Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   101023
Name   oriT_pKPHS3 experimental
Organism   Klebsiella pneumoniae subsp. pneumoniae HS11286
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   CP003225 (103206..103310 [+], 105 nt)
oriT length   105 nt
IRs (inverted repeats)      IR1: 1..6, 8..13  (AATTTG..CAAATT)
  IR2: 46..51, 56..61  (ATTCCA..TGGAAT)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 AGTTATGTGG
Note   

  oriT sequence  


Download         Length: 105 nt

>oriT_pKPHS3
AATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATT

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Jianfeng Zhang et al. (2023) Mobilizable plasmids drive the spread of antimicrobial resistance genes and virulence genes in Klebsiella pneumoniae. Genome medicine. 15(1):106. [PMID:38041146]


Host bacterium


ID   1484 GenBank   CP003225
Plasmid name   pKPHS3 Incompatibility group   IncA/C2
Plasmid size   105974 bp Coordinate of oriT [Strand]   103206..103310 [+]
Host baterium   Klebsiella pneumoniae subsp. pneumoniae HS11286

Cargo genes


Drug resistance gene   resistance to amoxicillin, ampicillin, cephalothin, piperacillin, ticarcillin, amikacin, gentamicin, tobramycin, doxycycline, tetracycline, chloramphenicol, florfenicol, streptomycin, trimethoprim, sulfamethoxazole, aztreonam, cefepime, cefotaxime, ceftazidime and ceftriaxone
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -