| Plasmid size | 20339 bp | Coordinate of oriT [Strand] | 16890..17092 [-] |
| Host baterium | Enterococcus faecium strain BR-MHR218 |
Cargo genes
| Drug resistance gene | tet(L), tet(M) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |