Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   101028
Name   oriT_pC02-1 experimental
Organism   Staphylococcus aureus strain USA300_2014.C02
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP012121 ( 42430..42669 [+], 240 nt)
oriT length   240 nt
IRs (inverted repeats)      IR1: 154..160, 164..170  (ATCTGGC..GCCAGAT)
  IR2: 171..178, 183..190  (TACCCATT..AATGGGTA)
  IR3: 1..5, 194..198  (GAAAT..ATTTC)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 CTTATGCTCTT
Note   

  oriT sequence  


Download         Length: 240 nt

>oriT_pC02-1
GAAATATACACAATAATAGGGGTGGAACACTTTTGTGACAATTTCAATATATTGTGTCACAAAAGTGTGACAATTTCAATATATTGTGTCACAAAAGTGTGACAATTTAATTTTTGTGACCCCCATAAGATAAATGTTTAAAACCCTTGAAATATCTGGCTTCGCCAGATTACCCATTATAAAATGGGTACATATTTCCCTTATGCTCTTACAAATATCTAGCTATATTAGGAGGTAATA

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Patrick T LaBreck et al. (2019) Conjugative and replicative biology of the Staphylococcus aureus antimicrobial resistance plasmid, pC02. Plasmid. 102:71-82. [PMID:30844419]


Relaxase


ID   1087 GenBank   WP_031770318
Name   SmpP_pC02 insolico UniProt ID   _
Length   391 a.a. PDB ID   
Note   

  Relaxase protein sequence


Download         Length: 391 a.a.        Molecular weight: 47036.61 Da        Isoelectric Point: 8.9143

>WP_031770318.1 MULTISPECIES: MobP2 family relaxase [Staphylococcus]
MPKIVTMSEFEQPQNKSNYGNYVNYVDREEAKKNEHEVFQYDVYAHYMFDEQKSNSMFNENSNYMNKNEI
EYTKTKFNEAQKNNGIMWKDVISFDNDSLKEAGIFDPDCKYLDEQKMRQATRKAMQKFEKKEGLENNLVW
SSSIHYNTDNIHVHVAAVEKDVTRERGKRKYSTIKEMKSSFANELFDMSGERTKINNFIRDRIVKGIKEQ
NEPDYNTEMKKQLKKIHEEVKDIPKKEWQYNNNIMKHVRPEIDKFTEMYIKNNHGEEFESFKQDLKKQSK
LYKETYGDKSEYKKYEETKMNDLYSRAGNTILKQLKSFNEEYNGIKYKEPNKLDNKNIPNYNPKATSKMQ
IGYALNNTLYNTQRALRNDFQKERNINQYHYEFDKSYQPEQ

  Protein domains


Predicted by InterproScan.

(1-363)


  Protein structure



No available structure.



  Reference


[1] Patrick T LaBreck et al. (2019) Conjugative and replicative biology of the Staphylococcus aureus antimicrobial resistance plasmid, pC02. Plasmid. 102:71-82. [PMID:30844419]


Host bacterium


ID   1491 GenBank   NZ_CP012121
Plasmid name   pC02 Incompatibility group   -
Plasmid size   61539 bp Coordinate of oriT [Strand]   10415..10654 [+]; 42430..42669 [+]
Host baterium   Staphylococcus aureus strain USA300_2014.C02

Cargo genes


Drug resistance gene   msr(A), mph(C), qacA, blaZ
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21