Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101025
Name   oriT_pKM101 experimental
Organism   
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   -
oriT length   57 nt
IRs (inverted repeats)      7..12, 19..24  (AGCGTG..CGCGCT)
Location of nic site      35..36
Conserved sequence flanking the
  nic site    		  _
Note   

  oriT sequence  


Download         Length: 57 nt

>oriT_pKM101
TGTGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAA

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Annika Breidenstein et al. (2023) Structural and functional characterization of TraI from pKM101 reveals basis for DNA processing. Life science alliance. 6(4):e202201775. [PMID:36669792]


Relaxase

ID   1081 GenBank   AAB97287
Name   TraI_pKM101 experimental UniProt ID   Q56053
Length   1078 a.a. PDB ID   
Note   

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120119.36 Da        Isoelectric Point: 6.6314

>AAB97287.1 TraI (plasmid) [Salmonella enterica subsp. enterica serovar Typhimurium]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELAKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLSGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
ISGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQRSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
PDB 8A1B
AlphaFold DB Q56053

  Reference


[1] Annika Breidenstein et al. (2023) Structural and functional characterization of TraI from pKM101 reveals basis for DNA processing. Life science alliance. 6(4):e202201775. [PMID:36669792]


Host bacterium

ID   0 GenBank   _
Plasmid name    Incompatibility group   -
Plasmid size    Coordinate of oriT [Strand]   
Host baterium   

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -