Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101058 |
| Name | oriT_pIncI2  |
| Organism | Escherichia coli strain PH-2670-18 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_WLVN01000011 (13731..13783 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pIncI2
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 653 | GenBank | WP_015059539 |
| Name | t4cp2_GKM64_RS24155_pIncI2 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 32911..55741
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GKM64_RS24095 (GKM64_24070) | 28133..29257 | - | 1125 | WP_000486716 | site-specific integrase | - |
| GKM64_RS24100 (GKM64_24075) | 29662..29817 | + | 156 | WP_001358489 | hypothetical protein | - |
| GKM64_RS25265 | 30665..30886 | + | 222 | Protein_37 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| GKM64_RS24105 (GKM64_24080) | 30883..32259 | - | 1377 | WP_000750519 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| GKM64_RS24110 (GKM64_24085) | 32272..32907 | - | 636 | WP_000934977 | A24 family peptidase | - |
| GKM64_RS24115 (GKM64_24090) | 32911..33393 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| GKM64_RS24120 (GKM64_24095) | 33459..34022 | - | 564 | WP_034169414 | type 4 pilus major pilin | - |
| GKM64_RS24125 (GKM64_24100) | 34072..35181 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| GKM64_RS24130 (GKM64_24105) | 35172..36710 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| GKM64_RS24135 (GKM64_24110) | 36735..37229 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| GKM64_RS24140 (GKM64_24115) | 37213..38523 | - | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| GKM64_RS24145 (GKM64_24120) | 38574..40217 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| GKM64_RS24150 (GKM64_24125) | 40210..40746 | - | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| GKM64_RS24155 (GKM64_24130) | 40793..42751 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| GKM64_RS24160 (GKM64_24135) | 42767..43822 | - | 1056 | WP_001542006 | P-type DNA transfer ATPase VirB11 | virB11 |
| GKM64_RS24165 (GKM64_24140) | 43841..44980 | - | 1140 | WP_034169415 | TrbI/VirB10 family protein | virB10 |
| GKM64_RS24170 (GKM64_24145) | 44970..45671 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| GKM64_RS24175 (GKM64_24150) | 45737..46471 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| GKM64_RS24180 (GKM64_24155) | 46637..48994 | - | 2358 | WP_000548950 | VirB4 family type IV secretion system protein | virb4 |
| GKM64_RS24185 (GKM64_24160) | 49000..49320 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| GKM64_RS25090 | 49391..49681 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| GKM64_RS24195 (GKM64_24170) | 49681..50265 | - | 585 | WP_001177117 | lytic transglycosylase domain-containing protein | virB1 |
| GKM64_RS24200 (GKM64_24175) | 50286..50684 | - | 399 | WP_001153669 | hypothetical protein | - |
| GKM64_RS24205 (GKM64_24180) | 50803..51240 | - | 438 | WP_034169416 | type IV pilus biogenesis protein PilM | - |
| GKM64_RS24210 (GKM64_24185) | 51246..52481 | - | 1236 | WP_034169417 | toxin co-regulated pilus biosynthesis Q family protein | - |
| GKM64_RS24215 (GKM64_24190) | 52484..52783 | - | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| GKM64_RS24220 (GKM64_24195) | 52831..53640 | + | 810 | WP_024237698 | DUF5710 domain-containing protein | - |
| GKM64_RS24225 (GKM64_24200) | 53863..54087 | - | 225 | WP_000713562 | EexN family lipoprotein | - |
| GKM64_RS24230 (GKM64_24205) | 54096..54740 | - | 645 | WP_001310442 | type IV secretion system protein | - |
| GKM64_RS24235 (GKM64_24210) | 54746..55741 | - | 996 | WP_001028540 | type IV secretion system protein | virB6 |
| GKM64_RS24240 (GKM64_24215) | 55745..56002 | - | 258 | WP_000739144 | hypothetical protein | - |
| GKM64_RS24245 (GKM64_24220) | 55999..56301 | - | 303 | WP_001360345 | hypothetical protein | - |
| GKM64_RS24250 (GKM64_24225) | 56282..56539 | - | 258 | WP_001542015 | hypothetical protein | - |
| GKM64_RS24255 (GKM64_24230) | 56572..57018 | - | 447 | WP_001243165 | hypothetical protein | - |
| GKM64_RS24655 | 57029..57199 | - | 171 | WP_000550720 | hypothetical protein | - |
| GKM64_RS24260 (GKM64_24235) | 57203..57646 | - | 444 | WP_000964330 | NfeD family protein | - |
| GKM64_RS24265 (GKM64_24240) | 58020..58973 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| GKM64_RS24660 | 59000..59176 | - | 177 | WP_000753050 | hypothetical protein | - |
| GKM64_RS24270 (GKM64_24245) | 59169..59384 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| GKM64_RS24275 (GKM64_24250) | 59377..59829 | - | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 1502 | GenBank | NZ_WLVN01000011 |
| Plasmid name | pIncI2 | Incompatibility group | IncI2 |
| Plasmid size | 60960 bp | Coordinate of oriT [Strand] | 13731..13783 [-] |
| Host baterium | Escherichia coli strain PH-2670-18 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |