Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101029 |
| Name | oriT_pC02-2  |
| Organism | Staphylococcus aureus strain USA300_2014.C02 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP012121 ( 42430..42669 [+], 240 nt) |
| oriT length | 240 nt |
| IRs (inverted repeats) | IR1: 154..160, 164..170 (GTCTGGC..GCCAGAT) IR2: 171..178, 183..190 (CTATCATT..AATGATAG) IR3: 149..153, 194..198 (GGAAT..ATTTC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
CTTATGCTCTT |
| Note |
oriT sequence
Download Length: 240 nt
>oriT_pC02-2
AAGACATTAGTGATAACTGATGTCTTTTTTTATTGTTTTTCTGTAAAAAAGTGCTGTCTTATTTTTGTGACAAATGCTGTATGTAGTGTCACAAAAGTGTGACACTACAGCTTTGTATGATATCACTTTAAAATAACTTAAAACCCTTGGAATGTCTGGCCTTGCCAGATCTATCATTTTTGAATGATAGCAAATTTCCCTTATGCTCTTACGGAGATTTTAGAGTTAAATTAAAATTTC
AAGACATTAGTGATAACTGATGTCTTTTTTTATTGTTTTTCTGTAAAAAAGTGCTGTCTTATTTTTGTGACAAATGCTGTATGTAGTGTCACAAAAGTGTGACACTACAGCTTTGTATGATATCACTTTAAAATAACTTAAAACCCTTGGAATGTCTGGCCTTGCCAGATCTATCATTTTTGAATGATAGCAAATTTCCCTTATGCTCTTACGGAGATTTTAGAGTTAAATTAAAATTTC
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Patrick T LaBreck et al. (2019) Conjugative and replicative biology of the Staphylococcus aureus antimicrobial resistance plasmid, pC02. Plasmid. 102:71-82. [PMID:30844419]
Relaxase
| ID | 1087 | GenBank | WP_031770318 |
| Name | SmpP_pC02  |
UniProt ID | _ |
| Length | 391 a.a. | PDB ID | |
| Note | |||
Relaxase protein sequence
Download Length: 391 a.a. Molecular weight: 47036.61 Da Isoelectric Point: 8.9143
>WP_031770318.1 MULTISPECIES: MobP2 family relaxase [Staphylococcus]
MPKIVTMSEFEQPQNKSNYGNYVNYVDREEAKKNEHEVFQYDVYAHYMFDEQKSNSMFNENSNYMNKNEI
EYTKTKFNEAQKNNGIMWKDVISFDNDSLKEAGIFDPDCKYLDEQKMRQATRKAMQKFEKKEGLENNLVW
SSSIHYNTDNIHVHVAAVEKDVTRERGKRKYSTIKEMKSSFANELFDMSGERTKINNFIRDRIVKGIKEQ
NEPDYNTEMKKQLKKIHEEVKDIPKKEWQYNNNIMKHVRPEIDKFTEMYIKNNHGEEFESFKQDLKKQSK
LYKETYGDKSEYKKYEETKMNDLYSRAGNTILKQLKSFNEEYNGIKYKEPNKLDNKNIPNYNPKATSKMQ
IGYALNNTLYNTQRALRNDFQKERNINQYHYEFDKSYQPEQ
MPKIVTMSEFEQPQNKSNYGNYVNYVDREEAKKNEHEVFQYDVYAHYMFDEQKSNSMFNENSNYMNKNEI
EYTKTKFNEAQKNNGIMWKDVISFDNDSLKEAGIFDPDCKYLDEQKMRQATRKAMQKFEKKEGLENNLVW
SSSIHYNTDNIHVHVAAVEKDVTRERGKRKYSTIKEMKSSFANELFDMSGERTKINNFIRDRIVKGIKEQ
NEPDYNTEMKKQLKKIHEEVKDIPKKEWQYNNNIMKHVRPEIDKFTEMYIKNNHGEEFESFKQDLKKQSK
LYKETYGDKSEYKKYEETKMNDLYSRAGNTILKQLKSFNEEYNGIKYKEPNKLDNKNIPNYNPKATSKMQ
IGYALNNTLYNTQRALRNDFQKERNINQYHYEFDKSYQPEQ
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Reference
[1] Patrick T LaBreck et al. (2019) Conjugative and replicative biology of the Staphylococcus aureus antimicrobial resistance plasmid, pC02. Plasmid. 102:71-82. [PMID:30844419]
Host bacterium
| ID | 1491 | GenBank | NZ_CP012121 |
| Plasmid name | pC02 | Incompatibility group | - |
| Plasmid size | 61539 bp | Coordinate of oriT [Strand] | 10415..10654 [+]; 42430..42669 [+] |
| Host baterium | Staphylococcus aureus strain USA300_2014.C02 |
Cargo genes
| Drug resistance gene | msr(A), mph(C), qacA, blaZ |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |