Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101032
Name   oriT_pBFTM10 experimental
Organism   Bacteroides fragilis conjugal transfer (of antibiotic-resistance factors) protein (btgA and btgB) genes
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   M77806 (334..638 [+], 305 nt)
oriT length   305 nt
IRs (inverted repeats)    
Location of nic site      147..148
Conserved sequence flanking the
  nic site    		  
Note   

  oriT sequence  


Download         Length: 305 nt

>oriT_pBFTM10
AGGTTCTTTCTTAGTGCCTCGCTCCGACTTCTCCTCGTCCCCCTTGTACGAGCTTTCGCCGTACCGCAGGGGAGGGTGTTCGTCGATTGCTCGGCAGTCGAAAAGAACAGCAAGAGGAAACCGGGCTTTGCCCTGTTTTTCCTCTTGCCCTACGGGATTACGGCTGAATATCAGCCGTTTAAGTTTTTTAACCACAATTAGTTTGAAATTGTGGTTAAAATTATACCTTTGTAAATCAGAGATTTAATAATATAATTAGCAATTAAAATCGCAATTGTATGGATAAAGAAACAACCACTTCTGTC

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1101 GenBank   AAA22902
Name   BtgA_pBFTM10 experimental UniProt ID   Q45137
Length   201 a.a. PDB ID   
Note   

  Relaxase protein sequence


Download         Length: 201 a.a.        Molecular weight: 23232.71 Da        Isoelectric Point: 9.4573

>AAA22902.1 putative [Bacteroides fragilis]
MDKETTTSVTIDRKTFARLDRLAKSNNVSKKDFLSCALEYFEKYGINPVEHESPAKEMQKLIKRCDQVIA
FIRKQEQDFLRPACEAMGSTSMRVTMSMDSILTEKKFSQYQKDNDLFMRDLASLAGIREQALDRAEKVVG
QSRDMLLKNQQAIYARLEAVTQRQEKIFSYIASYIDAKGKAGLFDDIKALYKDEKNKREKI

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB Q45137


Auxiliary protein

ID   398 GenBank   AAA22903
Name   BtgB_pBFTM10 experimental UniProt ID   Q45138
Length   292 a.a. PDB ID   _
Note   

  Auxiliary protein sequence


Download         Length: 292 a.a.        Molecular weight: 33769.97 Da        Isoelectric Point: 9.5204

>AAA22903.1 putative [Bacteroides fragilis]
MNVKIQGGGNGTYANTGSCVAVTNYLQHEDLERMKKGEEVQPFFNQFRDYVSAREVTFKIDNNKAKLSRT
DAKFYVITVSPSEKELRCMGRTPQERAEALQRYIRQDVMRNYAEGFGKGLRSDDVEYYAKIHFNRDGDSD
SDMHAHIIVSRKDRSNTKKLSPKTNHTGKKNCGNVKGGFDRTDFFRKCESSFDRCMGFDREPEESFDYLN
AVKNGSPAEIARQVERAERIRKEKWDNLKAELQSRQEPEKSKIQQVEKTPDIEQPIPKKKQQEEDLELLK
KPKRSRGFGMGM

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5712) (DUF5712)

(4-274)


  Protein structure


Source ID Structure
AlphaFold DB Q45138


Host bacterium

ID   1494 GenBank   M77806
Plasmid name   pBFTM10 Incompatibility group   -
Plasmid size   2162 bp Coordinate of oriT [Strand]   334..638 [+]
Host baterium   Bacteroides fragilis conjugal transfer (of antibiotic-resistance factors) protein (btgA and btgB) genes

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -