Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101031
Name   oriT_RA3 experimental
Organism   Aeromonas hydrophila
Sequence Completeness      minimal
NCBI accession of oriT (coordinates [strand])   DQ401103 (9736..9782 [+], 47 nt)
oriT length   47 nt
IRs (inverted repeats)    
Location of nic site      12..13
Conserved sequence flanking the
  nic site    		  CGTCCTAA
Note   

  oriT sequence  


Download         Length: 47 nt

>oriT_RA3
TTAGAACTAGGGCAGGATTCGCAATTTGCTAGCGTCTGCCGCCGCTT

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1100 GenBank   ABD64842
Name   NIC_RA3 experimental UniProt ID   Q209N6
Length   331 a.a. PDB ID   
Note   

  Relaxase protein sequence


Download         Length: 331 a.a.        Molecular weight: 36933.95 Da        Isoelectric Point: 10.4681

>ABD64842.1 nickase (plasmid) [Aeromonas hydrophila]
MNKGYDTLAGLLEGKVKTKANKTGDLKGRVKRVTDKASEVMVKVSGAAKGAEHVKAHLDYISRNGKLELE
NERGEVIKGKAAVKEVHKEWTQDGGKRRANTRDTANLVLSMPKGTDPKAVRDSARDFAKGQFGDNYQYVF
ALHTDVDHPHVHLTIKTLGHDGRRLHIKKGDPQKWREGFAEQLQRRGIDAEATPRAPRGVVKKAVSQVIK
NIRSRGEIPEVDKAKVKEIVSEFKDKQAGKPPKAKPWEDKIQQRQQEVRKSWLSAAKDLNQGDAEDRELA
SKIVGFVSSMPPLATERHEIAAKLAEQMKPQQKAQDVGQGKAKSERDEDER

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-249)


  Protein structure


Source ID Structure
AlphaFold DB Q209N6


Auxiliary protein

ID   395 GenBank   ABD64841
Name   mobC_RA3 experimental UniProt ID   Q209N7
Length   176 a.a. PDB ID   _
Note   

  Auxiliary protein sequence


Download         Length: 176 a.a.        Molecular weight: 19974.82 Da        Isoelectric Point: 10.2911

>ABD64841.1 putative mobilization protein (plasmid) [Aeromonas hydrophila]
MAKSYRIERRVTKEQKEAWQAFCHAQGVSESDMLGLMIEKVTAGSVALDHKGLKETKTDQLNIRLNTRDM
KRITKRAKAEGFTSRTAWLTAVAMANLNREPVLTESEIQALRTSNRELAAIGRNLNQIAKVLNTEFRESD
KLNRQAVLALSEKIDSHREEVAGLLQRNLNKWGIDE

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(116-138)


  Protein structure


Source ID Structure
AlphaFold DB Q209N7


Host bacterium

ID   1493 GenBank   DQ401103
Plasmid name   pRA3 Incompatibility group   IncU
Plasmid size   45909 bp Coordinate of oriT [Strand]   9736..9782 [+]
Host baterium   Aeromonas hydrophila

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -